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双语推荐：艾叶

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采用水蒸气蒸馏法提取了艾叶挥发油,用气相色谱-质谱法(GC/MS)对其化学成分进行了分析,比较了6种采集期不同的艾叶挥发油的含量和化学成分.结果表明:6种采集期艾叶中挥发油质量分数分别为0.607%,0.750%,0.953%,0.884%,0.751%,0.680%,鉴定出的化学成分数目依次为29,32,29,27,34和28种,有17种相同的化合物被检出.通过比较艾叶挥发油主要成分,如1,8-桉叶油素、樟脑、龙脑、4-萜烯醇等时发现,6月2日采集的艾叶中的挥发油含量最高,品质最好,6月上旬为艾叶的最佳采收期.
To compare and analyze the yield and chemical components of volatile oil from Artermisia argyi Levl. et Vant. gathered in six different growing periods, the volatile oil was extracted by steam distillation and the chemical components was analyzed by GC/MS. The results showed that the mass fraction of the oil yield from 6 different growing periods was 0. 607%, 0. 750%, 0. 953%, 0. 884, 0. 751%, 0. 680% respectively and 29,32,29,27,34 and 28 components were separated and identified, among which there were 17 are common to all the extractions. An assay of the main components from the volatile oil( eucalyptol, camphor, borneol, terpinen-4-ol, etc. ) revealed that the highest yield and the best quality was obtained from those gathered at June 2. Early June might be the best harvest time for Artemisiae argyi Levl. et Vant.

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为从6种不同采集期的艾叶中提取正构烷烃,并比较其成分和含量,寻找艾叶的最佳采收期,采用梯度溶剂萃取法提取了艾叶正构烷烃,用气相色谱-质谱法分析了其化学成分.结果表明:6种不同采集期艾叶的正构烷烃成分中含量最高的是C31,不同正构烷烃含量呈明显的奇偶优势分布,3号和4号长链正构烷烃含量较高.故6月上旬为艾叶的最佳采收期.
To compare and analyze the chemical constituents and the yield of n-alkanes extracted from Artemisia argyi gathered from six different growing periods , and find the best harvest time for Artemisia argyi.The n-alkanes were extracted by gradient solvent and the chemical constituents were analyzed by GC-MS.The results indicated that the component with highest percentage is C 31 , and the n-alkane content showed an odd-even predominance distribution .Sample 3# and 4#offered higher yields of long-chain alkanes , corresponding to the best harvest season of early June .

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以艾叶油为原料,复合表面活性剂为乳化剂,利用相转化法制备了艾叶油乳液。研究了乳化工艺对乳液粒径及其分布的影响,采用扫描电镜对乳液形貌进行了表征,并测定了乳液整理锦纶袜子的驱蚊效果。结果显示,艾叶油驱蚊乳液最佳制备工艺为：乳化剂HLB值8,乳化剂质量分数3%,艾叶油质量分数18%,剪切时间8 min；电镜观察显示,艾叶油乳液粒径较小且分布均匀；整理锦纶丝袜具有良好的驱蚊效果。
The artemisia oil emulsion was prepared by the phase transformation method with artemisia oil as raw material and compound surfactant as emulsifier. The effects of emulsification process on particle size and its distribution of emulsion were investigated. The morphology of artemisia oil emulsion was characterized by SEM, and the effect of the nylon socks finished with artemisia oil emulsion was measured. The results showed that the optimal emulsion process was as fol ows: HLB value of compound emulsifier was 8, mass fraction of emulsifier was 3%, mass fraction of artemisia oil was 18%, and shearing time was 8 min. The SEM photos showed that particle size of artemisia oil emulsion was smal and its distribution was uniformity. The nylon socks finished by artemisia oil emulsion had good mosquito repel ent effect.

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目的：观察苍术艾叶香空气消毒的效果。方法：在制剂室使用苍术艾叶香熏蒸空气消毒，监测消毒前后细菌菌落数，并与紫外线照射空气消毒及甲醛蒸汽消毒效果进行比较。结果：苍术艾叶香熏蒸空气消毒法与紫外线照射空气消毒及甲醛蒸汽消毒效果比较，有明显差异（P＜0.05）。结论：苍术艾叶香空气消毒法能有效杀灭空气中的细菌，且操作方法简单，对人体无不良作用，值得推广。
Objective To study the effects of air disinfection of burning Rhizoma atractylodis and Artemisia argyi. Methods Disinfect the air in pharmaceutical preparation by burning Rhizoma atractylodis and Artemisia argyi, Monitoring the number of bacterial colonies before and after disinfection and compared the effects of air disinfection by Ultraviolet ray and formaldehyde steam. Results The effect of burning Rhizoma atractylodis and Artemisia argyi is superior to Ultraviolet ray and formaldehyde steam. Conclusion The bacteria can be effectively sterilized by burning Rhizoma atractylodis and Artemisia argyi, it can be done easily and do no harm to man, so it worth promoting.

