建立了蔬菜中噻虫嗪残留量快速溶剂萃取高效液相色谱的分析方法。使用C18液相色谱柱,以乙腈∶磷酸溶液为流动相,对其进行了定性定量分析,方法的线性相关系数0.99993,加标回收率≥95.24%,相对标准偏差小于3%,该方法步骤简单快速,适用于蔬菜中噻虫嗪农药的快速检测。

We develop a method for the determination of thiamethoxam in vegetable by accelerated sol-vent extraction-HPLC .The method includes the C18 liquid phase chromatography column ,acetonitrile/phosphate (30 :70 ,by volume)(pH≈3 .0) as the mobile phase ,and the UV detector at 263 nm .The linear correlation is 0 .99993 .Recovery rates with standard samples are 95 .24%-99 .91% .Relative standard devi-ation(RSD) is<3% .The method is rapid ,simple ,and readily applicable the rapid determination of thia-methoxam pesticide in vegetable .

丙烯酰胺是一种可能的致癌物,广泛存在于各种食品中,如焙烤类食物、油炸薯条和烤肉等.丙烯酰胺定量检测方法对于准确评估其对人体危害十分必要.综述了食品中丙烯酰胺的检测技术与方法如液质联用技术、液相色谱法、气相色谱法、毛细管电泳法、酶联免疫分析法(ELISA)和生物传感器法等,而酶联免疫分析法(ELISA)和生物传感器法具有快速方便、样品预处理程序简单、可实现批量样品快速检测等优点,将逐渐代替气相色谱和液相色谱法等传统方法,成为食品中丙烯酰胺检测的快速、便捷检测技术方法.

Acrylamide,as probably carcinogen to human beings,exists widely in foods,such as baked foods,deep fried potatoes and barbecue. It is necessary to accurately assess the harm of acrylamide by quantitative determining acrylamide in food. The methods to detect acrylamide in foods,such as LC-MS,liquid chromatography,gas chromatography,capillary electrophoresis,enzyme-linked immunoas-say(ELISA)and by biosensor,were summarized. Detecting by ELISA and biosensor possessed the virtue of rapidness and convenience,simplicity of sample pretreatment and determining samples in batch,which should replace conventional methods,such as gas chromatography or liquid chromatography,become the main quantification methods.

目的高效快速地检测荔枝果皮中根皮苷含量。方法新鲜荔枝果皮阴干,用70%乙醇溶液以超声波浸提法提取,经过D101大孔树脂吸附层析、C18小柱固相萃取分离后,采用高效液相色谱—质谱联用技术(HPLC-MS)检测提取液中根皮苷含量。结果通过与根皮苷标准品对照,高效液相色谱—质谱在提取液中检测到根皮苷。结论采用70%乙醇溶液超声波浸提法可成功从荔枝果皮中提取到根皮苷,用高效液相色谱—质谱可高效快速地检测出其中含量。

Objective To detect the content of phloridzin in litchi peel efficiently and quickly .Methods Fresh litchi peel was air-dried,extracted with 70% ethanol solvent in ultrasonic wave .The extract was then purified with D101 macroporous resin adsorption chromatography ,C18 solid phase extraction .And the component after purifying was identified by high performance liquid chromatograph -mass spectrography ( HPLC-MS) .Results Compared with the HPLC-MS curves of phloridzin standard ,the same curves were detected by HPLC-MS in the litchi peel extract .Conclusions Litchi peel contains phloridzin ,which can be successfully extracted with 70%ethanol solvent diffusion in ultrasonic wave .And the content can be detected by high performance liquid chromatograph -mass spectro-graphy(HPLC-MS) efficiently and quickly.

用分光光度法测定甲醇溶剂中的氨，用气相色谱法测定液氨中的甲醇，快速、简单，准确度高。

The spectrophotometry was used to determine the amount of ammonia in methanol solvent and the gas chromatography was used to determine the amount of methanol in liquid ammonia. This method was rapid, simple and high accuracy.

目的：采用反相高效液相色谱法测定鬼臼毒素软膏的含量及其有关物质。方法：采用ODS C18色谱柱（5μm,4.6mm×200mm）,以甲醇-水（55∶45）为流动相,流速1.0mL·min^-1,检测波长292nm。结果：反相高效液相色谱法测定的线性范围为0.303-0.707mg/mL,相关系数r=0.9998;三种不同浓度供试液精密度分别为1.05%（n=5）、0.87%（n=5）、1.16%（n=5）。结论：采用反相高效液相色谱法测定鬼臼毒素软膏的含量及有关物质,方法简便、快速、准确、专属性好。

Objective:A R-HPLC method was established for the determination of Podophyllotoxin Ointment and its related substances. Methods:The separation was performed on a C18 column (5μm,4.6mm ×200mm). The mobile phase was composed of methanol∶water=55∶45. The flow rate was 1.0mL·min-1. The detection was carried out with a UV detector at 292 nm. Results:The calibration curves were linear between 0.303~0.707mg/mL(r=0.9998). Three tested solution of differ concentration RSD were 1.05%( n=5),0.87%( n=5) and 1.16%( n=5). Conclusion:The method is simple ,rapid,accurate and is suitable for the determination of Podophyllotoxin for the determination of Podophyllotoxin Ointment and its related substances.