登录

双语推荐：免疫胶体金法

进入期刊文章
目的：建立PCR-免疫胶体金试纸条法快速检测食品中肠出血性大肠杆菌O157：H7的分析方法。方法通过设计特异性引物建立肠出血性大肠杆菌O157：H7 PCR检测方法并使用免疫胶体金技术以及双抗体夹心法建立PCR产物快速检测试纸条并设计核酸检测展开液；将1株大肠杆菌O157： H7标准菌株和7株其他常见食源性致病菌作为试验菌株，用试验菌株检测PCR-免疫胶体金试纸条方法的检测特异度，并比较PCR-免疫胶体金试纸条法和PCR-琼脂糖凝胶电泳法的检测敏感度。结果 PCR-免疫胶体金法具有良好的特异度，灵敏度比标准琼脂糖凝胶电泳法高100倍。结论本文建立的肠出血性大肠杆菌 O157：H7检测 PCR-免疫胶体金试纸条法特异度好，灵敏度高，价格低廉，适用于食品中肠出血性大肠杆菌O157：H7的检测。
ABSTRACT:Objective To establish a PCR-immunogold strip method for rapid detection ofEscherichia coli O157:H7 in food.MethodsThe PCR method was established through the design of specific primers and the establishment of rapid test immunogold strip for detection of PCR products combined immunogold skills with double antibody sandwich method. Test specificity of the new method using the test bacterial strains which include 1Escherichia coli O157:H7 and 7 common food-borne pathogens and compare the sensitivities of the immunogold strip method and the traditional agarose gel electrophoresis method.ResultsThe established method was specific and the sensitivity was higher than the agarose gel electrophoresis by 100 times. ConclusionThe new method is suitable for the detection ofEscherichia coli O157:H7 in food.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
通过两种方法联合检测粪便隐血,为临床消化道少量出血的诊断提供较为可靠的依据。方法:邻甲苯胺法、免疫胶体金法,分别对20例上消化道出血病人的粪便进行检测,对免疫的胶体金法阴性而邻甲苯胺法阳性标本进行分析。结果:化学法敏感性高,但特异性低;免疫法特异性好,但会出现假阴性。结论:在日常工作中化学法对粪便隐血只能起初筛作用,免疫法虽是较为可靠的方法,但对大便明显呈黑色或柏油样,而免疫胶体金法检测为阴性标本,用邻甲苯胺法作为免疫胶体金法的补充试验,进行联合检测,防止漏检,出现假阴性。
Objective:To discuss the detective methods through the combination of two methods in detection of fecal occult blood , it provides a reliable basis for clinical diagnosis of gastrointestinal bleeding .Methods:o-toluidine method , immune colloidal gold method , respectively, were detected in stool of upper gastrointestinal hemorrhage in 20 cases, analysis of samples immune colloidal gold test nega-tive and positive o-toluidine method .Results:Using chemical method has high sensitivity ,but low specificity;method of specific immuni-ty was good, but there would be false negative .Conclusion:In fecal occult blood in the immunochemical method first screen role in daily work, should be a reliable method , but it could black or tarry stool , and negative sample immune colloidal gold method , as a supplement of immune colloidal gold detection using o -toluidine method , joint detection , prevent omissions , false negative .

