通过石蜡切片-HE染色技术,系统研究小鼠生后不同发育时期即3,5,7,10,15,20,30,45 d卵巢的卵泡分布、形态结构及发育规律.研究结果表明,随着小鼠卵巢的发育,由最初的无皮髓质之分到皮髓明显可见,卵泡从只见聚集呈条索状到发育成原始卵泡、初级卵泡、次级卵泡,直至有腔的形成.从数量上看,原始卵泡逐渐减少,初次级卵泡逐渐增多,有腔卵泡数量也从无到有从少到多.卵巢由最初的无卵泡发育到有许多大小不等、处于不同发育时期的卵泡、大部分有腔样结构.在此发育过程中会伴有卵泡的闭锁.小鼠卵巢中各级腔前卵泡的分布具有区域性.

In this paper,we investigated follicle distribution,morphological structure and rule of development of ovary at postnatal 3,5,7,10,15,20,30,45 days of ages using by paraffin section-HE staining method. The results showed that with the development of mouse,ovary develops from non-cortex and medulla assigned in the initia to the situation that cortex can be seen apparently. Follicles developed from the streak were gathered so as to develop into primordial follicles,primary follicles,secondary follicles,until cavity formation. Judging from the numbers, primordial follicles decreased in the initial stage follicles gradually,while primary follicles and secondary follicles increased gradually,and the number of antral follicles was from scratch to less than mature follicles relatively. Developing from the initial scratch to many non-sizes at different developmental stages of follicles,the majority of ovarian follicle has cavity-like structure. The distribution of follicles in the ovaries is r

【目的】探讨在水牛中增殖细胞核抗原(PCNA)参与卵泡发生的时期及其在卵巢组织不同时期卵泡中的表达定位;【方法】采用石蜡切片结合免疫组化的方法对PCNA在卵巢组织中进行定位,利用实时荧光定量PCR(QRT-PCR)技术检测PCNA在腔前卵泡中的表达情况。【结果】免疫组化结果显示:原始卵泡中的卵母细胞能检测到PCNA的表达,但颗粒细胞并未观察到PCNA的免疫染色;初级卵泡到次级卵泡,卵母细胞和颗粒细胞中均可检测到PCNA的表达,且在颗粒细胞中的表达有增强的趋势;有腔卵泡中,颗粒细胞、卵泡膜细胞及包围卵母细胞的卵丘细胞中都能观察到很强的免疫染色。定量表达检测结果显示:PCNA在原始卵泡、初级卵泡及次级卵泡中的表达呈上升趋势,并且有显著差异(43.50333±0.138338 vs 1.633804±0.093796 vs 1±0.012219,P0.01);【结论】从初级卵泡开始在颗粒细胞中能检测到PCNA的表达,并且其表达随着卵泡的发育有明显的增强趋势,腔前卵泡中PCNA表达的QRT-PCR测定结果与免疫组化结果相一致,研究结果为阐明PCNA参与卵泡发生的分子调控机制奠定了基础。

[Objective]To explore the involvement of nuclear antigen protein (PCNA) duiring different periods of buffalo follicular genesis, the expression pattern and ovary immunohistochemistry localization was performed in the present study.[Method]The localization of PCNA was demonstrated in ovarian tissue through the method of paraffin sections with immunohistochemistry, the mRNA expression level of PCNA in preantral follicles of buffalo was determined by real-time fluorescence quantification PCR(QRT-PCR).[Result]The immunohistochemical results showed that, in primordial follicle, no remarkable staining for PCNA in granulosa cells was observed, except the staining of nuclei in oocyte. In primary to secondary follicle, positive staining in oocytes and in some granulosa cells was observed, and PCNA immunoreactivity in granulosa cells of secondary follicle was obviously enhanced than that of primary follicles.The antral follicles showed extensive PCNA labeling in the layers of granulosa and theca

针药并用治疗功能性排卵障碍,子宫内膜脱落期,卵泡期,黄体期均施以中药治疗。小卵泡症,卵泡发育不良症,滞卵泡症,运用针灸治疗。结果显示,针药并用治疗取得良好效果。

In treating functional obstruction of ovulation by acupuncture and TCM, Endometrial shedding period, follicular phase, Luteal phase were treated by TCM. Small follicle syndromes, deficiency of follicular development, lag of follicle syndrome, were treated by acupuncture. The results showed that the treatment achieved good results with acupuncture and TCM.

