In this paper,the burst of high-temperature superheater tubes of the boiler was studied. Several methods were adopted such as macroscopic analysis, endoscopic, thickness measuring, micro hardness testing, mechanical property testing, spectral analysis, energy spectrum analysis, metallographic analysis etc. The results showed that the microstructure didn''t comply with the standard, which made the micro cracks. It developed further and leaded to momentary overheating squib of the burst of high-temperature superheater tubes.