目的：探讨三七花总皂苷清除活性氧自由基及对抗脂质过氧化作用的影响。方法从三七花盘中提取三七花总皂苷，D-101大孔吸附树脂分离纯化皂苷；采用分光光度法测定其对羟基自由基、DPPH 自由基的作用。用硫代巴比妥分光光度法观察三七花总皂苷对 Fe2＋半胱氨酸诱发肝脂质过氧化作用的影响。结果三七花总皂苷对羟自由基、1,1-二苯基-2-三硝基苯肼（DPPH）自由基的半数清除率（IC50值）分别为0.035 mg/ml、0.094 mg/ml；对Fe2＋半胱氨酸诱发肝脂质过氧化的最大抑制率为89.31%，具有较强的抑制作用。结论三七花总皂苷具有清除活性氧自由基及抗脂质过氧化作用。

Objective To investigate the antioxidation activity and resistance of lipid peroxidation of panax notoginseng flower total saponins.Methods The panax notoginseng flower buds were extracted with ethanol. The hydroxyl free radical(?OH), 1,1-diphenyl-2-picrythydrazyl radical(DPPH)clearing rate and resistance of lipid peroxidation of rat liver induced by Fe2+-cysteine were determined by spectrophotometry. Results Half clearance of hydroxyl free radical and DPPH. by panax notoginseng flower total saponin was 0.035 mg/ml and 0.094 mg/ml, the maximum inhibition rate of lipid peroxidation of rat liver induced by Fe2+-cysteine was 89.31%, therefore moderate concentration of extracts had a strong inhibitory effect on lipid peroxidation. Conclusions Panax notoginseng flower total saponins have antioxidant activity and resistance of lipid peroxidation.

考察当归芍药散对实验性高脂血症大鼠脂质过氧化水平及血液流变学指标的影响。方法:以高脂饲料建立高脂血症模型大鼠,观察当归芍药散干预后脂质过氧化水平及血液流变学指标的变化。结果:当归芍药散各给药组能明显减少血清中丙二醛(MDA)的含量,明显增加一氧化氮(NO)含量,升高超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性;红细胞压积、红细胞聚集指数显著降低,红细胞变形指数显著升高。结论:当归芍药散对高脂血症模型大鼠脂质过氧化水平及血液流变性具有一定的改善和治疗作用。

Objective: To observe the effect of Danggui Shaoyao powder on lipid peroxidation level and blood rheology in -dexes of experimental hyperlipidemia rats .Method: Test the lipid peroxidation level and hemorheology index after the in -tervention of Danggui Shaoyao powder using high fat feed hyperlipidemia model rats .Results:Groups taking Danggui Shaoyao powder were obviously reduced the content of MDA , increased NO content and SOD and gsh -px activities. Meantime, red blood cells deposited, erythrocyte aggregation index decreased significantly , and erythrocyte deformation index increased significantly.Conclusion: Danggui Shaoyao powder has an improvement and treatment effect on lipid peroxidation level and blood rheology of hyperlipidemia model rats .

目的：研究蛋清蛋白肽抗氧化作用模式。方法利用超滤技术分离蛋清蛋白木瓜蛋白酶酶解产物；采用 Fenton 体系、邻苯三酚自氧化体系和亚油酸自氧化体系分别测定超滤各组分清除羟自由基、超氧阴离子及抑制脂质过氧化的能力,同时测定各组分对二苯代苦味肼基自由基清除能力(DPPH 自由基)、还原能力及对猪胎儿成纤维细胞(porcine embryonic fibroblast, PEF)过氧化损伤的保护作用。结果超滤各组分中分子量小于3 kDa组分(蛋清蛋白酶解产物-Ⅲ, egg white protein hydrolysate, EWPH-Ⅲ)占蛋清蛋白酶解产物(EWPHs)总量的50.06%。EWPH的抗氧化活性随分子量的降低而增强(P<0.05),其中EWPH-Ⅲ在浓度为5 mg/mL时,对羟自由基、超氧阴离子、DPPH自由基的清除率分别为52.86%、35.05%和78.74%,对亚油酸氧化的抑制率为74.57%。在浓度为2.5 mg/mL时, PEF细胞存活率达到70.06%。结论蛋清蛋白肽具有较强的抗氧化活性且分子量越小,抗氧化活性越强,可以作为氢供体、自由基稳定剂和金属离子螯合剂来抑制过氧化作用。

