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双语推荐：CD19

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目的检测系统性红斑狼疮（ SLE）患者和健康者外周血CD19＋ B细胞中B淋巴细胞诱导成熟蛋白-1（Blimp-1） mRNA的表达,并探讨Blimp-1在SLE的临床意义。方法应用实时荧光定量PCR技术检测40例SLE患者和20例健康者外周血CD19＋ B细胞中Blimp-1 mRNA的表达水平,应用流式细胞术检测外周血中CD19-CD138＋浆细胞/CD19＋B细胞比例、CD19＋CD27＋记忆性B细胞/CD19＋ B细胞比例,分析SLE患者Blimp-1表达与B细胞亚群及临床相关指标的相关性。结果① SLE 组 CD19＋ B 细胞中 Blimp-1 mRNA的表达水平高于正常对照组（ P〈0.05）、活动组高于稳定组（P〈0.05）；② SLE组CD19-CD138＋浆细胞/CD19＋B细胞比例显著高于正常对照组（ P〈0.01）、活动组高于稳定组（ P 〈0.05）；SLE 组与正常对照组外周血中 CD19＋CD27-记忆性B细胞/CD19＋ B细胞比例差异无统计学意义（ P〉0.05）；③SLE患者Blimp-1表达与CD19-CD138＋浆细胞/CD19＋ B细胞比例呈正相关性（rs =0.336,P=0.034）,与CD19＋CD27＋记忆性 B细胞/CD19＋ B细胞比例无显著相关性（rs =-0.271,P=0.116）,与抗ds-DNA抗体、免疫球蛋白IgG呈正相关性（ rs =0.493,P =0.006；rs =0.527,P=0.004）,与抗核小体抗体、抗 C1q 抗体呈正相关
Objective To detect the mRNA expression of B lymphocyte induced maturation protein-1 ( Blimp-1 ) in peripheral blood CD19 + B cells from patients with systemic lupus erythromatosus( SLE) and healthy people,and ex-plore its clinical significance. Methods Expression level of Blimp-1 mRNA in peripheral blood CD19 + B cells was determined by real-time PCR in 40 SLE patients and 20 healthy controls. The ratio of CD19 -CD138 + plasma cells and CD19 + B cells and the ratio of CD19 +CD27 -memory B cells and CD19 + Bcells were studied by flow cytometry in peripheral blood from two groups. Results ① The expression level of Blimp-1 mRNA in peripheral blood CD19 +B cells from SLE patients group was higher than that in normal control group(P 0.05 ) .③The expression level of Blimp-1 was positively correlated with the CD19 -CD138 + plasma cells/CD19 + B cells ratio(rs=0.336,P=0.034),not correlated with the CD19 +CD27 -memory B cells/CD19 + B cells ratio(rs=-0.271,P=0.116),positively corre

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目的：探讨儿童溃疡性结肠炎(Ulcerative Colitis)患者外周血CD3-CD19+的表达及其意义。方法：采用流式细胞分析法，检测20例UC患者和20例正常(对照组)外周血CD3-CD19+的比例。结果：US组CD3-CD19+的表达明显高于正常对照组(P＜0.001)，UC组与正常对照组比较差异有统计学意义(P＜0.05)。结论：溃疡性结肠炎患者外周血CD3-CD19+的表达值较正常对照明显升高，提示CD3-CD19+可能在UC的病理机制中发挥作用。
Objective:To explore the expression and significance of CD3-CD19+in peripheral blood of children with ulcerative colitis(UC).Methods:Using flow cytometry,peripheral blood CD3-CD19+ ratio of 20 patients with UC and 20 normal people(the control group) were detected.Results:In ulcerative colitis group,the expression of CD3-CD19+was significantly higher than that in the normal control group(P<0.001).Compared the group of UC patients with the normal control group,the difference was statistically significant(P<0.05).Conclusion:In patients with ulcerative colitis,compared with the normal control group,the expression of peripheral blood CD3-CD19+ value is increased significantly.The results suggests that CD3-CD19+ may play a role in the pathogenesis of UC.

