目的：建立同时测定补骨脂中补骨脂素、异补骨脂素和补骨脂酚3种主要有效成分含量的高效液相色谱方法，比较补骨脂盐炙品炮制前后三种成分含量的变化。方法：采用盐炙法对7个不同地区来源的补骨脂进行炮制，利用高效液相色谱法，甲醇-水梯度洗脱，检测波长246nm，测定炮制前后补骨脂素、异补骨脂素及补骨脂酚的含量。结果：盐炙法使补骨脂中的补骨脂素和异补骨脂素含量增加，使补骨脂酚的含量减少。结论：盐炙法使补骨脂中的三种主要有效成分含量发生了变化。

Objective:To establish a quantitative method of determination of psoralen,isopsoralen and bakuchiol in fruits of Psoralea carylifolia. and salt-processed one,and compare changes of the three kinds of component between them. Method:The content of psoralen,isopsoralen and bakuchiol in the salt-processed one from 7 different areas were determined by HPLC-gradient elution, determined on 246nm wavelength. Results:The content of psoralen and isopsoralen in salt-processed one were raising,and the content of bakuchiol was reducing. Conclusions:The method of salt process could change the content of psoralen,isopsoralen and bakuchiol in fruits of Psoralea carylifolia.

目的为明确补骨脂的功效和毒性物质基础、功效和毒性的相关性以及为今后安全标准制订和进行功效物质基础下补骨脂的毒性研究提供文献依据和研究思路。方法对文献古籍中对补骨脂毒性的记载及近十几年来补骨脂国内外相关文献报道进行整理、分析与归纳。结果历代古籍对补骨脂毒性记载甚少,现代研究仅局限于补骨脂药理、毒理、化学成分等独立性研究,缺少其功效、毒性、物质基础三者关联性研究。因此,应依托功效和物质基础,制订完善的补骨脂安全使用标准,使其毒性可被科学控制。结论只有在补骨脂功效表达和功效物质基础分离与控制的过程中,进行毒性的研究和毒性物质基础的安全控制,才能提出切合补骨脂临床使用过程中药品不良反应的预警方案和早期诊疗措施,确保补骨脂临床用药安全。

ObjectiveToprovideliteratureevidenceandresearchideasfordeterminingthecorrelationofefficacious and toxic substance basis as well as the correlation of its efficacy and toxicity of Psoralea corylifolia, making safety standards, and studying its toxicity based on its efficacious substance basis. Methods References in old days and last decades at home and abroad about Psoralea corylifolia were collated, analyzed and summarized. Results The references in old days were lacking in the records of toxicity of Psoralea corylifolia. The recent researches were confined to the isolated aspect such as pharmacology, toxicology, chemical constituents and lacked the correlations of efficacy, toxicity and material basis. Consequently, blameless safely using standards should be made based on efficacy and material basis, and insure that its toxicity could be scientifically controlled. Conclusion Only study Psoralea corylifolia''s toxicity and control safely the toxic material basis during the ex

建立高效液相色谱测定补骨脂中的补骨脂素（Psoralen）和异补骨脂素（Isopsoralen）的方法。色谱条件为Ecosil C18（200 mm×4.6 mm，5μm）色谱柱、水-甲醇溶液为流动相、等梯度洗脱、波长246 nm，流速1.0 mL/min，柱温30℃。采用这种方法，补骨脂素和异补骨脂素能达到基线分离的效果，线性关系良好，具有良好的稳定性和准确度。

A method is developed for the determination of psoralen and isoporalen in Psoraea corylifolia by HPLC. The optimum condition is Ecosil C18 column, mobile phase: water-methanol solution, wavelength 246 nm, velocity 1.0 mL/min column temperature 30 ℃. This method shows a good linearity for psoralen and isopsoralen. The results demonstrate that this method is accurate and stable.

