目的:探讨Tween对红细胞表面抗原(主要是ABO和D抗原)的遮蔽效果以及红细胞的稳定性。方法采用不同浓度的Tween孵育不同血型红细胞,并对其孵育前后抗原效价进行比较,以及采用形态观察,渗透脆性、自身溶血率、氧饱和度( SO2)、RBC平均指数检测和上清液游离血红蛋白测定判断孵育后红细胞的基本功能变化,以证实其稳定情况。结果 Tween-20对D抗原的孵育效果较好且稳定,减弱程度保持在“+”以上,B抗原遮蔽略差且并不稳定,A抗原的遮蔽效果较弱,优势浓度在0.004%附近。 Tween-80对D、B遮蔽效果差异并不明显,在0.74%、0.80%附近遮蔽效果较好,保持在“+”以上。结论 Tween-20, Tween-80可以遮蔽RBC表面抗原,并使其减弱程度保持在“+”以上,RBC形态及功能没有发生变化。
Objective To explore the shielding effect that Tween has made on the erythrocyte’ s surface antigen( ABO and D antigen mainly) and the stability of the RBC. Methods Various types red blood cells’ surface antigens were incubated with different concentrations of Tween,then the titers of RBC’ s surface antigen before and after incubation were compared. The erythrocyte’ s function alterations and sta-bility through the morphological obersavation,the osmotic fragility,RBC’ s own hemolysis rate,oxygen saturation( SO2 ) ,routine test of blood as well as the supernatant of free hemoglobin determination were confirmed. Results The masking effect of the Tween-20 on D antigen with the weaken titer keeping above“ +” was better and more stable than that on A,B antigen. The impact on B antigen was a little worse,and A was the worst. The advantaged concentration is about 0. 004%. Besides,the influence of Tween-80’s on B and D antigen was not apparent e-nough. Among all of the concen