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：分析化学发光法（微粒子酶免法）与酶联免疫吸附实验（ELISA）及胶体金免疫沉析法检测乙肝表面抗原的差异性。方法：利用雅培i2000化学发光分析仪测定病人标本，记录乙肝表面抗原的定量结果，将同样的标本用酶联免疫吸附实验及胶体金检测，记录检测结果，经统计学处理，对三种方法的测定结果进行比较。结果：三种方法测定乙肝表面抗原的结果存在差异，化学发光法的阳性检出率较ELISA法及胶体金免疫沉析法高。结论：对ELISA法及胶体金免疫沉析法检测乙肝表面抗原处于临界状态的标本，应使用化学发光法检测，以提高结果的准确性，更好的为临床提供诊断依据。
Analysis of chemiluminescence (microparticle enzyme immunoassay method) and enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation, analysis of coloidal gold was detected difference between hepatitis B surface antigen.Methods:Abbot i2000 using chemiluminescence analyzer patient specimens. Record the results of the quantitative hepatitis B surface antigen, Wil have the same specimens by enzyme-linked immunosorbent assay and coloidal gold detection,Record test results, Statistical analysis,Determination of the three methods were compared.Results:Three Methods for measuring the results of hepatitis B surface antigen differences,Chemiluminescence than the positive rate of ELISA ,and immunogold method with high precipitation.Conclusion:On ELISA, and immune precipitation of coloidal gold assay of hepatitis B surface antigen in the critical state of the specimens,Chemiluminescence detection should be used to improve the accuracy of the results,the beter the basis for the clinical d

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：建立食品中单核增生李斯特氏菌快速检测PCR-免疫胶体金试纸条法。方法通过设计特异性引物建立单增李斯特氏菌检测PCR方法并使用免疫胶体金技术建立PCR产物快速检测试纸条；用试验菌株检测PCR-免疫胶体金试纸条方法的检测特异度与敏感度。使用新建方法对市售肉制品和乳制品中单核增生李斯特氏菌进行检测，验证该方法在食品检测中的可行性。结果 PCR-免疫胶体金法具有良好的特异度，敏感度比标准琼脂糖凝胶电泳法高100倍。采集乳品样品131份，阳性样品1份，检出率1.53%；肉制品224份，阳性样品4份，检测率1.79%。结论建立的单增李斯特氏菌检测PCR-免疫胶体金试纸条法特异度好，敏感度高，适用于食品中单增李斯特氏菌的检测。
ABSTRACT:Objective To establish the PCR-immunogold method for detection ofListeria monocytogenes in food.MethodsThe PCR method forListeria monocytogenes detection was built through the design of specific primers and the rapid test immunogold strip for detection of PCR products was established. Then the specificity and sensitivity of the new method were tested using the test bacterial strains andListeria monocytogenes in milk and meat products was detected using the PCR-immunogold strip method to verify whether this method is appropriate to food samples test.ResultsThe method established is specific and the sensitivity is higher than that of the agarose gel electrophoresis by 100 times. We screened 131 milk samples and 224 meat samples totally and found 1 (1.53%) of milk samples and 4 (1.79%) of meat samples carrying Listeria monocytogenes.ConclusionThe new method has high specificity and sensitivity and is suitable for the detection of Listeria monocytogenes in food.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
探讨胶体金法检测无偿献血者乙型肝炎病毒表面抗原(HBs Ag)漏检的原因及预防措施。方法:将胶体金法检测结果为阴性的样本应用酶联免疫吸附试验(ELISA法)检测,对检测结果为阳性的样本再次应用胶体金法进行检测,并对胶体金法漏检的原因进行分析。结果:46 000例无偿献血者ELISA法共检出226例HBs Ag阳性,阳性率0.49%,226例ELISA法阳性样本再次应用胶体金法检测,58例阳性。结论:方法学限制及人为因素是胶体金法筛查HBs Ag漏检的主要原因,严格试剂使用前筛选、考评,提高医务人员检测技能、质量意识及加强责任心可降低胶体金法漏检。
Objective:To explore the undetected reasons and prevention measures of colloidal gold method in the detection of hepatitis B surface antigens(HBsAg) in volunteer donors.Methods:The sample with negative colloidal gold method test result was applied enzyme linked immunosorbent assay(ELISA method) for detection.The sample with positive test result was applied again colloidal gold method for detection.The undetected reasons of colloidal gold method were analyzed.Results:In 46 000 cases of volunteer donors,ELISA method detected 226 cases of HBsAg positive,and the positive rate was 0.49%.226 cases of ELISA method positive samples were applied again colloidal gold method for detection,and 58 cases were positive.Conclusion:Methodological limitations and man-made factors are the undetected main reasons of screening HBsAg by colloidal gold method. Strict reagent screening and evaluation before use,improving medical personnel testing skills and quality awareness,strengthening responsibi