探讨取卵日卵泡大小对体外受精-胚胎移植(IVF-ET)中卵子成熟及其受精率、卵裂率、第3日胚胎质量及囊胚形成的影响。方法:以2011年8月至2012年8月在山东省枣庄市妇幼保健院生殖中心行IVF-ET的157个周期1665枚卵子为研究对象,将其分为大卵泡组(A组)、中卵泡组(B组)和小卵泡组(C组)。比较IVF-ET过程中各组的卵子成熟度、受精率、卵裂率、胚胎发育质量。结果:与C组比较,取卵日直径15mm的卵泡获得的卵子成熟度、卵子受精率均较高(P0.05);但取卵日卵泡大小对受精后的卵裂无影响(P0.05)。大卵泡组和中卵泡组来源卵子D3日胚胎质量显著高于小卵泡组(可用胚率,P0.05)。取卵日直径≥12mm的各类卵泡来源卵子D5、D6、D7日可冻囊胚形成率无显著差异(P0.05)。结论:IVF-ET中,获得取卵日直径15mm卵泡的卵子,有利于卵子的成熟、受精,同时D3日胚胎发育质量;部分12~14mm的卵泡来源的卵子在IVF-ET术中仍具有使用价值。

Objective:To investigate the effects of different size of follicles on oocyte maturation,fertility rate,cleavage rate,embryo quality in the 3rd day and blastocyst formation. Methods:We analysed 1665 oocytes from 157 cycles of IVF-ET in our unit. All of the oocytes were classified into three groups ( the group of large size follicles,the group of middle size folli-cles and the group of small size follicles) according to the diameter size of folliculars where they come from. The oocyte maturation,fertility rate,cleavage rate,embryo quality and blastocyst for-mation of three groups in IVF-ET cycles were analysed. Results:Comparing with the oocytes coming from follicles larger than 15 mm mature better ( P 15 mm on the day of oocyte retrieval were much higher (P 0. 05). The quality of embryo originated from the group of large size follicles and the group of middle size follicles were much better than that of embryo from the group of small size follicles ( P 0 . 05 ) . Conclusion:In the IVF-

分析人绒毛膜促性腺激素(HCG)注射日最大卵泡直径及主导卵泡群比例对体外受精-胚胎移植(IVF-ET)结局的影响,以探讨HCG扳机的超声标准。方法:回顾分析2010年6月至2012年6月在河南省人民医院生殖所接受IVF-ET治疗的患者共2566周期,根据HCG日最大卵泡径线(D)分为:A组(D≤18mm)、B组(18mmD≤21mm)、C组(21mmD≤24mm)、D组(24mmD≤27mm)和E组(D27mm),比较5组的年龄、基础FSH、HCG日E2、HCG日≥14mm卵泡数、HCG日主导卵泡比例、2PN数、可移植胚胎数、胚胎着床率、临床妊娠率等。结果:A、B组HCG日≥14mm卵泡数、HCG日≥20mm比例及HCG日≥18mm比例显著低于其余3组,A组显著低于B组,差异均有统计学意义(P0.001):A、B组的HCG日≥16mm比例、胚胎着床率、临床妊娠率均低于C、D、E组,差异有统计学差异(P0.001)。5组患者的年龄、基础FSH、卵子数、2PN数、可移植胚胎数、早期流产率等比较,差异均无统计学意义(P0.05)。结论:HCG日扳机的超声标准应以主导卵泡群比例为主要参考指标并兼顾最大卵泡直径,各主导卵泡群的比例:HCG日≥20mm卵泡比例35%、≥18mm卵泡比例55%~60%、≥16mm卵泡比例80%;最大卵泡直径21mm。

Objective:To analyze the diameter of largest follicular and the proportion of dominant follicle group on human chorionic gonadotropin ( HCG) injection day on clinical out-comes of in vitro fertilization-embryo transfer( IVF-ET) ,to investigate the ultrasound standards of HCG trigger. Methods:A total of 2566 cycles IVF-ET from June 2010 to June 2012 in our reproductive center were evaluated retrospectively in the study. Cycles were divided into five groups according to the diameter of largest follicular on HCG day:group A ( D≤18 mm) ,group B(18 27mm),The age,bFSH,E2 level on HCG day,the number of follicular≥14mm,the proportion of dominant follicle group,2PN,available embryos,clinical pregnancy rate of the IVF-ET were analyzed comparatively. Results:The follicle( diameter≥14mm) number and the proportion of dominant follicle(diameter≥20mm and diameter≥18mm) on HCG day in group A and B were significantly lower than the other four groups(P 0 . 05 ) . Conclusion:The principal