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测定了野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内几种酶活力的影响。结果表明:野艾蒿精油对双斑萤叶甲成虫具有明显的熏蒸作用,野艾蒿精油剂量为10μL/L,处理8 h后校正死亡率为100%,在12 h后各精油处理组的校正死亡率均80%。经熏蒸处理24 h后,野艾蒿精油对双斑萤叶甲成虫体内的乙酰胆碱酯酶(AChE)活力抑制作用明显;对双斑萤叶甲成虫体内的α-乙酸萘酯酶(α-NACarE)活力、酸性磷酸酯酶(ACP)活力、碱性磷酸酯酶(ALP)活力均表现出抑制作用。各处理组的谷胱甘肽-S-转移酶(GSTs)活力均高于对照组,野艾蒿精油对双斑萤叶甲成虫体内的谷胱甘肽-S-转移酶(GSTs)活力表现出明显的诱导作用。野艾蒿精油对双斑萤叶甲成虫体内过氧化物酶(POD)活力的影响不明显。
We test the effects of essential oil from Artemigate lavandulaefolia DC .on fumigation activity and several enzymes of Monolepta hieroglyphica (Motschulsky)adults .The result showed that the essen-tial oil from Artemigate lavandulaefolia DC .had obvious fumigation against Monolepta hieroglyphica (Motschulsky)adults,and the corrected mortality was 1 00%when the dose of essential oil from Artemi-gate lavandulaefolia DC .was 1 0μL/L and 8 h after treatment,and the corrected mortality of all the treatment groups reached 80%or more after 1 2 h .Essential oil from Artemigate lavandulaefolia DC .sig-nificantly inhibited AChE activity;the activities ofα-NACarE and ACP and ALP demonstrated inhibition in the body of Monolepta hieroglyphica (Motschulsky)adults .GSTs activity of all treatment groups was higher than that of the control group,and GSTs activity showed obvious induction .POD activity was not obviously affected .

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目的：观察艾叶油对肺心病模型大鼠肺动脉高压的影响。方法：40只SD大鼠随机分为正常对照组、模型组和艾叶油高、低剂量组,每组10只。采用野百合碱（60mg/kg）腹腔注射建立大鼠肺心病模型,造模后正常对照组、模型组每天给予生理盐水灌胃,艾叶油高、低剂量组给于艾叶油混悬液（1.8、0.2g/kg）灌胃,连续21天。采用右心导管法测定平均右心室压（mRVP）、平均肺动脉压（mPAP）和最大肺动脉压力（Max mPAP）,处死动物测定称取肺湿重（wW）、右心室游离壁（RV）、左心室加心室间隔（LV＋S）质量,计算肺指数（LI）和右心肥厚指数（RVHI）。结果：模型组大鼠mRVP、mPAP和Max mPAP显著高于正常对照组（P〈0.05或P〈0.01）,艾叶油低、高剂量组大鼠mPAP、mRVP和Max mRVP均显著低于模型组（P〈0.05或P〈0.01）;模型组大鼠RV、LV＋S、wW、RVHI及LI均显著大于正常对照组（P均〈0.01）;艾叶油高剂量组大鼠wW、RVHI显著低于模型组（P〈0.05）。结论：艾叶油对野百合碱诱导的大鼠肺心病肺动脉高压有一定的干预作用。
Objective:To observe the effect of Chinese mugwort leaf oil on pulmonary arterial hypertension in rats with pulmonary heart disease. Methods:Forty SD rats were randomly divided into control,model,and low-dose and high-dose drug groups,10 rats in each group. The animal model was established by intraperitoneally injecting monocrotaline of 60 mg/kg in the model and drug groups. Rats in the control and model groups were given saline by gavage for 21 days. The low- and high-dose groups were administered with Chinese mugwort leaf oil suspension in the same way in the same period as the above described. The mean right ventricular pressure(mRVP),mean pulmonary arterial pressure (mPAP),and max mPAP were measured by using right cardiac catheterization. The rats were then sacrificed for determination of lung wet weight (wW) and the weight of right ventricule (RV) and left ventricule plus septum (LV+S). Lung index and right ventricular hypertrophy index were calculated. Results:The mRVP,mPAP,max m

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目的应用快速液相色谱-高分辨飞行时间质谱（RRLC-TOFMS）技术对中药艾叶中化学成分进行快速鉴别。方法色谱分离采用Agilent Eclipse C18色谱柱（2.1 mm×100 mm,1.8μm）;流动相：乙腈（A）-0.1%甲酸水溶液（B）,梯度洗脱,A相随时间的变化：5%~30%（0~12 min）,30%~80%（12~25 min）,80%（25~30 min）,进样量1μl,流速0.35 ml/min,柱温40℃。TOFMS、电喷雾离子源（ESI）、正离子模式、质量数扫描范围m/z 100~1 500。结果一次性鉴别出艾叶中31种化学成分。结论建立基于RRLC-TOFMS技术对艾叶中化学成分快速鉴别的方法,为中药艾叶的质量控制及体内的深入研究奠定基础。
Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatog-raphy-time of flight mass spectrometry (RRLC-TOFMS).Methods The separation was performed on an Agilent Eclipse C18 column (2.1 mm ×100 mm,1.8 μm ).The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B) were in gradient elu-tion.The flowing rate was 0.35 ml/min, the injection volume was 1μl and the temperature of column was 40℃.Time of flight mass spectrometer ( TOFMS) with electro spray ion source ( ESI) was applied to qualitative analysis under the positive ion mode, and mass scan range was m/z 100-1 500 .Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally .Conclu-sion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been suc-cessfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.