对具有耐药逆转作用的数种中药成分进行聚类分析。方法:MTT比色法测定补骨脂素、补骨脂甲素、姜黄素、槲皮素、贝母碱、异补骨脂定、补骨脂酚、大黄酸八种中药成分HL60/HT细胞的增殖抑制作用,流式细胞仪测定细胞内化疗药物HT荧光强度及细胞P糖蛋白表达;测定得到的相关数据,通过聚类分析方法进行药物分组。结果:8种药物的逆转倍数在6.2～12.5之间,HL60/HT细胞中HT浓度在9.6～25.6之间,HL60/HT细胞P-gp表达率在29.3～50.1之间。结论:基于4种实验指标可将八种药物按相似性聚类分成3组:补骨脂素、姜黄素、槲皮素3种成分为第一组,补骨脂甲素、贝母碱、异补骨脂定、大黄酸四种成分为第2组,补骨脂酚为第3组。

AIM:To do cluster analysis on the herb active ingredients with reversal activity of multi-drug resistance in HL60/HT cells.METHODS:HL60/HT cell was used to check the inhibitory effect of Psoralen,psoralen-A,curcumin,quercetin,psoralidin,isopsoralidin,psoralen phenol,Rhein on cell proliferation by MTT assay.Flow cytometry was used to measure HL fluorescence index and the expression of P-glucose protein.On the basis of the experimental data,herbs were classified as different groups by cluster analysis.RESULTS:Reversion rate,HT concentration and P-gp expression of HL60/HT fell within the range of 6.2 to 12.5,9.6 to 25.6,and 29.3 to 50.1,respectively.CONCLUSION:According to cluster similarity,8 herbal ingredients could be divided into three groups,psoralen,curcumin and quercetin were in the first group.Psoralen-A,psoralidin,isopsoralidin and rhein were in the second group,psoralen phenol was in the third group.

目的建立高效液相色谱法同时测定参茸延龄片中补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ含量的方法。方法采用Hypersil C18色谱柱（4.6 mm×250 mm,5μm）;流速：1.1 mL/min;流动相A为乙腈-甲醇（2∶1）,流动相B为0.1%冰醋酸溶液,梯度洗脱;检测波长分别为246 nm（补骨脂素和异补骨脂素）、270 nm（淫羊藿苷和宝藿苷Ⅰ）。结果补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ分别在0.033 2～0.664 0μg（r=0.999 8）、0.022 6～0.452 0μg（r=0.999 1）、0.029 4～0.588 0μg（r=0.999 5）、0.024 2～0.484 0μg（r=0.999 4）范围内进样量与峰面积呈良好的线性关系,平均加样回收率分别为97.48%、98.44%、99.13%、98.71%,RSD分别为0.92%、1.39%、1.17%、1.21%。结论本方法简便、准确、灵敏、重复性好,可作为参茸延龄片中补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ的含量控制方法。

Objective To develop an HPLC method for determination of the contents of psoralen, isopsoralen, icariin and baohuosideⅠin Shengrong Yanling Pill. Methods Hypersil C18 column was used as the chromatographic column, with gradient elution, the flow rate of 1.1 mL/min, the mobile phase A of acetonitrile-methanol (2∶1), the mobile phase B of 0.1% glacial acetic acid solution, and detection wavelength of 246 nm (for psoralen and isopsoralen) and 270 nm (for icariin and baohuoside Ⅰ). Results There was a good linear relationship between the injection volume and peak area valued when the volume of psoralen, isopsoralen, icariin and baohuosideⅠwere within the range of 0.033 2-0.664 0 μg (r=0.999 8), 0.022 6-0.452 0 μg (r=0.999 1), 0.029 4-0.588 0 μg (r=0.999 5), and 0.024 2-0.484 0 μg (r=0.999 4), respectively. The average recovery rates were 97.48% (RSD=0.92%), 98.44% (RSD=1.39%), 99.13% (RSD=1.17%) and 98.71% (RSD=1.21%), respectively. Conclusion The method was convenient, a