目的对宫腔内夫精人工授精夫妇的丈夫精子资料进行回顾性分析,观察不同精子情况与临床妊娠率的关系。方法选择2004年~2012年因不孕症在中山市博爱医院生殖医学中心进行宫腔内人工授精(IUI)治疗的夫妇1646对共3178个周期。根据丈夫精子分为畸形精子症、畸弱精子症、弱精子症、少精子症、少弱精子症、少弱畸精子症、精浆异常、正常精液8组。观察总结临床妊娠率与丈夫精子情况的关系。结果总体IUI临床妊娠率为12.4%(394/3178)。8组丈夫精子情况与临床妊娠率均无明显关系。结论 IUI临床妊娠率受多种因素影响,与丈夫精子情况无明显相关性。

Objective To determine whether there is any relationship between IUI pregnancy rate and husband’s sperm quality. Methods We carried out this retrospective study on 1646 couples undergoing 3178 intrauterine insemination cycles at Reproductive Medicine Center of Zhong shan Boai Hospital during 2004 to 2012. The relationship between IUI pregnancy rate and husband’s sperm quality was evaluated. Results The overall clinical pregnancy rate was 12.4%(394/3178). We didn’t ifnd the relationship between IUI pregnancy rate and husband’s sperm quality. Conclusion Sperm parameters before sperm preparation couldn’t have predictive value for pregnancy outcome after intrauterine insemination.

目的探讨男性不育患者精子DNA完整性和染色体畸变的发生率及临床意义。方法精液标本来自正常已育男性及不育患者,以WHO标准行精液分析,根据结果及不育史分为对照组、少精症组、弱精症组、少弱精症组。精子染色质扩散实验分析精子DNA损伤率和荧光原位杂交实验分析精子染色体(18、X、Y)非整倍体率。结果少精症组、弱精症组和少弱精症组精子DNA损伤率对照组相比差异有统计学意义(P0.01);不育组的精子染色体(18、X、Y)非整倍体率与对照组比较差异有统计学意义(P0.05);精子DNA损伤率与染色体非整倍体率呈明显正相关(r=0.453,P=0.008)。结论不育患者精子DNA损伤率和染色体非整倍体率较对照组明显升高且呈显著正相关。在减数分裂过程中,精子DNA损伤可能是导致非整倍体精子形成的重要原因之一。

Objective To investigate the changes in sperm DNA integrity and chromosomal abnormalities in some subfertile patients and its clinical significance. Methods Semen samples were obtained from healthy normal and subfertile men. All samples were classified as normal group and subfertile groups( Oligozoospermia, Asthenozo-ospermia and Oligoasthenozoospermia) according to World Health Organization guidelines. The level of DNA dam-age frequency was determined by sperm chromatin dispersion (SCD) test and the chromosomal(18,X,Y) aneu-ploidy ratio was analyzed by fluorescence in situ hybridization (FISH). Results Significant DNA damage frequen-cy differences were found among control group and subfertile groups (P<0.001);and aslo significant differences were noted in sperm chromosomal(18,X,Y) aneuploidy ratio between control group and subfertile groups (P <0.05);there were significant positive correlations between sperm DNA damage frequency and sperm chromosomal aneuploidy ratio (r=0.453,P=0.008)

目的 观察复方玄驹胶囊、麒麟丸治疗少弱精症患者的疗效.方法 采用服药前后自身对照及两种药物相互对照的临床研究方法,对100例少弱精症患者随机分为两组.一组口服复方玄驹胶囊(3粒,3次/日)、一组口服麒麟丸(6g,3次/日),12周为一个疗程.以精子密度和(a+b)级精子为主要疗效指标,以a级精子、精子活动率为次要疗效指标,评价两种药物的效果.结果 有95例患者完成了临床研究,两组在精子密度、(a+b)级精子、a级精子和精子活动率,治疗完成后较治疗前均有显著性差异(P＜0.05).治疗完成后两组在精子密度、(a+b)级精子、a级精子差异有统计学意义(P＜0.05),而在精子活动率上无统计学差异(P＞0.05).结论 两药物对少弱精症患者精液质量的提高均有一定的治疗效果,复方玄驹胶囊对少弱精症的效果较麒麟丸更佳.

