目的检测脑卒中后抑郁患者血清脑源性神经营养因子并分析其影响因素。方法选择脑卒中后抑郁患者和健康者作为研究对象,并分析2组研究对象血清脑源性神经营养因子的差异,比较脑卒中后抑郁患者脑源性神经营养因子在不同临床生化指标中的差异及其相关性。结果脑卒中后抑郁患者血清脑源性神经营养因子低于健康对照者,差异有统计学意义（P〈0.05）。脑源性神经营养因子在性别、年龄、卒中病因、病灶部位、病灶分布和卒中部位中的差异无统计学意义（P〉0.05）,在吸烟史、酗酒史、家族史、抑郁评分和美国国立卫生研究院卒中量表（NIHSS）评分中的差异有统计学意义（P〈0.05）。脑源性神经营养因子与吸烟史、酗酒史、家族史、抑郁评分和NIHSS评分均呈负相关（均rs〈0,P〈0.05）。结论脑卒中后抑郁患者血清脑源性神经营养因子明显低于健康者,其水平与吸烟史、酗酒史、家族史、抑郁评分和NIHSS评分均呈负相关。

Objective To detect the serum levels of brain-derived neurotropic factor (BDNF) in cases with post-stroke depression (PSD) and analyze its influencing factors .Methods The cases with PSD and healthy people were chosen to detect the serum BDNF ,and the differences were compared .The relationship between clinic pathologi-cal factors and BDNF in PSD cases was analyzed .Results The serum BDNF in PSD cases was significantly lower than in healthy people .The differences of BDNF in PSD cases showed no statistics significances in agenda ,age ,cause of disease ,location ,distribution and position ,while they were statistics significant in smoking history ,history of alco-hol abuse ,family history ,depression score and NIHSS score .The BDNF in PSD cases correlated negatively with smoking history ,history of alcohol abuse ,family history ,depression score and NIHSS score .Conclusion The serum BDNF in PSD cases is significantly lower than in healthy people ,its levels correlates negatively with sm

背景：研究显示，脑源性神经营养因子可在骨与牙组织中表达，其在骨与牙组织发育代谢中的作用及进一步应用逐渐成为研究热点。目的：综述并分析脑源性神经营养因子在骨与牙组织发育代谢中的作用及其机制。方法：于2014年8月以“脑源性神经营养因子、酪氨酸激酶B、p75神经营养因子受体、信号通路、骨、牙、成骨细胞、破骨细胞”为中文关键词，以“Brain-derived neurotrophic factor，TrkB，p75NTR结果与结论：脑源性神经营养因子在体内许多组织细胞中均有表达。通过结合不同受体调控细胞存活与程序性死亡。脑源性神经营养因子在骨与牙组织中可作用于靶细胞，促进或抑制细胞增殖、分化，与骨与牙组织关系密切，在骨与牙的发育、修复重建各方面发挥作用。其作用机制主要是通过与TrkB受体结合，激活下游通路，影响间充质干细胞、成骨细胞、软骨细胞、牙周膜细胞等细胞分化及增殖，p75，signaling，bone， tooth，osteoblasts，osteoclasts”为英文关键词，采用计算机检万方医学网和 PubMed数据库，筛选有关脑源性神经营养因子作用于骨与牙组织的文章53篇进行分析。NTR受体与TrkB受体两者间的相互作用可能是影响细胞向分化或增殖发展的因素之一。

BACKGROUND:Brain-derived neurotrophic factor has been detected in bone and tooth, and its role in development and metabolism of bone and tooth tissue as wel as its clinical application has become a hot spot. OBJECTIVE: To summarize and analyze the effect and mechanism of brain-derived neurotrophic factor in development and metabolism of bone and tooth tissues. METHODS: Papers addressing the effect of brain-derived neurotrophic factor in bone and tooth tissue were retrieved by computer in Wanfang and PubMed databases with the key words of “brain-derived neurotrophic factor, TrkB, p75NTR, signaling, bone, tooth, osteoblasts, osteoclasts” in Chinese and English, respectively. A total of 53 papers were included for review. RESULTS AND CONCLUSION:Brain-derived neurotrophic factor can be detected in various tissuesin vivo, and can regulate cel survival and apoptosis through binding its two receptors. Brain-derived neurotrophic factor in bone and tooth tissue can bind to target ce

目的探讨脑源性神经营养因子Val66Met多态性与脑出血的关系。方法采用限制性内切酶片段长度多态性技术检测脑源性神经营养因子Val66Met多态性,在110例脑出血患者和101名健康对照组中的分布;比较病例组、对照组基因型、等位基因分布差异。结果 (1)脑出血组和对照组A基因型分布频率分别为0.40和0.20,显著高于对照组,差异具有统计学意义(x2=13.81,P=0.001);(2)两组等位基因分布频率差异也具有统计学意义,差异具有统计学意义(x2=13.12,P=0.000)。结论脑源性神经营养因子Val66Met多态性的A等位基因同脑出血相关,可能该病的易感基因。

