本文采用HPLC法测定银蓝通络口服液质量标准,测定其槲皮素,山奈素及异鼠李素三种黄酮醇苷替代原质量标准中只测定槲皮素醇苷含量,从而优化银蓝通络口服液中总黄酮醇苷质量标准。结果表明：槲皮素、山奈素、异鼠李素线性关系良好,r分别为0．9997、0．9999、0．9999；三种组分添加回收率分别为98．25%、94．99%、98．82%,RSD分别为1．03%、0．36%、0．80%；银蓝通络口服液每支含槲皮素醇苷不少于1．10mg的标准。

In order to optimize the quality standard of total flavonoid glycosides in YIN LAN TONG LUO oral liquid, we determined three flavonol glycosides, quercetin、kaempferol and isorhamnetin in the oral liquid by HPLC to replace the old quercetin only method. The results showed that the method had good linear relation-ship,and the correlation coefficient of each component was 0. 999 7、0. 999 9 and 0. 999 8 respectively. Aver-age recoveries of the three components were 98. 25%、94. 99%、98. 82%,RSD of each group were 1. 03%、0. 36%、0. 80%. Quercetin content standard of no less than 1. 10 mg/bottle was reached. This method can be applied for monitoring flavonol glycosides quality of YIN LAN TONG LUO oral liquid.

对茶条槭(Acer ginnala Maxim.)叶的乙酸乙酯部位化学成分进行了系统研究。采用硅胶柱色谱法、半制备高效液相色谱法以及Sephadex LH-20凝胶柱色谱法等方法进行分离纯化,通过质谱、核磁共振谱等光谱数据鉴定化合物结构。结果分离得到14个化合物,分别鉴定为槲皮素-3-O-α-L-(3″-没食子酰基)-鼠李糖苷(1)、5,7,2′,3′,4′-五羟基黄酮(2)、山柰酚(3)、山柰酚-3-O-β-D-吡喃半乳糖苷(4)、槲皮素-3-O-β-D-来苏糖苷(5)、槲皮素(6)、槲皮素-3-O-β-D-吡喃半乳糖苷(7)、槲皮素-3-O-(2″-没食子酰基)-α-L-阿拉伯吡喃糖苷(8)、槲皮素-3-O-a-(2″-没食子酰基)-鼠李糖苷(9)、山柰酚-3-O-a-(2″-没食子酰基)-鼠李糖苷(10)、槭单宁(11)、没食子酸(12)、没食子酸甲酯(13)、β-谷甾醇(14)。其中化合物1,2,5,9,10为本属中首次分离得到,化合物4和8为茶条槭中首次分离得到。

To investigate the chemical constituents from ethyl acetate extracts of the leaves of Acer gin-nala Maxim,solvent extraction,Silica gel and Sephadex LH-20 column chromatography were used for isolation and their structures were identified by means of spectroscopic and chemical data.Fourteen com-pounds were isolated and identified as quercetin-3-O-α-L-(3″-galloyl)-rhamnoside(1),5,7,2′,3′,4′-pentahydroxy flavone(2),kaempferol(3),kaempferol-3-O-β-D-galactopyranoside(4),quercetin-3-O-β-D-lyxoside (5),quercetin (6),quercetin-3-O-β-D-galactopyranoside (7),quercetin-3-O-α-L-(2″-gal-loyl)-arabinopyranosid (8),quercetin-3-O-α-L-(2″-galloyl)-rhamnoside (9),kaempferol-3-O-α-L-(2″-galloyl)-rhamnoside(10),gallic acid(11),methyl gallate(12),acertannin(13)andβ-sitosterol(14). Compounds 4 and 8 are isolated from the plants in genus Acer for the first time and compounds 1,2,5, 9 and 10 are isolated from the plants Acer ginnala Maxim.for the first time.

目的 采用反相高效液相色谱法(HPLC)分析萹蓄中杨梅苷和槲皮苷的含量.方法 以Agilent LC-C18 (250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1％磷酸溶液(18∶82)作为流动相,流速为1.0 ml/min,柱温:30℃；检测波长:256 nm.结果 杨梅苷和槲皮苷分别在1.22～24.30 tg/ml和0.77～15.40 μg/ml范围内有良好的线性关系,平均加样回收率分别为98.9％、99.6％,RSD分别为1.11％、1.09％.结论 本方法可用于萹蓄中杨梅苷和槲皮苷的定量分析.

Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.Methods The Agilent LC-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(18 ∶ 82),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The calibration curve was linear within a range of 1.22～24.30 μg/ml and 0.77～15.40 μg/ml,the average recovery of this method was 98.9％,99.6％ and the RSD was 1.11％,1.09％,for the myricetrin and quercitroside respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..

右旋糖酐蔗糖酶是一种以蔗糖为唯一底物，将蔗糖分子中D-葡萄糖基催化转移到受体分子上的葡萄糖基转移酶。利用右旋糖酐蔗糖酶的转糖基作用，以蔗糖为葡萄糖糖基供体，槲皮素为糖基受体，对槲皮素糖苷的酶法合成进行了探索。通过对该酶催化反应体系、催化反应条件及产物分析的研究，结果表明：在25℃下，右旋糖酐蔗糖酶能够在30％DMSO-70％乙酸-乙酸钙（0.02 mol/L，pH值5.4）的反应体系中催化合成一种槲皮素葡萄糖苷，在这个反应体系下，以10％的蔗糖作为糖基供体，槲皮素为糖基受体，右旋糖酐蔗糖酶活力为40 U/mL，转速为150 r/min，槲皮素糖苷的转化率最高，可达39.5％。通过质谱分析确定是一种槲皮素单糖苷，分子量为464。该研究结果为黄酮类物质的糖基化修饰奠定了基础。

Dextransucrase is a glucosyltransferases catalyzed by transferring D-glucopyranosyl units in sucrose to the receptor molecule to synthesize a-glucan or oligosaccharides.This paper explored the synthesis of quercetin glycoside by using sucrose as glucose glycosyl donor, quercetin as glycosyl receptor and applying the transglycosylation of dextransucrase.Enzymatic glucosylation in aqueous-organic solvents, the conditions of catalytic reaction and the structure of the product were also studied.The result showed that a new quercetin glycoside was achieved after reaction catalyzed by dextransucrase in a mixture of DMSO ( 30%) /Acetic acid calcium acetate buffer (70%)(0.02 mol/L,pH=5.4)at the 25℃.Under this reaction system, using 10%of sucrose as glucose glycosyl donor, quercetin as glucose glycosyl receptor and 40 U/mL dextransucrase, the stirring speed at 150 r/min, the conversion of quercetin glycoside could be high, up to 39.5%.The product was characterized by MS and confirmed

运用多种色谱法进行分离纯化,并通过理化性质和波谱数据对化合物进行结构鉴定,研究葫芦茶地上部分的化学成分。结果表明：从葫芦茶地上部分分离并鉴定了13个化合物,分别为山柰酚（1）、山柰酚-3-O-α-L-鼠李糖苷（2）、山柰酚-3-O-β-D-葡萄糖苷（3）、山柰酚-3-O-β-D-芸香糖苷（4）、槲皮素-3-O-α-L-鼠李糖苷（5）、山柰酚-3-O-α-L-鼠李糖（1→6）-β-D-半乳糖苷（6）、槲皮素-3-O-β-D-葡萄糖苷（7）、槲皮素-3-O-α-L-鼠李糖（1→6）-β-D-半乳糖苷（8）、芦丁（9）、间苯三酚-O-β-D-葡萄糖苷（10）、对羟基桂皮酸（11）、RoseosideⅡ（12）、儿茶素（13）。其中化合物2、4、5、6、8、9、10、12为首次从该植物中分离得到。

Compounds were separated by various chromatographic techniques to study the chemical constituents of the air part of Tadehagi trquetrum .Their structures were identified by analyzing their physicochemical properties and spectral data.The results showed that thirteen compounds were purified and determined as kaempferol(1),kaempferol-3-O -α-L-rhamnoside(2),kaempferol-3-O -β-D-glucoside(3),kaempferol-3-O -β-D-rutinoside(4),quercetin-3-O -α- L-rhamnoside(5),kaempferol-3-O -α-L-rhamnoside(1 →6)-β-D-galactopyranoside(6),querceitin-3-O -β-D-glucopyranosi(7),quercetin-3-O -α-L-rhamnoside(1→6)-β-D-galactopyranoside(8),rutin(9),phloroglucinol-1-O -β-D-glucopyranoside(10),naringenic acid(11),roseosideⅡ(12),(+)-catechin(13).Compounds 2,4,5,6,8,9,10,12 were isolated from Tadehagi trquetrum for the first time.