目的探讨单核细胞趋化蛋白-1（MCP—1）在神经干细胞体内迁移中的作用。方法雄性Sprague—Dawley大鼠制备脑皮质微量注射脂多糖脑损伤大鼠模型，于造模后3d、5d、7d灌注取脑，采用免疫组织化学方法检测MCP．1和nestin表达。结果造模后，MCP．1表达增高，5d时达到高峰；7d时，可在损伤区发现nestin阳性细胞。结论MCP．1可能趋化内源性神经干细胞向脑损伤区迁移。

Objective To investigate the effects of monocyte chemoattractant protein-1 (MCP-1) on the migration of neural stem cells in vivo. Methods The brain injured rat model was established with cortical micro-injection of lipopolysaccharide in Sprague-Dawley rats. The tissue of brain was immunohistochemistrically stained to detect the expression of MCP-1 and nestin 3 d, 5 d, and 7 d after modeling. Results The expression of MCP-1 increased, and reached a peak 5 d after modeling. The nestin-positive cells was found in the lipopolysaccharide in-jected area 7 d after modeling. Conclusion MCP-1 may induce the endogenous neural stem cells migrate to lipopolysaccharide injection ar-ea in vivo.

目的观察后肢制动对大鼠比目鱼肌神经传导功能的影响。方法 24只健康雌性Sprague-Dawley大鼠随机分为正常对照组、制动7 d组和制动14 d组,每组8只。采用石膏固定建立后肢制动模型,用电生理技术记录背根细束的传导速度和M波潜伏期。结果与正常对照组比较,制动7 d后,大鼠比目鱼肌肌梭Ⅰ类传入神经纤维的传导速度减慢(P0.05);制动14 d后,M波潜伏期延长(P0.05)。结论制动条件下神经传导功能降低,而且传入神经纤维的功能改变要早于、重于运动神经纤维。

Objective To observe the alterations in soleus nerve conduction velocities induced by hindlimb immobilization. Methods 24 healthy Sprague-Dawley rats were randomly divided into control group, immobilization 7 days group and immobilization 14 days group with 8 cases in each group. The plaster cast was fixed as the method to induce a hindllimb immobilization model in rats. The afferent thin fi-bers conduction velocity and the latency of M wave were observed with electrophysiological technique. Results 7 days after immobilization, the conduction velocity of muscle spindle primary afferent fiber decreased (P<0.05), the latency of M wave significantly prolonged 14 days after immobilization (P<0.05). Conclusion The soleus nerve conduction velocities decrease significantly following hindlimb immobilization and the alterations in afferent fibers are more serious than that in efferent fibers.

目的观察莫诺苷对二磷酸腺苷(ADP)诱导的局灶性脑缺血再灌注大鼠血小板聚集指数的影响.方法用线栓法制备大鼠局灶性脑缺血再灌注模型.将48只Sprague-Dawley大鼠随机分为假手术组,模型组,莫诺苷低、中、高剂量组(30 mg/kg、90 mg/kg、270 mg/kg)和阿司匹林组(10 mg/kg).利用透光比浊法检测大鼠血小板聚集率.结果与模型组相比,高剂量莫诺苷组血小板最大聚集率显著降低(P<0.001).结论莫诺苷具有抑制血小板聚集的作用.

@@@@Objective To explore the effects of morroniside on platelet aggregation induced by adenosine diphosphate (ADP) in focal ce-rebral ischemia/reperfusion in rats. Methods 48 Sprague-Dawley rats were randomly divided into sham group, model group, morroniside dose groups (30 mg/kg, 90 mg/kg, 270 mg/kg) and acetyl salicilic acid (ASA) group (10 mg/kg). The model of middle cerebral artery occlu-sion (MCAO) was established in all rats except the sham group. Born's turbidimetry was used to measure platelet aggregation rate in rats of MCAO model (in vivo). Results Compared with the model group, the platelet aggregation decreased significantly in the morroniside high dose group (P<0.001). Conclusion Morroniside has the effect of anti-platelet aggregation in focal cerebral ischemia/reperfusion in rats.

目的探讨骨髓基质细胞(BMSCs)经侧脑室植入脑缺血大鼠后海马齿状回突触形成及大鼠记忆能力的变化。方法大鼠BMSCs培养3代。60只新生Sprague-Dawley大鼠分为假手术组、模型组、细胞组各20只,后两组结扎左侧颈总动脉,细胞组于7 d后经侧脑室植入BMSCs。8周后行Morris水迷宫测试。测试后取脑组织,免疫组织化学染色检测海马齿状回处突触素表达。结果模型组探查训练(T1)和对位探查训练(T2)时间均较假手术组缩短(P0.05);细胞组T1和T2分别较模型组延长(P0.05)。模型组大鼠海马突触素阳性细胞积分光密度值(IOD)较假手术组减小(P0.05),细胞组IOD较模型组增加(P0.05)。结论 BMSCs经侧脑室植入可促进脑缺血大鼠海马齿状回突触形成,并改善其记忆功能。

Objective To observe the synapse formation in hippocampal dentate gyrus, and the memory ability after transplanting bone marrow stromal cells (BMSCs) into lateral ventricle of ischemic brain injury rats. Methods BMSCs from femur of a Sprague-Dawley rat were cultured for 3 generations in vitro. 60 newborn Sprague-Dawley rats were divided into sham group (n=20), model group (n=20) and BMSCs group (n=20). The latter 2 groups were ligated the left common carotid artery, and the BMSCs group were injected BMSCs into the lateral ventricle 7 days after ligation. They were tested with Morris''s water maze 8 weeks later, and then, the brains were immunohistochemi-cal staining to detect synaptophysin (SY). Results The time of probe trial of acquisition phase (T1) and reveral phase (T2) decreased in the model group compared with the sham group (P<0.05), and increased in the BMSCs group compared with the model group (P<0.05). The in-tegral optical density (IOD) of SY positive cells decrea

目的：研究探索学习环境对局灶性脑梗死大鼠学习记忆能力及缺血侧海马区微血管密度的影响。方法80只Sprague-Dawley大鼠开颅电凝右侧大脑中动脉，随机分为标准环境组(SE组, n=40)和探索学习环境组(LE组, n=40)。分别于术后7 d、28 d进行Morris水迷宫测试；术后1 d、3 d、7 d、14 d、28 d，CD34免疫组织化学法测定海马区微血管密度。结果 LE组Mor-ris水迷宫测试成绩显著优于SE组(P<0.001)。术后7 d起，LE组缺血侧海马区微血管密度高于SE组(P<0.05)。结论探索学习环境可促进脑梗死大鼠微血管新生，有利于学习记忆功能恢复。

Objective To study the effects of learning environment on learning memory and microvessel density in hippocampus of rats after cerebral infarction. Methods 80 Sprague-Dawley rats were coagulated right middle cerebral arteries electrically, and randomly divided into learning envionment (LE) and standard environment (SE) groups. They were tested with Morris Water Maze 7 d and 28 d after opera-tion. The microvessel density in hippocampus was measured with CD34 immunohistochemstry 1, 3, 7, 14 and 28 days after operation. Re-sults The achievement of Morris Water Maze was significantly better in the LE group than in the SE group (P<0.001), while the microvessel density in the hippocampus was more since 7 days after operation (P<0.05). Conclusion The learning environment can promote the angio-genesis and improve the learning and memory function recovery.