登录

双语推荐：p63基因

进入期刊文章
目的：通过构建小干扰RNA （small interfering RNA，siRNA）降低MG-63骨肉瘤细胞环氧合酶-2（COX-2）基因的表达，并进一步研究其对MG-63骨肉瘤细胞增值、侵袭、迁移能力的影响及分子机制。方法设计靶向干扰COX-2基因的siRNA，通过脂质体转染MG-63骨肉瘤细胞，使其抑制MG-63骨肉瘤细胞COX-2基因的表达，后采用噻唑蓝（MTT）比色法、Transwell小室实验研究其对MG-63骨肉瘤细胞增殖、侵袭、迁移能力的影响，采用RFQ-PCR和Western blot分别从基因和蛋白的水平检测MG-63骨肉瘤细胞侵袭性相关因子基质金属酶（MMP-9）的表达及血管内皮生长因子（VEGF）的表达。结果转染MG-63骨肉瘤细胞后，实验组与阴性对照组和空白对照组比较，通过RFQ-PCR和Western blot检测COX-2基因表达降低约90％（P＜0．05），MTT检测MG-63骨肉瘤细胞增值能力明显受到抑制（P＜0．05），Transwell 实验检测 MG-63骨肉瘤细胞侵袭、迁移能力明显下降（P ＜0．05），经 RFQ-PCR、Western blot检测侵袭性相关因子MMP-9和血管内皮生长因子VEGF 的mRNA及蛋白表达降低（P＜0．05）。空白对照组和阴性对照组比较无明显变化，差异无统计学意义（P＜0．05）。结论人MG-63骨肉瘤细胞 COX-2基因被抑制后，MG-63骨
Objective Through establishing the small interfering RNA (siRNA)to decrease the expression of MG-63 osteo-sarcoma cell''s cyclooxygenase-2 (COX-2)gene,and further research the influence and molecular mechanism of the power for proliferation,invasion and migration for MG-63 osteosarcoma cell.Methods Designed the targeted interference COX-2 gene''s siRNA,transfected MG-63 osteosarcoma cell by the lipidosome,so as to suppress the expression of COX-2 gene in MG-63 osteosarcoma cell,then we used the MTT colorimetric method,and Transwell small room experiment to research the influence to the power of proliferation,invasion and migration for MG-63 osteosarcoma cell.Using RFQ-PCR and Western blot to detect the expression of the MG-63 osteosarcoma cell invasive related factor matrix metal enzyme(MMP-9)expres-sion and the expression of vascular endothelial growth factor(VEGF)from the gene level and the protein level.Results Af-ter transfecting the MG-63 osteosarcoma cells,comparing the

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的探讨白花蛇舌草对人骨肉瘤MG-63细胞Bax基因表达的影响。方法采用MTT法检测一定浓度白花蛇舌草注射液对MG-63细胞作用6、12、24、48h后的细胞增殖抑制率,实时荧光PCR(RT-PCR)检测细胞内Bax基因的表达情况。结果白花蛇舌草注射液能明显抑制MG-63细胞增殖,浓度为100μL/mL时,随着作用时间的延长,Bax基因表达明显升高(P0.05)。结论白花蛇舌草注射液可能通过上调Bax基因表达诱导人骨肉瘤MG-63细胞凋亡。
Objective To investigate the effect of hedyotic diffusa willd injection on osteosarcoma MG‐63 cells Bax gene expres‐sion .Methods MTT was be used to detect the inhibition rate of MG‐63 cells by hedyotic diffusa willd injection after 6 ,12 ,24 ,48 h certain concentration ,RT‐PCR was be used to detect the intracellular expression of Bax gene .Results Hedyotic diffusa willd injec‐tion can significantly inhibit the proliferation of MG‐63 cells ,as the concentration was 100 μL/mL ,Bax gene expression was signifi‐cantly increased over time(P<0 .05) .Conclusion Upregulating the expression of Bax gene by hedyotic diffusa willd injection can induced human osteosarcoma M G‐63 cells apoptosis to death .

