通过静态实验,研究了不同反应时间、p H值、初始铀浓度、磷酸二氢钠用量、温度等因素对磷酸二氢钠去除溶液中铀的效果的影响,并结合红外光谱、扫描电镜和热重分析等测试结果,探讨了磷酸二氢钠去除铀的机理.结果表明,一定质量的磷酸二氢钠对铀的去除量随着铀初始浓度的增大而增大,随着温度的升高而减小;在p H值为5,磷酸二氢钠用量为2.0 mg时,磷酸二氢钠去除铀的效果最好,铀的去除率高达99%以上;反应在120 min基本达到平衡.红外光谱分析说明磷酸二氢钠主要是通过磷酸二氢根与UO2+2发生络合反应去除溶液中的铀,扫描电镜分析显示反应生产了矿物晶体,热重分析可知该矿物晶体具有很好的热稳定性.

By the static experiments, the effects of reaction time, temperature, pH, initial concentration of uranium, dosage of sodium dihydrogen phosphate on the removal of uranium by sodium dihydrogen phosphate were investigated. With the analysis results of infrared spectroscopy, scanning electron microscopy and thermogravimetric analysis,the mechanism of the removal reaction was also studied. The results indicated that the removal capacity of sodium dihydrogen phosphate of uranium increased with the increase of initial concentration of uranium, while descended with the ascent of temperature. When pH was 5 and dosage of sodium dihydrogen phosphate was 2.0 mg, the highest removal efficiency of uranium was obtained, and the removal rate reached 99%. Moreover, the reaction equilibrium was achieved at 120 min. In addition, infrared analysis illustrated that sodium dihydrogen phosphate mainly depended on the strong complexation of dihydrogen phosphate roots to remove uranium. Scanning el

目的:研究溶菌酶与乙二胺四乙酸二钠（ethylenediaminetetraacetic acid disodium salt,EDTA二钠）对粪肠球菌和牙髓卟啉单胞菌的协同抑菌作用。方法:分别培养粪肠球菌和牙髓卟啉单胞菌,并调整菌液浓度至108菌落形成单位（colony-forming unit,CFU）/m L;配制终浓度为0.3、0.5、1、2、5、10、50、100、150和300 g/L的单纯溶菌酶抑菌液,以及添加浓度为0.5、1.0、2.0 g/L的EDTA二钠的混合抑菌液。将菌液与抑菌液作用15 min,加入水溶性四唑盐（water-soluble tetrazolium,WST）工作液染色,酶标仪测定光密度值,计算细菌活性。结果:单纯溶菌酶抑菌液（浓度0.5150 g/L）对两种细菌的抑菌作用随溶菌酶浓度的升高而增强,且对粪肠球菌的抑菌作用更显著。EDTA二钠与溶菌酶有协同抑菌作用,与溶菌酶浓度相关。溶菌酶浓度为0.550 g/L时,EDTA二钠协同溶菌酶作用于粪肠球菌（P3.7倍;溶菌酶浓度为0.510 g/L时,EDTA二钠协同溶菌酶作用于牙髓卟啉单胞菌（P3.5倍;当溶菌酶浓度大于100 g/L时,加入EDTA二钠对细菌均没有明显的协同抑菌作用（P>0.05）。结论:对于粪肠球菌及牙髓卟啉单胞菌,在溶菌酶较低浓度时,EDTA二钠有协同抑菌作用;在溶菌酶较高浓度时,EDTA二钠无协同抑菌作用。

Objective:To evaluate the synergistic antibacterial effects of lysozyme with ethylenediami-netetraacetic acid disodium salt (EDTA-2Na) on Enterococcus faecalis (E.faecalis) and Porphyromonas endodontalis ( P.endodontalis) .Methods:E.faecalis and P.endodontalis were cultured and adjusted to 108 CFU/mL.Then 0.3, 0.5, 1, 2, 5, 10, 50, 100, 150 and 300 g/L of lysozyme were prepared with deionized water;and the lysozyme solutions were mixed with 0.5, 1.0, 2.0 g/L of EDTA-2Na, re-spectively.The bacteria and lysosome with/without EDTA-2Na interacted for 15 min, then water-soluble tetrazolium (WST) working solution was added and the activity of the bacteria was calculated by mea-suring optical densities at 450 nm and 630 nm with microplate spectrophotometer .Results:Regarding the pure lysozyme from 0.5 g/L to 150 g/L, more E.faecalis and P.endodontalis were inhibited when the concentration of lysozyme was higher , especially for E.faecalis.There was synergistic effect of lysozyme wit