Objectives To observe the clinical effects of Fufang Xuanju capsules and Qilin Pills in patients oligoasthenospermia.Methods Using a self-controlled before and after taking one drug and a comparison of two drugs clinical trials,A total of 100 infertile men oligoasthenospermia were equally randomized into two groups.One group was treated with Fufang Xuanju capsules(3,3 times / day),Another was treated with Qilin pills(6g,3 times/ day) for 12 weeks as a course.We evaluated the efficacy of the drugs with sperm concentration and vitality(a + b) as the primary and the number of grade a sperm and sperm motility as the secondary therapeutic indexes.Results A total of 95 patients accomplished the clinical study.Two groups were significantly different before and after treatment in sperm concentration,vitality (a + b),the number of grade a sperm and sperm motility (P ＜ 0.05).The difference was statistically significant in two groups in sperm density,grade(a + b) sperm and the number o

目的：探讨染色体核型异常与男性无精子症、重度少弱精子症的关系。方法对2011年1月至2013年10月来我院生殖中心要求助孕的445例无精子症、147例重度少弱精子症患者，采用外周血常规染色体制备方法对染色体进行核型分析，并采用聚合酶链反应对122例Y染色体进行AZF微缺失检测，283例不育症患者进行睾丸/附睾穿刺取精检查。结果445例无精子症者检出染色体核型异常率为38.65%，分别为性染色体异常22.7%，常染色体异常6.52%、染色体多态性9.43%；147例重度少弱精子症者染色体异常率7.48%；与对照组相比差异均有统计学意义（P＜0.01）。8例Y染色体AZF微缺失位点为AZFa（SY 84，SY 86），AZFb（SY 127，SY 134），AZFc （SY254，SY 255）。结论无精子症、重度少弱精子症与染色体核型异常及Y 染色体微缺失密切相关，有必要在提供辅助生殖技术之前进行遗传学检查。

Objective To detect the frequency and types of chromosomal anomalies for infertile men with azoospermia and severe oligozoospermia. Methods 445 cases of azoospermia and 147 cases of severe oligozoospermia patients in our reproductive center were recruited from January 2011 to October 2013. Karyotype analysis was performed in peripheral blood lymphocytes using standard G-banding. Y chromosome AZF microdeletions were detected using PCR . Testicular sperm retrieval puncture were done in 283 infertility men. Results The frequency of chromosomal abnormalities of azoospermia patients was 38.65%, including sex chromosomes abnormalities (22.7%), autosomals abnormalities (6.52%) and chromosome polymorphism abnormalities (9.43%). The frequency of chromosomal abnormalities of serve oligozoospermia patients was 7.48%. Both abnormal chromosome rates of azoospermia and severe oligoasthenospermia group were significant differences compared with that of the control group (P<0.01). Eight cases of AZF m

目的探讨补肾生精汤治疗少弱精子症的疗效。方法选取本院收治的男性少弱精子症患者120例为研究对象,依据治疗方式分为对照组和观察组,每组60例,对照组口服左卡尼汀口服液,观察组加服补肾生精汤,观察治疗12周后精子质量改善情况。结果治疗前及治疗4周时两组精子总数比较差异无统计学差异(P0.05);治疗12周两组精子总数均明显提高,但观察组精子总数明显高于对照组,差异有统计学意义(t=6.405,P0.01)。治疗前及治疗4周时两组精子活力比较差异无统计学差异(P0.05);治疗12周两组精子活力均明显提高,但观察组精子活力明显高于对照组,差异有统计学意义(t=5.649,P0.01)。结论补肾生精汤联合左卡尼汀治疗少弱精子症患者,可以明显改善精子质量,增加精子总数,提高精子活力。

Objective To investigate the effect of Bushen Shengjing decoction in the treatment of oligozoospermia. Methods Selected 120 male oligoasthenospermia patients in our hospital as the research object. The patients are divided into the control group and the observation group according to their treatment methods. 60 cases in each group. The control group were treated with L-carnitine oral liquid. While the observation group were with Bushen Shengjing decoction. Sperm quality was observed in 12 weeks after treatment. Results There was no significant difference of the sperm counts between the two groups before and after 4 weeks treatment (P > 0.05). Two groups of sperm counts were significantly improved after 12 weeks of treatment, but the sperm counts of the observation group was significant higher than that of the control group, the difference was statistically significant (t=6.405, P 0.05). After 12 weeks treatment, two groups of sperm quality were significantly improved, but the sperm vit