蚊虫是传播疟疾、登革热、黄热病、日本脑炎等疾病的重要媒介，嗅觉系统影响着蚊虫的栖息地选择、繁殖、觅食、吸血、趋避及信息传递等行为。嗅觉受体是嗅觉系统的关键成分之一，近年来相关的研究较多。本文简介了蚊虫嗅觉受体蛋白的特征、表达与功能，此外，综述了结构和功能高度保守的Or83 b受体蛋白以及蚊虫驱避剂的研究进展。

Mosquitoes are one of the most important vectors for transmitting diseases such as malaria , dengue fever and yellow fever.In mosquitoes, the olfactory system plays a crucial role in many types of behavior, including nectar feeding, host preference selection and oviposition.Odorant receptors ( ORs ) are comprised of highly variable odorant-binding subunits associated with one conserved co-receptor.They are potential molecular targets for the development of novel mosquito attractants and repellents.ORs have been identified in the malaria mosquito, Anopheles gambiae, and in the yellow fever mosquito, Aedes aegypti.This review enclosed the lately research progress of ORs, including the characteristics of structure, expression regulation and physiological function.Moreover, a highly conserved, broadly expressed olfactory receptor Or83b were summarized in the article.The relationship between OR with the development of mosquito repellents was also involved in the present paper.

蚊虫是重要的医学媒介昆虫，传播着多种虫媒传染病，疟疾、登革热、黄热病、乙型脑炎、基孔肯雅热、西尼罗河热、丝虫病等都因蚊虫的传播而严重危害人类的健康。疫苗和防止蚊虫叮咬仍是预防蚊媒传染病的主要措施。

Mosquitoes are same important medical insects. a lot of infectious diseases, malaria, dengue fever, yellow fever, encephalitis, chikungunya fever, West Nile fever, filariasis, spread by mosquitoes and serious harm to human health. Prevention and control of mosquito-borne diseases is a global public health problem. Protecting from mosquitoes bite and Vaccine is stil a main measures to prevent mosquito-borne infectious diseases.

为了测定球孢白僵菌对不同生境淡色库蚊幼虫的致病力，为白僵菌应用于蚊虫的生物防治提供参考依据，从济宁市唐口镇医院污水（A组）、市郊区养猪场废水沟（B组）、微山湖稻区排水沟（C组）、山东省寄生虫病防治研究所院内水缸（D组）中采集的蚊虫直接用于致病力研究，室内饲养蚊虫作为对照组（E组）。利用白僵菌的孢子悬浮液处理不同栖境的淡色库蚊幼虫，记录幼虫死亡数，计算各处理组幼虫的致死中时LT50。结果显示，经白僵菌处理后，淡色库蚊幼虫均有不同程度的死亡率，试验10 d后，淡色库蚊幼虫的累计死亡率均达95％以上。 A、 B、 C、 D、 E 组幼虫的致死中时 LT50分别为：6.2、6.4、5.7、5.8、3.8 d，野外采集蚊虫比室内饲养蚊虫的死亡高峰期推迟2～3 d。结果表明白僵菌有望应用于蚊虫的生物防治中。

The present study attempts to analyze the virulence of Beauveria bassiana on Culex pipiens pallens from different habitats, such as sewage at Tangkou Town hospital ( A) , polluted piggery water at the outskirts of Jining City ( B) , drainage ditch of paddy field in Weishan Lake ( C) , vats in Shandong Institute of Parasitic Diseases ( D) and laboratory ( E) , and to provide a theoretical basis for biological control of mosquitoes.Spore suspension of B.bassiana was used to process the larvae of Cx.pipiens pallens collected from different habitats.The dead number of larvae was recorded and the median lethal time ( LT50 ) was calculated.The larval mortality was more than 95%10 d after treatment by B.bassiana.The LT50 values of A, B, C, D and E were 6.2 d, 6.4 d, 5.7 d, 5.8 d and 3.8 d, respectively.The LT50 of larvae reared in laboratory was lower.Under laboratory conditions, B.bassiana showed efficient control effect on Cx.pipiens pallens collected from different habitats.The peak value