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的了解呼和浩特市乳制品中三聚氰胺残留情况。方法从超市采集69份奶粉制备的休闲乳制品及奶酪和奶皮等传统乳制品,另外采集4个品牌128份液态奶。采用高效薄层色谱法和免疫胶体金检测卡进行三聚氰胺残留检验。结果 197份样品中除了7份奶粉制备的休闲乳制品和2个工厂制作奶酪免疫胶体金法检验结果可疑外,其他样品检验结果均为阴性。结论 高效薄层色谱法虽不如免疫胶体金法灵敏,但检测成本较低,适合进行日常大规模筛查性检验。
Objective To investigate the situation of melamine residue in dairy products in Hohhot. Methods A total of 69 milk powder of leisure dairy food and Mongolian conventional cheese and “milk-skin” samples, as well 128 liquid milk samples of 4 brands were collected from supermarkets of Hohhot city. Melamine residue was test by a high performance thin layer chromatography (HPTLC) method and an immune colloidal gold test kit (ICGT). Results Except 7 milk powder made leisure foods and 2 dairy factory made cheese samples were suspicious (±), the remaining of 197 dairy samples were melamine free (?).Conclusion The HPTLC methods was not as sensitive as the ICGT, however, it is a cheaper method for daily melamine residues screening.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的探讨并评价不同的检验方法对沙眼衣原体检测结果的影响。方法采用酶联免疫吸附测定（ELISA）法与免疫胶体金技术对354例标本进行沙眼衣原体检测,并对两种方法的检测结果进行比较。结果 ELISA与免疫胶体金技术检测结果比较,总体标本及女性标本检测结果的差异无统计学意义（P〉0.05）,ELISA检测男性标本的阳性检出率为11.02%（13/118）,显著高于女性标本[4.2%（5/118）]（P〈0.05）。结论 ELISA法特异性、敏感性高于免疫胶体金技术,对男性尿道沙眼衣原体感染的早期诊断具有重要意义。
Objective To investigate and evaluate the effects of different test methods on the results of Chlamydia trachomatis detection .Methods Enzyme-linked immunosorbent assay(ELISA) method and the immune colloidal gold technique were adopted to detect the Chlamydia trachomatis in 354 specimens .Results Compared the detection results of ELISA and immune colloidal gold technique ,differences of detection rates of overall specimens and female specimens was not statistically significant (P>0 .05) .The positive rate of male specimens detected by ELISA was 11 .02% (13/118) ,which was significantly higher than that of female speci-mens[4 .2% (5/118)](P<0 .05) .Conclusion The specificity and sensitivity of ELISA were higher than those of immune colloidal gold technique ,which is important for the early diagnosis of male urethral Chlamydia trachomatis infection .