Objective To investigate antioxidantive action mode of egg white-derived peptides in vitro. Methods The egg white-derived peptides were separated by ultrafiltration from egg white protein hydroly-sates (EWPHs) produced by papain into three fractions. The hydroxyl radical, superoxide anion scavenging ac-tivities and lipid peroxidation inhibition of three fractions were detected by the methods related to Fenton sys-tem, pyrogallol autoxidation, and linoleic acid emulsion system. The reducing power, DPPH radical scavenging activities and alleviation of H2O2-induced oxidative stress on porcine embryonic fibroblast (PEF) cell were also determined. Results EWPH-Ⅲ (MW<3 kDa) accounted for 50.06% of the total amount of EWPHs. The values of hydroxyl radical, superoxide anion and DPPH radical scavenging activities were 52.86%, 35.05%and 78.74% at the concentration of 5 mg/mL, respectively. The percentage of inhibition of lipid peroxidation was 74.57% at the same concentration. The cell survival

以氧化豆油水溶物为试验材料,在草鱼肠道黏膜细胞培养液中加入不同浓度氧化豆油水溶物,研究不同剂量氧化豆油水溶物在不同作用时间下对肠道黏膜细胞生长、细胞形态结构的影响。结果显示:添加111.06—888.48 g/L氧化豆油的水溶物作用6h显著降低细胞活性(P0.05),细胞集落面积减小、细胞形态改变,其中在444.24 g/L氧化豆油的水溶物作用下培养细胞空泡变性,且脂肪滴沉积,线粒体肿胀。在222.12—888.48 g/L氧化豆油的水溶物作用下,3—9h内培养液中LDH活力显著增高(P0.05);各时间点培养液中GSH-PX、SOD、T-AOC均有显著变化。结果表明,在培养液中添加111.06—888.48 g/L氧化豆油的水溶物作用12h内,对草鱼肠道黏膜细胞产生了损伤,表现为抑制细胞生长,改变细胞形态、结构,可能引起细胞膜脂质过氧化,导致膜结构破坏,且作用程度与添加浓度、作用时间相关。研究认为氧化豆油水溶物对草鱼肠道黏膜细胞具有显著性的损伤作用。

The present study investigated the effects of water soluble matter from oxidized soybean oil on the growth, morphology and structure of intestinal epithelial cells (in vitro) from Ctenopharyngodon idella. The water soluble matter was applied at different concentrations for various periods of time. The activity and survival of cells were significant reduced after 6h incubation with culture medium supplemented with water soluble matter in the concentration range of 111.06-888.48 g/L. Furthermore there was an associated decrease in the size of cell colonies as well as a change in cell morphology. When the water soluble matter from oxidized soybean oil reached the concentration of 444.24 g/L, the formation of fat droplets and vesicles in cells could be observed under transmission electron microscope, as well as swelling mitochondria in cells. During 3-9h incubation, LDH activity significantly increased; there was also signi-ficant change in GSH-PX, SOD activity and T-AOC. In conclusion, du

谷胱甘肽过氧化物酶活性的含硒模拟酶试剂（2-SeCD）是一种新型的生物模拟酶，它可以消除机体内的过氧化氢及脂质过氧化物，阻断活性氧自由基对机体的进一步损伤，是生物体内重要的活性氧自由基清除剂。它可防止细胞膜和其它生物组织免受过氧化损伤，并且具有一定的抗炎作用。

The activity of glutathione peroxidase enzyme reagent containing selenium simulation (2 - SeCD) is a new type of biological modeling enzymes w hich could eliminate the of in the hydrogen peroxide and lipid peroxide . At the same time ,it could block the further inju-ry of the active oxygen free radicals in the body ,as an important free radical scavenger of biological active oxygen .It can prevent cell mem-branes and other biological tissues from peroxidation damage ,and has certain anti -inflammatory effects .