目的研究河南省太行山区(安阳、鹤壁段)的蚊虫种类多样性。方法利用人工小时法对调查点的牲畜棚和村民居住房等蚊虫栖息场所进行成蚊的采集调查,计算密度指数;同时对周围河流、坑洼积水处的蚊蚴进行虫种的观察和鉴别。结果调查共捕获成蚊748只,蚊蚴319只,分属于4属9种,分别为中华按蚊、潘氏按蚊、淡色库蚊、三带喙库蚊、伪杂鳞库蚊、中华库蚊、白纹伊蚊、刺扰伊蚊和骚扰阿蚊。安阳段的密度指数高于鹤壁段。结论太行山区(安阳、鹤壁段)的优势蚊种为骚扰阿蚊,其次为伪杂鳞库蚊和刺扰伊蚊,所占构成比分别为57.14%、16.75%和9.85%。两地区间密度指数的差异主要由于调查期间降水量差异引起的,说明蚊虫的生存与气候因素和环境因素等生态系统相关,调查结果也会受到各种相关因素的影响。

Objective To research species diversity of mosquitoes in Taihang Mountain area(Anyang,Hebi section)of Henan Province. Method Adult mosquitoes in livestock sheds and villagers living room were collected by labor hour method. Larvae in the nearby rivers and potholes water were observed and identified at the same time. Results 748 adult mosquitoes and 319 larvae were collected in this research,these mosquitoes belong to 4 genera and 9 species, such as An. sinensis,An. pattoni,Cx. pipiens pallens,Cx. tritaeniorhynchus,Cx. Pseudovishnui,Cx. sinensis,Ae. albopictus,Ae. vexans and Ar. Subalbatus. The density index of Anyang section is higher than Hebi section. Conclu-sion The advantage mosquitoes in Taihang Mountain area(Anyang,Hebi section)is Ar. Subalbatus,Cx. Pseudovish-nui and Ar. subalbatus were followed. The ratios were 57.14%,16.75%and 9.85%. The density index difference was coursed by precipitation difference of tow sections. This phenomenon illustrated that the survival of the mosqui

目的：分析浙江省仙居县媒介蚊虫中流行性乙型脑炎（乙脑）病毒（JEV）基因型别和健康人群乙脑抗体水平。方法2012-2013年在浙江省仙居乙脑监测点采集蚊虫，分离JEV，测定其E基因核苷酸序列，并进行同源性与进化分析；对监测点的642名健康人群，在乙脑流行季节前后，分别采集双份血清，共计样本1263份，检测JEV中和抗体。结果采集11650只蚊虫标本，分离25株JEV，同源性分析显示，2012-2013年分离到的JEV与浙江省1982-2010年JEV之间E基因核苷酸同源性为87.8%～99.7%，与乙脑疫苗株SA14-14-2之间核苷酸同源性为87.7%～88.0%。E基因进化分析显示，2012-2013年JEV属于基因Ⅰ型，E基因编码病毒毒力和抗原表位的位点未发生改变。健康人群JEV中和抗体阳性率为31.5%～42.0%，抗体几何平均滴度（GMT）为1∶2.56～1∶3.53，流行前后人群抗体阳性率（χ2≤1.76，P＞0.05）和GMT（u≤0.64，P＞0.5）差异均无统计学意义。结论2012-2013年浙江省仙居县蚊媒中携带基因Ⅰ型JEV，健康人群JEV中和抗体阳性率≤42.0%，流行前后抗体阳性率和中和GMT均无明显变化。

Objective To understand the genotypic characteristics and the neutralizing antibody levels of Japanese encephalitis virus(JEV)and Japanese encephalitis(JE)in both vector mosquitoes and in healthy people of Zhejiang province. Methods Virus was isolated from mosquitos sampled from the Monitoring Stations located in Xianju county during 2012 to 2013. Phylogenetic and homological studies were carried out on the E gene. A total of 1 263 blood specimens from 642 healthy people were collected before and after the seasons of JE epidemics. JEV neutralizing antibody was detected by the micro-neutralization test. Results Twenty-five JEV strains were isolated from a total of 11 650 mosquitoes. The identity of nucleotide appeared as 87.8%-99.7% both from 2012 to 2013 and from 1982 to 2010 while as 87.7%-88.0%with vaccine strain SA14-14-2,in Zhejiang. The phylogeny tree of E gene indicated that the newly isolated virus belonged to genotypeⅠ but no mutation of amino acid sequence coding co