[ 可能符合您检索需要的词汇 ]

进入期刊文章
通过检测P16基因在宫颈癌中的表达,分析P16基因与宫颈癌预后的关系,评价P16基因在宫颈癌预后预测中的价值。方法:采用免疫组织化学SP法检测P16基因在74例宫颈癌患者组织中的表达,并对P16基因表达与宫颈癌预后的关系进行相关性分析。结果:74例宫颈癌病例中,P16基因的表达阳性率为85%(63/74),P16基因阳性表达与宫颈癌预后呈正相关(P=0.041)。P16基因阳性表达组2年累积生存率为85.2%,P16基因阴性表达组2年累积生存率为100%。P16基因阳性表达组2年累积生存率低于P16基因阴性表达组(P=0.043),两者比较有统计学意义。P16基因阳性表达与临床分期、间质浸润及LVSI呈正相关(P0.05),而与TNM及组织分化无相关性(P0.05)。结论:P16基因的阳性表达与宫颈癌患者的预后密切相关,可作为预测宫颈癌预后的指标之一。
Objective To access the expression of germ P16 in cervical cancer patients , find out the connection between with the expression in germ of P16 and the prognosis of cervical cancer , and discuss whether P16 can role as an indicator to predict the prognosis. Methods The pathological sections of all 74 cases were tested for the presence of P16 germ , using an immunohistochemistry technique. And the results were analyzed to investigate the value of P16 on the prediction of prognosis of cervical cancer. Results Of all 74 cervical cancer cases, there are 63 cases show positive expression of P16, with the positive expression rate of 85% (63/74). The positive expression of gene P16 is associated with the prognosis of cervical cancer (P = 0.041). The cumulative survival rate for two years of the positive expression set is 85.2%, and the negative set 100%, which is statistically significant (P = 0.043). Positiveexpression of P16 is closely related (P 0.05) with TNM and histological differentiat

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的 检测MG-63骨肉瘤细胞Ras相关区域家族1(RASSF1)基因启动子甲基化,并观察5-氮杂胞苷去甲基化对其生物学行为的影响.方法 采用亚硫酸盐测序法(BSP)检测MG-63骨肉瘤细胞RASSF1基因启动子甲基化状态,比较5-氮杂胞苷处理前后RASSF1甲基化状态、基因表达、细胞增殖曲线、细胞周期和凋亡的差异.结果 5-氮杂胞苷处理前RASSF1基因启动子第1～3、第16 CG位点甲基化率为40.0％,第4～15 CG位点甲基化率为60.0％;处理后的MG-63骨肉瘤细胞RASSF1基因启动子第1～16 CG位点甲基化率为0.正常未处理MG-63骨肉瘤细胞RASSF1基因mRNA和蛋白质表达处于低表达状态,5-氮杂胞苷处理后RASSF1基因mRNA和蛋白质表达显著增加,显著高于处理前.5-氮杂胞苷处理后的细胞增殖速度减慢,D1-D8细胞吸光度值显著低于正常未处理的细胞.5-氮杂胞苷处理后的MG-63骨肉瘤细胞Go/G1期和凋亡率显著高于处理前细胞(P＜0.05),S期、G2/M期和增殖指数(PI)显著低于处理前细胞(P＜0.05).结论 MG-63骨肉瘤细胞RASSF1基因启动子处于甲基化和表达抑制状态,5-氮杂胞苷能逆转RASSF1基因启动子甲基化状态,使基因重新表达而抑制细胞增殖并促进其凋亡.
Objective To detect the methylation status of Ras association domain family 1 (RASSF1) in osteosarcoma cells and the effect of 5-azacytidine-induced demethylation on thier biological behaviors.Methods Bisulfite sequencing polymerase chain reaction (BSP) was applied to detect the methylation status of RASSF1 gene promoter in MG-63 Osteosarcoma cell line.The changes of RASSF1 gene promoter methylation status,gene expression,growth curve,cell cycle and apoptosis before and after 5-azacytidine treatment were compared.Results The methylation rate of RASSF1 gene promoter in MG-63 osteosarcoma cell line before 5-azacytidine-induced demethylation was 40.0％ at 1-3 and 16 CG,and 60.0％ at 4-15 CG (60.0％),but that was 0 at 1-16 CG after 5-azacytidine-induced demethylation.The methylation status and gene expression were reversed and resumed.The absorbance after 5-azacytidine-induced demethylation was lower than pre-treatment.The proportion of cells in G0/G1 phase and apoptosis rate aft