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目的研究抗体毒素偶联新型药物anti-CD19(Fab)-LDM体内外靶向CD19+B淋巴瘤细胞的能力。方法制备Cy5标记的anti-CD19(Fab)-LDP(LDP为LDM辅基蛋白),体外流式细胞仪分析其与人淋巴瘤Raji细胞表面CD19抗原的结合能力;体内建立裸鼠淋巴瘤皮下移植瘤模型,应用活体动物成像系统观察Cy5标记的anti-CD19(Fab)-LDP在荷瘤小鼠体内的靶向分布。结果 Cy5标记的anti-CD19(Fab)-LDP能有效与淋巴瘤Raji细胞膜表面的CD19抗原结合;成功建立人淋巴瘤Raji细胞皮下移植瘤模型,体内活体成像结果表明,融合蛋白anti-CD19(Fab)-LDP可靶向定位到肿瘤部位。结论体内外实验证实anti-CD19(Fab)-LDP能很好的靶向CD19+的淋巴瘤细胞,此抗体毒素偶联药物anti-CD19(Fab)-LDM有希望成为治疗恶性淋巴瘤的新型药物。
Aim To study targeting capability of anti-CD19 (Fab)-LDMto CD19 +B lymphoma cells in vi-vo and in vitro.Methods Flow cytometry was em-ployed to determine the affinity of Cy5 labeled anti-CD19 (Fab)-LDP to human lymphoma Raji cells.And the optical imaging system was used to analyze the dis-tribution of Cy5-anti-CD19 (Fab )-LDP in lymphoma-transplanted xenograft nude mice in vivo.Results The results of flow cytometry demonstrated that Cy5-an-ti-CD19(Fab)-LDP had remarkable affinity with lym-phoma Raji cells;Raji lymphoma xenograft model wasestablished successfully in nude mice and in vivo fluo-rescence imaging analysis indicated that the antibody-drug conjugates could specially be localized in the tar-get tumor.Conclusion The experiments in vivo and vitro confirm that anti-CD19 (Fab)-LDP has remarka-ble affinity to targeting CD19 +lymphoma cells,and the antibody drugs anti-CD19 (Fab )-LDP have the probability to be new drugs for the treatment of malig-nant lymphoma.

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目的：探讨流式细胞术检测浆细胞骨髓瘤的非典型免疫表型特征及其临床意义。方法采用病例对照性研究，收集中山大学孙逸仙纪念医院和暨南大学附属第一医院2011年1月至2013年12月住院或门诊非典型免疫表型特征浆细胞骨髓瘤患者48例。此48例非典型免疫表型特征浆细胞骨髓瘤患者为研究对象，42例免疫表型、骨髓形态学及血清免疫固定电泳结果均正常的病例作为对照组，运用流式细胞仪检测其特异性CD抗原标志，并根据非典型免疫表型特征浆细胞骨髓瘤患者流式检测结果，按照 CD38、CD138、CD19、CD56的表达情况将其分为 CD38＋/CD138＋/CD19＋/CD56＋、CD38＋/CD138＋/CD19＋/CD56-、CD38＋/CD138＋/CD19-/CD56-3组，进一步比较这3组胞浆内免疫球蛋白轻链（ cKappa、cLambda）表达情况，并结合骨髓形态学及血清免疫固定电泳结果进行分析比较。样本率的比较采用χ2检验。结果48例具非典型免疫表型特征浆细胞骨髓瘤患者中，抗原表达率分别为：CD4529.17％、CD38100％、CD138100％、CD1995.83％、CD5643.75％、cKappa 43.75％和 cLambda 56.25％。3组患者的克隆性免疫球蛋白表达率分别为43.75％、52.08％和4.17％；骨髓形态学及血清免疫固定电泳阳性率分别为57.14％、80％、100
Objective To investigate the characteristics and significance of atypical flow cytometric immunophenotype in plasma cell myeloma.Methods Using case-control study , 48 cases with atypical immunophenotype of plasma cell myeloma and 42 cases as control group were studied by flow cytometry , the former cases were classified into three groups according to the expression of CD 38, CD138, CD19, CD56 :CD38 +/CD138 +/CD19 +/CD56 +, CD38 +/CD138 +/CD19 +/CD56 -, CD38 +/CD138 +/CD19 -/CD56 -.And compare the three groups with intracellular immunoglobulin light chain (cKappa, cLambda) , bone marrow morphology and immunofixation.The positive rate was compared with chi-square test.Results Bone marrow plasma cells showed expression of particular antigens in the following proportion of the 48 cases:CD45 29.17%, CD38 100%, CD138 100%, CD19 95.83%, CD56 43.75%, cKappa 43.75%and cLambda 56.25%.And in the three groups , the expression of monoclonal immunoglobulin were43.75%, 52.08%and 4.17%, whic

