背景与目的转录因子21(transcription factor 21,TCF21)是新近发现的抑癌基因,其在多种肿瘤中具有抑癌功能,本研究旨在探讨TCF21基因对人肺癌A549细胞增殖、迁移和凋亡的影响。方法利用慢病毒转染技术在肺癌A549细胞中高表达TCF21基因,以荧光定量PCR、Western blot分析目的基因的表达,并采用Transwell、MTT法、流式细胞术检测TCF21高表达对A549迁移、增殖、凋亡的影响。结果成功在肺癌细胞株A549中高表达TCF21,且高表达TCF21后A549的细胞生长和迁移能力受抑制、凋亡率增高。结论抑癌基因TCF21可抑制A549细胞的增殖和迁移、诱导凋亡。

Background and objective TCF21, a newly discovered gene, exhibits tumor suppressor function in a variety of tumors. hTis study aims to observe the effects of TCF21 on the proliferation, apoptosis and migration of A549 human lung adenocarcinoma epithelial cells. Methods TCF21 was overexpressed in A549 cells via lentiviral transfection. Fluorescence-based quantitative polymerase chain reaction and Western blot analysis were used to analyze the expression of the target gene. Transwell, proliferation assay, and lfow cytometry were applied to detect the effect of TCF21 overexpression on the migration, proliferation, and apoptosis of A549 cells atfer transfection. Results hTe proliferation and migration of A549 cells were inhib-ited, and the apoptotic rate was increased by overexpressing TCF21. Conclusion hTe tumor suppressor gene, TCF21, signiif-cantly inhibits the proliferation and migration, as well as facilitates early apoptosis of A549 cells.

目的 观察小于扰RNA(siRNA)沉默结直肠癌HT-29细胞TCF21基因对Kiss-1基因表达的影响,以及细胞增殖、侵袭及迁移能力等生物学行为的变化.方法 利用脂质体lipofectamineTM 2000将siRNA稳定转染结直肠癌HT-29细胞,实验分为干扰组、阴性对照组、空白对照组；应用实时定量逆转录聚合酶链反应(RT-qPCR)与Western blot技术检测TCF21与Kiss-1基因mRNA与蛋白的表达水平,分别采用细胞计数试剂盒(CCK-8)法和Transwell小室法检测干扰前后细胞增殖、侵袭与迁移能力的变化.结果 siRNA干扰后成功沉默了HT-29细胞TCF21基因的表达,Kiss-1基因mRNA的表达量为0.58 ±0.02,较对照组的1.00±0.00明显降低(P＜0.05),蛋白表达量为0.3491±0.0009,与对照组比较(0.8485 ±0.0016)亦出现明显下降(P＜0.05),同时细胞增殖、侵袭及迁移能力均明显增强(P＜0.01).结论 沉默TCF21基因的表达可以下调结直肠癌细胞Kiss-1基因的表达水平,并导致相应生物学行为的变化,因此TC F21基因可能是Kiss-1基因的上游调控因子.

Objective To investigate the effect of silencing transcription factor 21 (TCF21) gene expression by small interfering RNA (siRNA) on the expression of Kiss-1 gene and the cell biological behaviors of proliferation,invasion and migration in human colorectal cancer cell line HT-29 in vivo.Methods A specific siRNA molecule fragment for TCF21 was designed,and transfected into HT-29 cells by lipofectamineTM 2000.The experimental group,negative control group and blank control group were set up.Real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression levels of TCF21 and Kiss-1 mRNA at 48 h after transfection.By using Western blotting,the expression levels of TCF21 and Kiss-1 proteins at 72nd h after transfection was examined.The Cell Counting Kit-8 (CCK-8) was used to detect cell proliferation capacity,and Transwell assay to detect the invasion and migration capacity at 72nd h post-transfection.Results The siRNA fragment could silence TCF21 gene ex

目的：寻找在中国人群中具有高敏感性、高特异性的膀胱癌甲基化标志物，以应用于膀胱癌的尿液诊断。方法先用T24细胞系筛选8种文献报道的膀胱癌甲基化标志物，再利用甲基化特异性荧光定量PCR(qMSP)技术分别在28例膀胱癌患者、10例尿路结石伴感染患者和30例健康志愿者尿液样品中检测筛选成功的4种甲基化标志物，计算出不同组合的敏感性和特异性。结果 PCDH17、POU4F2、TCF21、ZNF154的敏感性分别为46．43%、92．86%、39．29%、46．43%，特异性分别为95．00%、97．50%、97．50%、100．00%，最优的诊断标志物为 POU4F2(敏感性92．86%、特异性97．50%)。结论应用qMSP技术，以POU4F2为甲基化标志物在尿液中检测膀胱癌可能会成为一种临床可用的诊断方式。

Objective To identify a panel of novel epigenetic biomarkers with high sensitivity and specificity that can be utilized in detection and diagnosis of bladder cancer using urine sediments. Methods T24 cell lines that had been treated with bisulfite were used to examine the 8 methylated candidates that were previously reported in bladder cancer patients. Methylation levels of the candidate genes were quantified using the urine sediments from 28 bladder cancer patients, 30 healthy volunteers and 10 infected urinary calculi patients by quantitative methylation-specific polymerase chain reaction(qMSP). The four most efficacious and reliable biomarkers were selected after the sensitivity and specificity of each biomarker were further calculated and inspected. Results The sensitivities of PCDH17, POU4F2, TCF21 and ZNF154 in the detection of bladder cancer were 46. 43%, 92. 86%, 39. 29% and 46. 43% respectively;the specificities of these biomarkers were 95. 00%, 97. 50%, 97. 50% and

目的：通过研究米非司酮配伍米索前列醇对早孕蜕膜组织转化生长因子表达的影响，探讨米非司酮配伍米索前列醇终止早孕的作用机制。方法：收集2010年8月-2011年5月在笔者所在医院终止妊娠的健康早孕妇女蜕膜组织41例，分为药流组21例和人流组20例，检测蜕膜组织中转化生长因子的表达水平。结果：药流组转化生长因子表达较低，与人流组比较，差异有统计学意义（P〈O．05）。结论：米非司酮可以降低早孕蝉瞳幺日织TcF—R的嘉扶．诵讨龟癌檄府扶驯终止钎娠的目的．米索前列醇增强或独立发挥了终止妊娠的效果。

Objective:Through the research of mifepristone compatibility misoprostol therapy influenced the expression of transforming growth factor beta in early pregnancies decidual tissue,to discuss the function mechanism of termination of the early pregnancy therapy by mifepristone compatibility misoprostol. Methods:41 cases early pregnancy decidual tissue were collected from August 2010 to May 2011 in our hospital women outpatients,21 cases were divided into drug-abortion group,20 cases were divided into abortion group,then detected the expression of transforming growth factor beta.Results:The expression of drug-abortion group had statistically significant with abortion group(P<0.05),drug-abortion group expression was low,and had large difference of expression level. Conclusion:Mifepristone can reduce the expression of transforming growth factor beta,which can terminate pregnancy through the immune effect,misoprostol can enhance the effect or independent play the effect of termination of preg