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的探讨2型糖尿病（T2DM）遗传易感性与CAPN-10基因多态性的关系。方法采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术对100例T2DM患者（病例组）和100例健康者（对照组）CAPN-10基因SNP19（rs3842570）、SNP43（rs3792267）和SNP63（rs5030952）多态性位点进行基因分型。结果病例组CAPN-10基因43位点的GG基因型频率和G等位基因频率显著高于对照组,差异有统计学意义（P〈0.05）;19位点和63位点的基因型频率、等位基因频率在病例组与对照组分布差异均无统计学意义（P〉0.05）。结论 CAPN-10基因SNP43（rs3842570）位点与T2DM的发生有相关性,而SNP19（rs3842570）和SNP63（rs5030952）位点则与T2DM的发生无相关性。
Objective To observe relationship between the genetic susceptibility and polymorphisms of CAPN-10 gene in type 2 diabetes mellitus (T2DM ) .Methods Restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) method was used to determine the distribution of allele and genotype frequencies of SNP 19 (rs3842570) ,SNP43 (rs3792267) and SNP63 (rs5030952) polymorphism in CAPN-10 gene in 100 T2DM patients (case group) and 100 normal control subjects (control group) .Results The frequencies of GG genotype and G allele of SNP43 in case group was significantly higher than that in control group (P 0 .05) .Conclusion The polymorphism of SNP43 in CAPN-10 gene may contribute to the genetic susceptibility to T2DM ,but SNP19 and SNP63 in CAPN-10 gene may be not related to T 2DM susceptibility possibly .

[ 可能符合您检索需要的词汇 ]

进入期刊文章
背景：造成假体周围骨溶解的完整分子机制目前尚未完全清楚。假体周围骨溶解、吸收是人工关节松动的典型病理生理过程。白细胞介素1通过MAPK信号通路影响骨吸收进程。 目的：实验通过观察siRNA沉默白细胞介素1受体相关激酶4(interleukin-1 receptor-associated kinase-4， IRAK-4)基因表达对人成骨样细胞株MG63的MAPK信号转导通路的影响，为防治人工关节置换后假体周围骨溶解提供实验基础。 方法：以Lipofectamine 2000为载体将IRAK-4-siRNA转染入MG63细胞。实验分为3组，空白组不加入任何转染试剂；对照组以 scrambled siRNA 序列进行转染；沉默组以特异性 IRAK-4-siRNA 序列进行转染。Western blot检测靶细胞细胞外调节蛋白激酶、c-Jun氨基末端激酶和p38MAPK基因的表达。 结果与结论：与对照组相比，靶基因沉默组的IRAK-4 mRNA及蛋白表达水平显著降低(P<0.05)。MG63细胞 IRAK-4表达下调后，与空白组和对照组相比，c-Jun 氨基末端激酶1/2P46、磷酸化细胞外调节蛋白激酶1/2、磷酸化p38MAPK表达下调分别为62%，64%，68%(P <0.05)。结果证实，siRNA沉默IRAK-4基因抑制人成骨样细胞株MG63细胞外调节蛋白激酶、c-Jun氨基末端激酶和p38MAPK基因的表达。
BACKGROUND:The molecular mechanism of periprosthesis osteolysis is not yet completely clear. Periprosthetic osteolysis and absorption is the pathological and physiological process typical of artificial joint loosening. Interleukin-1 can affect bone resorption process through a mitogen-activated protein kinases (MAPK) signaling pathway. OBJECTIVE:To explore the effects of siRNA-induced interleukin-1 receptor-associated kinase-4 gene (IRAK-4) silence on MAPK expression in MG63 cells, which may provide experimental basis for treatment and prevention of periprosthesis osteolysis. METHODS:The siRNA sequences of the target gene, IRAK-4, were constructed and transferred into MG63 cells using Lipofectamine 2000. There were three groups:blank group=MG63 cells, control group=MG63 cells transfected with scrambled IRAK-4siRNA, and silence group=MG63 cells transfected with specific IRAK-4 siRNA. The protein level of extracellular regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen