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目的 检测免疫相关性全血细胞减少症(IRP)患者外周血CD3+、CD3+CD4+、CD3+CD8+、CD19+淋巴细胞及骨髓CD34+细胞端粒长度变化并分析其与病情严重程度的关系.方法 以40例初治IRP患者为研究对象,应用免疫磁珠方法分选外周血CD3+、CD3+CD4+、CD3+CD8+T淋巴细胞,CD19+B淋巴细胞及骨髓CD34+细胞,以流式细胞术-荧光原位杂交技术检测端粒相对长度,并分析端粒长度与IRP患者病情严重程度的相关性.结果 初治IRP患者外周血CD3+、CD3+CD4+、CD3+CD8+T淋巴细胞端粒相对长度分别为(27.754±16.323)％、(7.526±3.745)％、(25.854±14.789)％,与健康志愿者的(54.555± 19.782)％、(12.096±2.805)％、(38.367±4.626)％比较明显缩短(P＜0.05);IRP患者外周血CD19+B淋巴细胞端粒相对长度为(22.136±16.142)％,与健康志愿者的(42.846±16.353)％比较亦明显缩短(P＜0.01);IRP患者骨髓CD34+细胞端粒相对长度为(22.528±21.601)％,与健康志愿者的(23.936±19.822)％比较差异无统计学意义(P＞0.05).B淋巴细胞端粒相对长度与外周血白细胞计数呈正相关(r=0.706,P=0.015),而与临床表现积分呈负相关(r=-0.613,P=0.045),与治疗效果呈正相关(r=0.775,P=0.005).结论 初治IRP患者存在CD3+、CD3+CD4+、
Objective To investigate the changes of relative telomere length (RTL) of peripheral blood (PB) CD3+,CD3+CD4+,CD3+CD8+T lymphocytes,CD19+B lymphocytes and bone marrow (BM) CD34 + cells and its association with disease severity in untreated patients with immuno-related pancytopenia (IRP).Methods The PB CD3 +,CD3 + CD4+,CD3 + CD8 +T lymphocytes,CD19 +B lymphocytes,and BM CD34+ cells were purified by magnetic activated cell sorting (MACS),and RTL were measured with flow-fluorescence in situ hybridization (FLOW-FISH).Results The RTL of CD3+,CD3+CD4+,and CD3+CD8+T lymphocytes in untreated IRP patients were (27.754± 16.323)％,(7.526± 3.745)％ and (25.854± 14.789)％,respectivly,which were significantly shorter than those in healthy controls (54.555± 19.782)％,(12.096±2.805)％,and (38.367±4.626)％ (P＜0.05).The RTL of CD19+ lymphocytes in untreated IRP patients was (22.136± 16.142)％,which was significantly shorter than that in healthy controls (42.846± 16.353)％ (P＜0.01).Th

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观察门脉高压症患者脾切除术后免疫功能的改变,探讨门脉高压症患者术后免疫功能改变的原因。方法:用流式细胞仪对30例门脉高压症患者分别测定术前及术后一周静脉血淋巴细胞亚群(CD3+、CD4+、CD8+、CD19+及CD56+)的数值,并与健康体检者的淋巴细胞亚群数值做比较。结果:健康对照组与门脉高压症术前组比较CD3+、CD4+、CD8+、CD19+及CD56+经统计学比较差异有统计学意义(P0.05)。门脉高压症术前组与门脉高压症术后一周组比较CD3+、CD4+、CD8+、CD19+及CD56+经统计学比较差异有统计学意义(P0.05)。结论:门脉高压症患者存在免疫功能低下状态,可能与脾功能亢进有关。
Objective:To obs erve the c hanges of immune function in portal hypertension patients after splenectomy and to discuss the reasons for the changes. Methods: Lymphocyte subsets (CD3+,CD4+,CD8+,CD19+,CD56+) of peripheral blood in 30 portal hypertension patients with preoperative and postoperative a week were measured by flow cytometer . Then compared with healthy physical examination people . Result: The CD3+、CD4+、CD8+、CD19+ and CD56+in healthy physical examination group compared with preoperative group were significant difference (P<0.05). The CD3+、CD4+、CD8+、CD19+ and CD56+ in preoperative group compared with postoperative a week group were significant difference(P<0.05).Conclusion: The immune function of Portal hypertension patients was disorder,which may be associated with the hypersplenism.