目的：优化氯磷酸二钠缓释微丸的工艺以及处方中辅料的配方。方法：以20 mg氯膦酸二钠为一个微丸的剂量，优化滚制速度、时间和干燥温度、微晶纤维素和润滑剂的加入量及乙基纤维素和硬脂酸的比例。结果：最佳微丸制备工艺为：高档速度下滚制6 min，干燥温度为60℃。微丸的最佳配方为：20 mg氯膦酸二钠，不加微晶纤维素，乙基纤维素和硬脂酸比例为1∶2，24μl的10％Eudragit L30D-55。结论：该工艺和配方可以作为制备氯磷酸二钠缓释微丸的较佳方案。

Objective: To optimize the preparation and formulation process of clodronate sustained-release pellets. Methods:Taking 20 mg of clodronate as a dose of pellet, the preparation processes such as speed and time of ball rolling, drying temperature and dosage of excipients and lubricant were optimized. Anion exchange chromatography was used to determine the release amount of the clodronate pellets prepared under different conditions at different time points. Results:The best processes for preparation and formulation of pellets were as follows:rolling for 6 min at high speed and 60℃, adding 20 mg of clodronate disodium, ethyl cellulose and stearic acid at ratio of 1:2 and 24 μl of 10%Eudragit L30D-55 without microcrystalline cellulose. Conclusion:The process above-mentioned is a better one for preparation of sustained-release pellets of disodium clodronate.

考察实验室常用的乙二胺四乙酸二钠滴定液(0.05mol/L)在相应贮存条件和日常使用时浓度变化情况,以确定有效期;通过浓度标定考察0~5个月样品浓度相对偏差,得出乙二胺四乙酸二钠滴定液的有效期为3个月。提供了一种准确、可靠、能满足实验要求的滴定液有效期验证方法。

The concentration variation of disodium edetate titrating solution,which is commonly used in laboratories,was evaluated to determine its expiry period under storage and common use conditions.Relative deviation of zero-to-five-month sample concentration was evaluated through concentration titration,and the expiry period of the solution was determined to be three months. This experiment provides an accurate and reliable validation method for expiry period of titrating solution which can satisfy experiment requirements.

目的：观察帕米膦酸二钠对人骨髓间充质干细胞（Mesenchymal stem cells，MSC）生物学特性的影响，以探索双磷酸盐导致骨组织损伤的机制。方法人骨髓MSC培养体系中加入不同浓度的帕米膦酸二钠，培养72 h后，MTT法测定490 nm光密度值，观察细胞增殖情况；培养1周后，流式细胞技术检测细胞表面分子表达。体外诱导MSC成骨分化，体系中加入1μg/mL帕米膦酸二钠，1周后PCR法测定细胞Runx-2表达水平，2周后组织化学法测定细胞内碱性磷酸酶活性，以细胞总蛋白量为参照，观察MSC成骨分化的差异。结果 MTT结果显示，在0.1~10μg/mL浓度范围内，帕米膦酸二钠抑制人骨髓MSC增殖，作用呈浓度依赖性，最低作用浓度为1μg/mL，72 h OD490显著低于对照组（P<0.01）。流式细胞检测显示，帕米膦酸二钠处理后，细胞仍均一表达CD44和CD73，不表达CD31和CD45。PCR及细胞化学结果显示，帕米膦酸二钠无促进MSC成骨分化作用，也未提高成骨诱导体系促分化效果。结论帕米膦酸二钠可抑制MSC增殖，不促进其体外成骨能力，可能是长期使用双磷酸盐导致骨损伤的机制之一。

Objective To observe the effects of Parmidronate disodium on the biological properties of human bone marrow mesenchymal stem cells (MSCs) to shed a light on the mechanisms underlying the damaging activity of alendronate on bone tissue. Methods Parmidronate disodium at graded doses was added into the culture of human bone marrow MSCs and the culture was maintained for 72 hours, MTT test was used to evaluate the cellular proliferation status. Also, the surface marker profile was observed after culture for one week. MSCs were cultured in the presence of parmidronate disodium (0.5μg/mL) for 2 weeks and the cellular activity of alkaline phosphatase was detected. The total cellular protein content was used as the internal reference. Results The results from MTT assay showed that Parmidronate disodium within a range of concentrations (0.1-10 μg/mL) inhibited human MSC growth in a dose-dependent manner;The lowest effective dose was 1.0μg/mL, and the value of OD490 from MSCs cultur