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的对胶体金试纸法与化学发光免疫法测定人绒毛膜促性腺激素(HCG)的结果进行研究分析。方法选取2013年1月~2014年1月本院收治的孕妇140例作为研究对象,将孕妇分为胶体金试纸法组与化学发光免疫法组,每组70例孕妇,采用不同的方法测定人绒毛膜促性腺激素,对研究结果进行对比分析。结果化学发光免疫学组阳性率(94.29%)明显好于胶体金试纸法组(68.57%),两组比较差异具有统计学意义(P0.05)。结论测定人绒毛膜促性腺激素(HCG)采用胶体金试纸法测试方便,易操作,但是化学发光免疫学检测法更能准确检测,且灵敏度高,值得在检测中推广应用。
Objective To study and analyze the HCG test results by colloidal gold test strip and chemiluminescence immunoassay.Methods 140 cases of pregnant women from January 2013 to January 2014 in our hospital were chosen as study objects, and they were divided into the group of colloidal gold test strip and the group of chemiluminescence immunoassay, each group with 70 cases. The HCG were tested in different ways, and the results were compared and analyzed.Results The positive rate of the chemical group(94.29%) was better than the test paper group(68.57%), differences between two groups were statistically significant(P<0.05). Conclusion It’s more convenient and easier to test HCG by colloidal gold test strip, but chemiluminescence immunoassay is more accurate with high sensitivity, which is worthy of popularization and application in detection.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的针对茶叶中黄曲霉毒素B1的检测,对比胶体金免疫层析法、高效液相色谱、酶联免疫法的差异。方法以红茶、绿茶、花茶为基质,进行方法验证。并选取具有代表性的红茶、绿茶、乌龙茶、及花茶、萃取茶、药用保健茶分别用不同的方法检测。结果改良后的液相法和胶体金免疫层析法准确度和精密度较高,酶联免疫法存在假阳性。随机检测11种茶叶仅有一种检出黄曲霉毒素B1。
Objectives To compare the three different methods for the determination of aflatoxin B1 in tea samples, including gold immune chromatography assay (GICA), high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). Methods Method validation was done based on black tea, green tea and jasmine tea. And black tea, green tea, oolong tea, jasmine tea, extracted tea, medicinal health tea as representatives were detected with different methods. Results Both of optimized GICA and HPLC achieved high accuracy and precision. ELISA assay produced fake positive. Only one of eleven tested samples had aflatoxin B1 in random test.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的将日本进口的胱抑素C（Cys C）抗体与某公司制备的兔抗Cys C抗体、羊抗Cys C抗体进行质量检测，初步应用试验评估这3种抗体能否用于定量检测血清Cys C （胶体金免疫比浊法）试剂盒的研发。方法分别用紫外分光光度法、二喹啉甲酸（BCA ）法检测3种Cys C抗体浓度；以聚丙烯酰胺凝胶电泳检测其纯度；采用酶联免疫吸附法（ELISA）测效价；然后将这3种Cys C抗体分别标记胶体金颗粒，以胶体金免疫比浊法测定血清Cys C ，比较其初步应用性。结果日本进口Cys C抗体浓度高达15．0～16．0 mg/mL ，某公司自制兔抗Cys C抗体、羊抗Cys C抗体浓度范围在3．0～4．0 mg/mL之间；聚丙烯酰胺凝胶电泳显示3种抗体纯度相近，结果为85％。其抗体效价大于或等于1∶104；胶体金免疫比浊法测定Cys C的临床比对结果证实公司自制抗体与日本进口Cys C抗体有较好的可比性。结论某公司的兔抗Cys C、羊抗Cys C抗体与日本进口Cys C抗体虽浓度不同，但均具有高纯度和高效价的特点，可用于定量检测血清Cys C（胶体金免疫比浊法）试剂盒的研发。
Objective To evaluate quality of three different cystatin C (Cys C) antibodies .Methods Imported Cys Cantibody,rabbit anti-Cys Cantibodyand goat anti-Cys Cantibodymade by a company were detected for con-centration by using ultraviolet spectrophotometry and bicinchonininc acid (BCA) method ,for purity by using polyac-rylamide gel electrophoresis and for titer by using indirect enzyme linked immunosorbent assay .Then the three kinds of Cys Cantibodywere labeled by colloidal gold particles ,and the application value was assessed by detecting serum Cys C concentration in clinical samples .Results The concentration of imported Cys Cantibodywas 15 .0-16 .0 mg/mL ,and that of the company made antibodies were 3 .0 -4 .0 mg/mL .The purities of the three kinds of antibody were similar and reached 85% ,the antibody titer were all at least1 :104 ,and the detected results of Cys C concentra-tion in clinical samples were with fine comparability among the three different test reagents ,made by differ

[ 可能符合您检索需要的词汇 ]