[ 可能符合您检索需要的词汇 ]

进入期刊文章
本研究采用植物数量性状主基因+多基因混合遗传模型多世代联合分析方法,对耐褐变丝瓜(P1)与易褐变丝瓜(P2)构建的P1、P2、F1、B1、B2和F26个群体果肉褐变特性进行分析。结果显示,普通丝瓜果肉耐褐变遗传符合2对加性-显性-上位性主基因+加性-显性-上位性多基因混合遗传模型(E_0)。其中B1、B2和F2的主基因遗传率分别为50.00%、50.00%和62.27%,多基因遗传率分别为40.07%、42.04%和31.63%,环境方差占总表型方差的6.10%~9.93%。
Six populations (P1, P2, F1, B1, B2 and F2) derived from the cross of anti-browning germplasm(P1) and browning germplasm( P2 ) in luffa were used to study the inheritance of anti-browning by the mixed major-gene plus pol-ygene inheritance model with joint analysis method of multiple generations. The inheritance of anti-browning of luffa fitted two pairs of additive-dominance-epitasis major genes plus additive-dominant-epitasis polygene model ( E 0 ) . The major gene heritabilities of B1 , B2 and F2 were estimated to be 50. 00%, 50. 00% and 62. 27%, respectively, and the polygene heritabilities to be 40. 07%, 42. 04% and 31. 63%, and the environmental variance accounted for 6. 10%-9. 93% of phe-notype variance.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：研究 GRIM-19在人膀胱尿路上皮癌(BTCC)组织中的表达和临床意义，以及与 p63、STAT3表达的相关性，探讨 GRIM-19对 p63、STAT3的影响及其在 BTCC 中的作用及机制。方法采用逆转录聚合酶链反应检测76例 BTCC 组织、45例相应的癌旁组织和30例正常膀胱组织的 GRIM-19 mRNA表达，免疫印迹法检测 p63、STAT3蛋白的表达。结果 GRIM-19 mRNA在76例BTCC组织中的阳性表达率为40.79%，显著低于癌旁组织和正常膀胱组织(分别为66.67%和96.67%)(P均<0.05)；p63、STAT3蛋白在BTCC组织中的表达(分别为82.89%、72.37%)显著高于癌旁组织和正常膀胱组织(P均<0.01)。BTCC组织中GRIM-19 mRNA的低表达与肿瘤的病理分级、淋巴转移密切相关，而与患者性别、年龄、临床分期、肿瘤直径和肿瘤发生情况等无相关性。相关性检验表明 GRIM-19 mRNA与 p63、STAT3表达呈显著负相关(P<0.01)。结论 GRIM-19 mRNA在BTCC组织中的表达下调，提示 GRIM-19作为重要的抑癌基因，可能与凋亡相关基因p63、STAT3在BTCC的发展中起拮抗作用，参与 BTCC 的发生和发展过程，并可能是 BTCC 转移的潜在标志。
Objective To investigate the expression and clinical significance of GRIM-19 and its correlation with p63 and STAT3 in the tissues of human bladder transitional cell carcinoma (BTCC),and to investigate the role of GRIM-19 in the BTCC. Methods Reverse transcription polymerase chain reac-tions and Western blotting techniques were respectively used to detect the expressions of GRIM-1 9 mRNA and the proteins of p63 and STAT3 in 76 cases tissues of BTCC,compared with 45 cases of adjacent noncancerous tissues and 30 cases of normal bladder tissues. Results The expression rate of GRIM-19 mRNA in BTCC tissues was 40.79% ,which was significantly lower than those of adja-cent noncancerous tissues (66.67%)and normal tissues (96.67%)(P 0.05). Expressions of the proteins of p63 and STAT3 were negatively correlated with expression of GRIM-19 (χ2=-17.16 and -9.12,respectively,P<0.01). Conclusions Low expression of GRIM-19 in BTCC might induce the expression of p63 and STAT3 in BTCC.As a tu