Objective To explore the possible association between Val66Met polymorphism of BDNF and cerebral hemorrhage in Chinese Han population. Methods We analyzed polymorphisms of the BDNF Val66Met polymorphism with a lfragmentation length polymorphism(RFLP)-based method in 110 patients with cerebral hemorrhage and 101 unrelated healthy control subjects. Results (1)The AA genotypes frequencies of Val66Met polymorphism in cerebral hemorrhage patients and control subjects were 0.40 and 0.20,respectively,the difference was statistic(x2=13.81,P=0.001) (2)The allele frequencies of Val66Met polymorphism in cerebral hemorrhage and control group were significant(x2=13.12,P=0.000).And the allele A frequency of Val66Met in cerebral hemorrhage patients was significantly higher than that of control subjects (P < 0.01). Conclusion This study suggests that the BDNF Val66Met may be the major risk factors in increasing susceptibility to cerebral hemorrhage in Chinese Han population.

背景：选择合适的诱导方法诱导人胎盘间充质干细胞分化为神经元样细胞是临床治疗神经损伤的关键。 目的：在人胎盘间充质干细胞中加入商品化的人胚胎脑组织蛋白提取物，观察其诱导分化为神经元样细胞的可行性。 方法：采用酶消化法分离培养人胎盘间充质干细胞，传至第3代，将人早期胚胎脑组织蛋白提取物加入诱导培养基培养6 d，加入浓度为20 nmol/L全反式维甲酸和500μg/L音猬因子培养3 d，换新的培养液，包含体积分数为10%胎牛血清，10μg/L脑源性神经营养因子，20μg/L神经胶质细胞源性神经营养因子，50μg/L胰岛素样生长因子Ⅱ型继续培养3 d。 结果与结论：胎盘组织经酶消化后获得贴壁细胞，传至第2代细胞形态为梭形，成漩涡样生长，传至第3代细胞形态较均一。诱导后细胞经免疫细胞化学法检测均表达神经元特异性烯醇化酶、巢蛋白、胶质纤维酸性蛋白、神经丝蛋白、神经生长相关蛋白43；ELISA法检测细胞培养上清脑源性神经营养因子、神经营养因子3、神经营养因子4为阳性表达；RT-PCR检测细胞微管相关蛋白、神经元特异性烯醇化酶、神经丝蛋白、神经生长相关蛋白43均为阳性表达。结果表明人胚胎脑组织蛋白提取物使人胎盘间充质干细胞诱导分化为神经元样细胞。

BACKGROUND:It is a key to choose an appropriate method to trans-differentiate mesenchymal stem cels from human placenta into neuron-like cels for clinical treatment of neural system injury. OBJECTIVE: To observe the feasibility of the neuronal differentiation of mesenchymal stem cels from human placenta with protein extracts of human embryonic brain tissue. METHODS:Mesenchymal stem cels from human placenta were isolated and cultured using enzyme digestion method. The third passage of cels were incubated in induction medium containing protein extracts of human embryonic brain tissue for 6 days, and then cultured in culture medium containing 20 nmol/L al-trans retinoic acid and 500 μg/L sonic hedgehog for 3 days, folowed by 3-day continuous culture in 10 μg/L brain-derived neurotrophic factor, 20 μg/L glial cel line-derived neurotrophic factor, 50 μg/L insulin-like growth factor type II RESULTS AND CONCLUSION:The adherent cels were obtained after enzyme digestion of placent

脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)是中枢神经系统中最普遍的神经营养因子之一.在神经系统的发育和成熟过程中,BDNF在维持神经元功能、促进神经元损伤后再生修复以及防止神经元变性等方面均发挥着重要作用.目前,很多学者正致力于BDNF治疗脑缺血的研究,并已取得了一些进展.文章对BDNF的分子生物学特征、生物学功能、在脑缺血中的作用和机制以及作为脑缺血干预靶点的可能性进行了综述.

Brain-derived neurotrophic factor (BDNF) is one of the most prevalent growth factors in the central nervous system (CNS).In the development and maturation processes of the nervous system,BDNF plays an important role in maintaining neuronal function,promoting neuronal regeneration after injury,and preventing neuronal degeneration,etc.At present,many researchers are being dedicated to the research of BDNF for treatment of brain ischemia and have achieved some progress.This article reviews the molecular biological characteristics and biological function of BDNF,roles and mechanisms in cerebral ischemia,and the possibility as an intervention target of cerebral ischemia.