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目的探讨原发性高血压（EH）患者N末端脑钠肽前体（NT-pro BNP）及淋巴细胞亚群等指标变化情况。方法 328例高血压确诊患者按照分级标准进行分组,其中EH1级组115例,EH2级组109例,EH3级组104例;以120例同期健康体检者作为对照组。采用电化学发光免疫分析测定NT-pro BNP水平和流式细胞仪分析CD3^＋、CD4^＋、CD8^＋、CD19^＋水平,组间进行比较。结果高血压组血清中NT-pro BNP、CD4^＋/CD8^＋、CD19^＋水平较健康对照组明显升高（P〈0.05）,其中EH3级组升高最显著;CD3^＋、CD4^＋、CD8^＋细胞明显低于正常对照组（P〈0.05）,EH3级组降低最显著。NT-pro BNP水平与CD3^＋、CD4^＋、CD8^＋等指标均呈显著负相关（r=-0.951,r=-0.973,r=-0.955;P均〈0.01）,与CD19^＋细胞（B细胞）、CD4^＋/CD8^＋呈显著正相关（r=0.934,r=0.98;P均〈0.01）。结论 EH患者的血压的临床分级与NT-pro BNP、CD3^＋、CD4^＋、CD8^＋、CD19^＋的水平密切相关,并可能参与EH的病理生理过程。
Objective To study the change of N-terminal pro-brain natriuretic peptide and lymphocyte sub-population in essential hypertensive patients. Methods Totally 328 hypertensive patients were divided into three groups according to the standard of classification:grade 1 hypertensive (115 patients), grade 2 hypertensive (109 pa-tients) and grade 3 hypertensive (104 patients). As the same time 120 normal controls were enrolled as control group. CD3+、CD4+、CD8+、CD19+level and serum NT-proBNP concentration were measured by flow cytometry and electroche-miluminescence immunoassay (ECLIA) . Results NT-ptoBNP, CD4/CD8 and CD19+ levels in 328 patients with EH were significantly higher than those in the controls (P r=-0.973, r=-0.955;P<0.01), and was positively correlated with CD19+ and CD4+/CD8+ (r=0.934, r=0.98; P<0.01). Conclusion NT-proBNP, CD3+, CD4+, CD8+ and CD19+were closely correlated with EH stage and probably in-volved in the pathophysiological process of EH.

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目的在比较健康人、结核潜伏感染者、活动性肺结核患者B细胞及亚群变化的基础上了解活动性肺结核患者治疗过程中B细胞百分比及B细胞亚群的分布情况。方法根据B细胞表面CD19/CD5/CD1d标识将B细胞分为四群。利用流式细胞术比较活动性肺结核患者治疗前、治疗3个月、治疗6个月、治疗9个月时外周血B细胞百分比及B细胞分群表达的差异。结果结核患者治疗6个月时外周血B细胞较治疗3个月时有所增加(P0.01)。结核患者治疗3个月时外周血中CD19+CD5-CD1d+和CD19+CD5+CD1d+B细胞百分比最高,并随治疗时间的延长而逐渐下降(P0.01)。结核患者治疗3个月时CD19+CD5-CD1d-B细胞百分比较治疗前下降(P0.01),但随治疗时间的延长而逐渐上升(P0.01)。结核患者外周血CD19+CD5+CD1d-B细胞百分比在治疗后逐渐上升,结果有统计学差异(P0.01)。结论肺结核患者外周血中紊乱的B细胞亚群在治疗过程中,逐渐恢复正常,提示B细胞亚群在结核的发病中起重要作用。
Objective To explore the dynamic variation of B cell subsets in the patients with tuberculosis (TB).Methods Ac-cording to expression of CD19/CD5/CD1d on the surface of cells,B cells were divided into four groups.B cell subsets in peripheral blood of TB patients at different stages were analyzed by using flow cytomety.Results The frequency of B cells in TB patients af-ter treatment of 6 months was higher than treatment of 3 months (P<0.01).Proportion of CD19+CD5-CD1d+B cells in TB pa-tients after treatment of 3 months was the highest,but gradually declined after treatment of 6 months and 9 months (P<0.01). Proportion of CD19+CD5+CD1d+B cells in TB patients after treatment of 3 months was the highest,but gradually declined after treatment of 6 month and 9 months (P<0.01).Proportion of CD19+CD5-CD1d-B cells in TB patients after treatment of 3 months was lower than before treatment (P<0.01),but gradually increased after treatment of 6 months and 9 months (P<0.01). Proportion of CD19+CD5+CD

