目的制备抗人肝癌抗体与索拉非尼偶联免疫毫微球,观察其特性及抗肝癌效果。方法通过异型双功能交联剂SPDP,将抗人肝癌单克隆抗体HAb18与索拉非尼(sorafenib,SAF)人血清白蛋白毫微球[HSA(SAF)-NS]偶联,制成抗人肝癌抗体与索拉非尼偶联免疫毫微球HAb18-HSA(SAF)-NS,使用凝集试验检测其活性,光镜和电镜下观察其与人肝癌细胞株SMMC-7721特异性结合。MTT法检测该免疫毫微球的体外杀伤性。于人肝癌裸鼠模型上分别使用HAb18-HSA(SAF)-NS、HSA(SAF)-NS及SAF,检测三者的肿瘤抑制率。结果 HAb18-HSA(SAF)-NS具有单抗活性,能与肝癌细胞特异结合;其体外杀伤SMMC-7721细胞IC 50值为42.4μg/mL,与HSA(SAF)-NS(368.9μg/mL)及SAF(377.5μg/mL)相比,明显降低;体内肿瘤抑制率比HSA(SAF)-NS及SAF显著增强(P0.001)。结论 HAb18-HSA(SAF)-NS具有免疫活性,对肝癌细胞有主动靶向性,体内外均具有比HSA(SAF)-NS及SAF更强的抗癌效果。

Objective To prepare immunonanospheres containing sorafenib[HAb18-HSA(SAF)-NS] a-gainst human liver cancer and evaluate its immunoreactivity and effects on cancer. Methods HAb18-HAS (SAF)-NS was prepared by intermolecular cross-linking the monoclonal antibody HAb18 with human serum albumin nanospheres containing SAF [HAS (SAF)-NS]via a new heterobifunctional cross-linker SPDP. Con-densation test and immunofluorescence assay were used to evaluate the immunoreactivity, and the specific binding of HAb18-HSA (SAF)-NS with liver cancer cell line SMMC-7721 was observed by microscope and electron-microscope. The specific cytotoxic effects on target cells were evaluated in vitro by MTT assay. HAb18-HSA(SAF)-NS, HSA(SAF)-NS and SAF were separately injected into nude mice bearing human liv-er carcinoma to observe the inhibitory activity of HAb18-HSA(SAF)-NS in vivo. Results The immunoreac-tivity of HAb18-HSA (SAF)-NS was well preserved. Under electron-microscope, HAb18-HSA (SAF)-NS could bind SM

介绍了一款70 mDP2多用途海工船（AHTS）。重点对船型选择、DP2的软硬件配置优化、集成化设计、HAB舒适性设计方面，对整船的核心技术进行了分析研究。该船已完成生产设计，得到船东和ABS的认可，目前处在码头调试阶段。

This paper describes the core technology of 70m DP2 multipurpose offshore vessel (AHTS) by focusing on ship type, DP2 system, integrated design and HAB comfort design. Production design of the vessel has been completed and approved by ship owner and ABS, the vessel is currently in dock trial.

合成一种新型磺酸盐表面活性剂单十二烷基二苯甲烷双磺酸钠(monododecyl diphenyl methane disulfonate,以下简称MDDMDS),通过电喷雾质谱法和1H NMR确定了所合成的目标产物.考察了在NaCl和CaCl2存在下,MDDMDS与不同原油间的动态界面张力和洗油率.结果表明,在无碱条件下,MDDMDS水溶液与不同油间的界面张力在100~10-1mN/m数量级,不同质量浓度的NaCl和CaCl2对界面张力的影响不明显,与重烷基苯磺酸钠(HABS)复配后界面张力可达到10-2mN/m数量级,在高盐、高钙条件下,对石蜡基原油的模拟洗油率高于54%.

A novel surfactant, monododecyl diphenyl methane disulfonate (MDDMDS) was synthesized. The target product was verified by electrosray ionization/mass spectrometry (ESI-MS) and 1H NMR. The dy-namic interfacial tension at different oils/water interface and oil washing efficiency in NaCl and CaCl2 solutions were investigated. The results showed at alkali free environment, the oil/water dynamic interfacial tensions in the system of oil/MDDMDS surfactant were in the range of 100~10-1 mN/m orders of magnitude, and the ef-fects of different concentrations of NaCl and CaCl2 on interfacial tension were slight. The dynamic interfacial tension of the blend systems of MDDMDS with sodium heavy-alkylbenzenesulfonate (HABS) could decrease to the range of 10-2 mN/m. In high-salt and high-calcium condition, the simulated oil washing efficiency of MDDMDS for paraffinic crude oil exceeded 54%.