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的通过新的维吾尔族妇女宫颈癌组织特异性上调表达基因的分析,探讨宫颈癌发生与基因表达调控的关系及临床意义。方法选择6种新发现的宫颈癌组织特异性上调表达候选基因,设计mRNA特异性引物,对25例宫颈鳞癌(cervical squamous cell carcinoma,CSCC)、22例宫颈内上皮瘤变(cervical intraepithelial neoplasia,CIN)Ⅱ~Ⅲ和25例宫颈炎组织RNA进行半定量RT-PCR鉴定。结果 CSCC与宫颈炎组织比较,SHISA2、SIX1、果蝇同源盒基因(DTL)、桥粒芯糖蛋白-2(DSG2)、NUP62CL和胞嘧啶脱氨酶(APOBE63B)6种基因的mRNA水平均有差异(P0.05);CSCC与CINⅡ~Ⅲ比较,SHISA2、SIX1、APOBEC3B和DSG2 4种基因的mRNA水平有差异(P0.05),而CINⅡ~Ⅲ与宫颈炎比较,只有SHISA2基因的mRNA水平差异有统计学意义(P0.05)。结论 SHISA2、SIX1、DTL、DSG2、NUP62CL和APOBE63B6种基因的转录表达水平变化可能成为维吾尔族妇女宫颈癌发生的早期预警标志物。
Objective To screen novel genes upregulated in cervical cancer,and investigate association of cervical carcinogenesis with the regulation of gene expression and clinical outcome.Methods We selected six newly identified genes specific to,and upregulated in,tissue specimens of cervical carcinoma.After de-signing of mRNA sequence specific primers,the expression level of these genes was detected by semi-quantitative RT-PCR,in a total of 72 cases of fresh tissue specimens from Uyghur women with cervix le-sions including 25 cases of cervical squamous cell carcinoma (CSCC),22 cases of cervical intraepithelial ne-oplasia (CIN)and 25 cases of cervicitis or normal cervix (NC);Results The transcription levels of SHISA2,SIX1,DTL,DSG2,NUP62CL and APOBE63B were significantly different in CSCC from NC (P 0.05).Conclusion The aberrant up-regulation of SHISA2,SIX1,DTL,DSG2,NUP62CL and APOBE63B gene expression may become potential mark-ers for the early diagnosis of cervical carcinoma.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
探讨TAp63 mRNA在多发性骨髓瘤(MM)中的表达及其在MM发病和预后中的可能作用。方法:采用RTPCR检测19例MM患者骨髓及10例非恶性血液病患者骨髓中TAp63 mRNA的表达水平。结果:TAp63 mRNA在MM患者骨髓及非恶性血液病患者骨髓中平均灰度值分别为0.912±0.424、0.315±0.035,两者比较有显著性差异(P0.001);其中ISS分期Ⅲ期患者的TAp63 mRNA平均灰度值显著高于Ⅰ期及Ⅱ期患者(P=0.000),同时MM患者TAp63 mRNA的表达强度与血清β2MG有显著正相关性(r=0.607,P=0.006)。结论:TAp63基因在MM患者中呈现了高表达状态,参与了MM的发生、发展,并与预后相关。
Objective:To investigate the expression of TAp63 mRNA in multiple myeloma ( MM) and its potential role in pathogenesis and prognosis. Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to determine the marrow level of TAp63 mRNA in 19 patients with MM and another 10 of hematologic non-malignancies.Results:TAp63 mRNA expression level was 0.912 ±0.424 for the MM patients and 0.315 ±0.035 for the hematologic non-malignancies.The two groups were significantly different(P<0.001).By the International Staging System(ISS) for multiple myeloma, the mean level of TAp63 mRNA in patients at stage Ⅲ was significantly higher than those at stage Ⅰ and Ⅱ(P=0.000),and the TAp63 mRNA expres-sion was positively related to serum β2MG in MM patients(r=0.607;P=0.607).Conclusion:TAp63 presents a higher expression in patients with MM, suggesting that this gene is involved in the occurrence and development of MM ,and may be an indicator to estimate the prognosis .

[ 可能符合您检索需要的词汇 ]