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目的探讨CD3-CD19+在Graves病（GD）各阶段病程中的含量变化及临床意义。方法采用流式细胞术检测GD未治疗组（初发组）、甲状腺缓解组、完全缓解组、复发组及正常对照组CD3-CD19+比值，将各组结果进行统计学分析。结果初发组结果明显高于正常对照组、缓解组、完全缓解组及复发组，差异有统计学意义（P分别为0.002、0.023、0.002、0.026），其余各组别比较虽有差异，但没有统计学意义。结论 CD3-CD19+的结果与GD整个病程的免疫反应相一致，体液免疫反应在GD病程中都起到很重要的作用，CD3-CD19+结果对GD各阶段病程的诊断、治疗及预后有临床意义。
Objective To investigate the changes in the contents of CD3-CD19+at various stages of Graves disease (GD) and its clinical significance. Methods The ratio of CD3-CD19+ was detected by flow cytometry in patients with Graves disease, including the untreated group, the remission group, the complete remission group, the relapsed group and the normal control group. Results The number of CD3-CD19+in the untreated group were apparently higher than that of the normal control group, the remission group, the complete remission group and the relapsed group, which had statistical significance respectively (P=0.002, 0.023, 0.002, 0.026). But there were no statistical significance between the other Graves disease groups. Conclusion The results of CD3-CD19+were consistent with the immune reaction throughout the GD. Since the humoral immune played an important role in the course of GD, the CD3-CD19+had an important clinical significance in various stages of diagnosis, treatment and prognosis of GD.

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目的研究不同免疫干细胞表型的急性白血病患者的治疗效果及预后情况。方法将本院2010年1月至2013年12月收治的78例急性白血病患者作为研究对象,检测其免疫干细胞表型,并记录治疗情况。结果急性白血病免疫干细胞表型CD34+的患者比例最高(71.39%),其次为CD33+、CD64+患者;免疫干细胞CD33、CD64、CD34、CD19呈阳性的患者治愈率均低于其表型呈阴性的患者,差异具有显著性(t=11.1682,9.5098,10.6997,7.2341;P=0.0008,0.0020,0.0011,0.0072);免疫干细胞表型CD34+CD19+患者的治愈率高于CD34+CD19-的患者;而CD34+CD64+患者的治愈率低于CD34+CD64-的患者,差异具有显著性(t=6.9115,6.3096;P=0.0086,0.0120)。结论不同免疫干细胞表型的急性白血病患者的治愈率不同,免疫干细胞表型与急性白血病患者的治疗效果、预后密切相关。
Objective Patients with acute leukemia treatment effect and prognosis of different phenotypes of immune. Method 78 cases of acute leukemia patients in our hospital from January 2010 to December 2013 as the research object, its immune stemcell phenotype was detected, and record the treatment situation. Result Immune in patients with acute leukemia stem cell phenotype in patients with CD34+ the highest proportion (71.39%), followed by CD33+, CD64+;CD33, CD64 in patients with immune cells, CD34, CD19 positive patients, the cure rate was lower than the phenotypic negative patients, the difference was signiifcant (t=11.1682, 9.5098, 10.6997, 7.2341;P=0.0008, 0.0020, 0.0011, 0.0072);cure the patients immune stem cellphenotype of CD34+CD19+was higher than that of the CD34+CD19-patients;and the healing rate of the patients with CD34+CD64+than patients with CD34+CD64-, and the difference was signiifcant (t = 6.9115, 6.3096; P = 0.0086, 0.0120). Conclusion The cure rate of patients with acute le

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