以铜绿微囊藻(Microcystis aeruginosa)为溶解对象,利用液体感染分离技术从浙江大学华家池校区湖水样品中分离到1株溶藻菌N10,对其生长及溶藻相关特性进行了研究.结果表明:经过16SrDNA核苷酸序列分析,该菌株属于柠檬酸杆菌属(Citrobacter sp.);当N10无细胞培养物(cell-free culture filtrate,CCF)与铜绿微囊藻液按照体积比为1∶5混合后,在24h内N10CCF对铜绿微囊藻的溶藻率可达到86.55%,并且溶藻率随着溶藻时间的延长而增强,在72h内溶藻率可达到97.08%;对N10生长量影响最大的是pH值,其次是NaCl质量分数,最后是温度,但N10菌株对铜绿微囊藻的溶藻率受培养温度和培养基中NaCl质量分数的影响很小;N10菌株通过分泌一种对高温(115℃)敏感但却能抗蛋白酶K的物质来溶解铜绿微囊藻;透射电子显微镜观察表明,经过N10CCF处理后的藻细胞出现了细胞膜、细胞壁破裂,伪空胞结构、细胞质中磷酸颗粒以及蓝色体等颗粒性物质消失,细胞中的光合片层排列趋于松散且混乱的现象.

Summary In recent decades,harmful algal blooms(HABs),which are natural phenomena that occur across all the world, have posed threats to decrease dissolved oxygen and have had a negative effect on fisheries, aquaculture,drinking water,tourism and human health.It is increasingly urgent to develop useful strategies for predicting and reducing the negative impacts of HABs.Actually,many methods have been devoted to controlling HABs,including copper sulfate,hydrogen peroxide,ozonization,ultrasonication,collection of algae from water surface using nets,iron powder and magnets,centrifugal separation,or ultraviolet radiation.As an effective and environment friendly strategy to control harmful algal bloom outbreaks,biologic control methods such as using algicidal bacteria against M.aeruginosa have been more and more attractive.It has been demonstrated that algicidal bacteria could serve as potential ways in reducing the impacts of HABs. In this study,a Citrobacter sp.N10 with algicidal activity

寄生性甲藻阿米巴藻Amoebophrya是一类广泛寄生于纤毛虫类、放射虫类、甲藻类等海水浮游生物的原生生物,在北大西洋、北太平洋和地中海等营养盐丰富、宿主密度较高的河口和近海水环境中普遍存在,是海洋浮游食物网的重要组成部分。这类寄生性甲藻能够特异性感染海洋浮游甲藻,在有害藻华(harmful algal bloom,HAB)的发生过程中起下行控制作用,将逃脱了浮游动物摄食的浮游植物补充到微食物环(microbial loop)中去。Amoebophrya在近海海洋生态系统中的独特作用日益受到国际上越来越多研究者的关注和重视,并逐渐成为国际上海洋微型生物研究的新热点之一。近年来,已有初步调查研究表明这类寄生性甲藻在我国近海海域广泛存在;然而,目前我国尚缺乏该类寄生性甲藻的相关研究。本文系统综述了国际上该类寄生性甲藻的研究进展,针对目前研究中存在的问题并结合我国有害藻华发生机制相关研究的现状做出了分析和展望,以期推动我国该类寄生性甲藻的相关研究,为进一步阐释寄生性甲藻等海洋微型生物在有害藻华消长过程和海洋微食物环中的作用奠定基础。

Parasitic dinoflagellate Amoebophrya infects broad range hosts of ciliates,radiolarians and planktonic dino-flagellates in eutrophicated coastal water worldwidely.Particularly,Amoebophrya can infect specific types of bloom-forming marine dinoflagellates,and effectively recycle undergrazed planktonic dinoflagellates back to the mi-crobial loop by disrupting host cells.The parasites play a top-down control role in their host populations and repre-sent an important aspect of marine food webs in enriched coastal environment.The ecological significance of Amoe-bophrya has been recognized in recent years by researchers around the world.Lately,scattered evidences indicated that this type of parasitic dinoflagelaltes is widely distributed along the coastal areas of China;however,few studies had been conducted on Amoebophrya in China despite that fruitful research on harmful algal booms.To promote further researchs on fundemental aspects and ecological significance of the parasitic dinoflagella