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目的：通过正交试验法优化艾叶挥发油提取工艺。方法采用正交试验法考察工艺，其中考察因素为提取时间（A）、料液比（B）和浸泡时间（C），每个因素取3个水平，以得油率为考察指标对艾叶挥发油的提取工艺进行优选。结果艾叶挥发油最佳提取条件为料液比1：10、浸泡4 h、提取3 h，平均得油率为0.416%，RSD=2.64%（n=3）。结论优选的提取工艺得油率较高，工艺稳定可行，可为实际生产提供依据。
Objective To optimize the best extracting process of essential oil of Folium A rtemisiae argyi. Methods The orthogonal test was applied. The following factors with three levels were considered:extracting time (A), the proportion of material to solvent (B), and soaking time (C). Extracting yield was investigated as the evaluation indicators. Results The best extracting process was obtained at the following conditions:ratio of solid to liquid of 1:10, soaking time of 4 h, extraction time of 3 h. The average oil production rate was 0.416%, RSD=2.64%(n = 3). Conclusion The preferred process has higher oil yield, it is stable, feasible, and can provide basis for actual production.

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利用响应面分析法(Response Surface Methodology,RSM)对艾叶多糖提取工艺进行优化。方法:在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用三因素三水平的响应面分析法,以获得多元二次线性回归方程,以多糖提取率为响应值作响应面和等高线。结果:确定了水浸提艾叶多糖最佳工艺条件:浸提温度99℃,浸提时间2.3h,水料比20。结论:在此条件下,艾叶多糖的提取率可达3.017%,与理论预测值3.096%的相对误差为2.6%。
AIM: To optimize the extraction of folium Artemisia argyi polysaccharides by the use of response surface methodoloy.METHODS : Experiment factors and levels were first selected on the ground of one factor test.Along with the central composite experimental design principles,the response surface methodoloy with three factors and three levels was adopted in search of multiple quadratic linear regression.Response surface and contour were finally chosen as the extraction rate and the response value respectively.RESULTS: The optimum extraction conditions of the polysaccharides from folium Artemisia argyi were concluded as follows : extraction temperature was at 99 ℃,extraction time was 2.3 hours,ratio of 20.CONCLUSION : Under these conditions,the yield of folium Artemisia argyi polysaecarides is up to 3.017%,extraction rate of the predictive best polysaccharides is 3.096%,and the relative error is 2.6%.

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目的：通过体外实验观察艾叶乙酸乙酯提取物对乙型肝炎病毒（简称HBV）的抑制作用。方法：取HepG2.2.15细胞,以1.6、8.0、40.0、200.0和500.0 mg/L的艾叶乙酸乙酯提取物处理72 h后,采用MTT法测定细胞生长抑制率,计算半数毒性浓度。另取HepG2.2.15细胞,分别以0、10、20和40 mg/L艾叶乙酸乙酯提取物及100 mg/L拉米夫定（阳性对照）处理3、6、9 d,采用酶联免疫法检测细胞上清液中乙肝表面抗原、e抗原,用荧光定量PCR测定HBV DNA拷贝数,分别计算抑制率和半数抑制浓度,最后计算治疗指数。结果：艾叶乙酸乙酯提取物对HepG2.2.15细胞的半数毒性浓度为104.80 mg/L,对乙肝表面抗原、e抗原及HBV DNA的半数抑制浓度分别是1.26、8.06和38.97 mg/L,并呈剂量依赖性。治疗指数分别为HBsAg 83.2、e抗原13.0和HBV DNA 2.7。结论：艾叶乙酸乙酯提取物在体外对HBV有明显抑制作用。
Methods:HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 1.6,8.0,40.0,200.0 and 500 .0 mg/L for 72 h; the inhibition rate of cell growth was determined by MTT and TC50 was calculated .Another HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 0,10,20 and 40 mg/L and lamivudine (positive control) at 100 mg/L for 3,6,and 9 days; ELISA was used to determine the contents of HBsAg and HBeAg in cell supernatant , and FQ-PCR was used to determine the HBV DNA copies and the inhibition rate and IC50 were calculat-ed, and then the therapeutic index was calculated accordingly .Results: The TC50 of ethyl acetate extracts was 104 .80 mg/L, and the IC50 for HBsAg, HBeAg and HBV DNA was 1.26,8.06,and 38.97 mg/L,and the therapeutic index for the above 3 indexes was 83.2, 13.0, and 2.7.Conclusion:The extracts from folium artemisiae argyi have obvious inhibitory effects on hepatitis B virus in vitro .

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