试验分别采用超临界CO2流体萃取和水蒸汽蒸馏技术提取依兰香花挥发油,超临界CO2流体萃取技术挥发油提取率为3.9%,明显高于水蒸汽蒸馏技术挥发油得率3.0%。采用GC-MS方法对挥发油成分进行分析,两种提取技术所得挥发油组成成分基本一致,确定出依兰香花挥发油成分有β-石竹烯、Germacrene D、苯甲酸苄酯、1,4,7,-Cycloundecatriene,1,5,9,9-tetramethyl-,Z,Z,Z-、以及乙酸薰衣草酯等近20种。试验表明,超临界CO2流体萃取更适用于依兰香花挥发油的提取,并且β-石竹烯可以作为衡量依兰香花挥发油品质的重要参考。
Supercritical CO2 fluid extraction and steam distillation techniques were applied on the volatile oil extrac-tion of Cananga odorata flower, individually,the yield rate of volatile oil from supercritical CO2 fluid extrac-tion is 3.9 %, much higher than that of steam distillation techniques. GC-MS was adopted to analysis the constitutes of the vol-atile oil, the components of volatile oil from the two extraction methods are identical basically, nearly twenty kinds of consti-tutes were identified, including β-caryophyllene, Germacrene D, Benzoic acid benzyl ester, 1,4,7,-Cycloundecatriene,1,5,9,9-tetramethyl-,Z,Z,Z-, and Lavender ester of acetic acid et al.. By comparision, Supercritical CO2 fluid technique is more suit-able to the volatile oil extraction of Cananga odorata flower, and β-caryophyllene can serve as an important reference to mea-sure quality of the volatile oil.