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双语推荐：Bzip2

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近年来，Bzip2压缩算法凭借其在压缩率方面的优势，得到了越来越多的应用，Bzip2的核心算法是Burrows-Wheeler变换(BWT), BWT能有效的将数据中相同的字符聚集到一起，为进一步压缩创造条件。在硬件实现 BWT 时，常用的基于后缀排序的算法能有效克服 BWT 消耗存储资源大的问题，该文对基于后缀排序实现BWT的方法进行了详细分析，并且在此基础上提出了一种快速实现BWT的方法后缀段算法。仿真结果表明后缀段算法在处理速度上比传统的基于后缀排序的算法有很大的提高。
Bzip2, a lossless compression algorithm, is widely used in recent years because of its high compression ratio. Burrows-Wheeler Transform (BWT) is the key factor in Bzip2. This method can gather the same symbols together. The traditional methods which are based on suffix sorting used in implement of BWT in hardware can solve the problem of memory consumption effectively. Detail analysis of BWT algorithm based on suffix sorting is given and a new methodSuffix segment method is presented in this paper. Experimental results show that the proposed method can much decrease BWT time consumption without increasing memory consumption much.

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转录因子通过调控下游基因的表达来缓冲各种环境压力反应。其中AP2／EREBPL参与植物的细胞周期、生长发育、生物胁迫和非生物胁迫相关的基因的表达调控；MYB参与植物的细胞周期、细胞死亡、新陈代谢等响应；bZIP基因参与植物种子贮藏相关的基因表达，控制光和发育的发生和器官形态建成等；NAC基因参与了植物激素信号传导和生长素通路。这些转录因子通过调控一系列基因的表达增强植物忍耐逆境胁迫能力。
Transcription factors can response to various forms of environmental stress by regulating downstream gene expres-sion. Among of the transcription factors, AP2/EREBPL involves in plant cell cycle, growth, biological stress and related gene expression regulation under abiology stress. MYB involves in cell cycle, cell death and response metabolism in plant. BZIP are connected with seed storage gene expression, controlling the occurrence of the light and development and the formation organ in plant. NAC genes take in plant hormone signal transduction and auxin pathways. These transcription factors en-hance the plant’stress tolerance ability by regulating the expression of a series of genes.

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低温是严重影响农业生产的重要因素之一,尤其是在高海拔的冷凉地区。近年来随着抗寒基因工程的发展,有关植物抗寒性的研究得到了很大的进展。本研究从抗寒相关的功能基因和调控基因两方面入手,归纳了就近几年国内外关于植物的抗寒相关功能基因（冷诱导基因、脂肪酸去饱和代谢关键酶基因和抗氧化酶基因等）和调控基因（AP2/EREBP、NAC、MYB、WRKY和bZIP转录因子）并提出了展望,以期为人们进一步认识和利用植物抗寒相关基因提供参考,为植物抗寒机理的研究和植物抗寒育种提供依据,期望随着研究的深入能够培育出多抗寒性植物新品种。
Low temperature was one of the important environment factors affecting agriculture, especially in higher altitudes and cool areas. In the recent years, with the plant cold-resistant genetic engineering development, great progress was obtained in researches of cold resistance. This paper gave a general statement about the recent development of plant cold-resistant genes from cold regulated gene, structural genes (including fatty acid desaturation metabolism enzyme gene and antioxidative enzyme gene etc.) and regulatory genes (AP2/EREBP, NAC, MYB, WRKY and bZIP transcription factors), aiming to provide some useful information and ideas to researchers who work on plant breeding and the mechanism of cold-resistance. As research continues, we could develop new multiple resistance plant breeds.

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背景 视神经蛋白(OPTN)基因是青光眼和肌萎缩性脊髓侧索硬化的致病基因,在眼部视网膜组织中的表达最多.我们先前的研究分离出与OPTN相互作用的蛋白质,确定其中之一为视网膜色素变性的致病基因——碱性亮氨酸拉链(bZIP)转录因子,即神经视网膜亮氨酸(NRL)拉链,并证实OPTN和NRL两蛋白间存在相互作用. 目的 确定与NRL相互作用所必需的OPTN蛋白结合区域. 方法 构建一系列带FLAG标签的OPTN部分片段缺失质粒,分别与带血凝素标记的NRL(HA-NRL)共转染HeLaS3细胞.用抗HA和抗FLAG标签抗体分别对细胞质和细胞核提取物进行免疫共沉淀和Western blot检测,分析两蛋白质间的相互结合方式,以确定NRL与OPTN蛋白的结合区域.结果 在OPTN的第一、第二和第三缺失区域质粒转染细胞的细胞核组分中均检测出血凝素标记的NRL(HA-NRL)免疫共沉淀条带,但在第四缺失区域质粒中不产生此HA-NRL条带.从所有缺失质粒及全长OPTN质粒转染细胞的细胞质组分中均未观察到NRL条带.结论 本研究进一步证实OPTN和NRL在HeLaS3细胞核内的相互作用,并明确其蛋白结合区域.在共表达NRL和OPTN的HeLaS3细胞中,Flag-OPTN可与HA-NRL相互结合,且OPTN的尾端区域(423-577)是结合NRL所必需的.
Background Gene encoding optineurin (OPTN) is a causative gene for glaucoma and amyotrophic lateral sclerosis,with a more expression in retina.Our previous study isolated OPTN-interacting proteins and identified that the gene encode the basic leucine zipper (bZIP) transcription factor neural retina leucine (NRL) zipper,a causative gene for retinitis pigmentosa,and further study demonstrated the interaction between OPTN and NRL proteins in nuclei of cultured HeLaS3 cells.Objective This study was to determine the protein binding site of OPTN necessary for NRL binding.Methods A deletion series of OPTN-expression plasmids were constructed and co-expressed with hemagglutinin (HA)-tagged NRL in HeLaS3 cells,respectively.The cytoplasmic and nuclear fractions were used to perform co-immunoprecipitate (CoIP) and Western blot with anti-tag antibodies.Results In the nuclear fractions of cells transfected with the del1 st,del2nd or del3rd plasmid,a band of coimmunoprecipitated HA-labelled NRL (HA-

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