目的：构建人乳腺癌中雌激素受体B（ERβ）3种亚型ERβ1、ERβ2和ERβ5的真核表达载体，测定不同亚型ERβ的转录活性。方法：以乳腺癌细胞MCF7的cDNA为模板，PCR扩增ERβ1、ERβ2和ERβ5万基因，分别克隆到pXJ40-Mye载体，Western印迹检测克隆载体在293T细胞内的表达；将上述载体与含雌激素应答元件（ERE）的萤光素酶（Luc）报告基因载体（ERE—luc）共转293T细胞，测定各亚型ER／3的转录活性。结果：构建了Myc—ERβ1、Myc—ERβ2和Mye—ERβ5表达载体，转录活性结果显示上述表达载体均具有活性，雌激素可升高ERβ5的转录活性，不能升高Eβ2和ER5的转录活性。结论：该研究为进-步探讨不同亚型ERβ在乳腺癌中的功能奠定了基础。

Objective: To construct eukaryotic expression vectors and to detect transcriptional activities of three isoforms of human estrogen receptor β(ERβ) gene containing ERβ1, ERβ2 and ERβ5 expressed in breast cancer. Methods: ERβ1, ERβ2 and ERβ5 genes were amplified from cDNA of MCF7 breast cancer cells using PCR and inserted into the vector pXJ40-Myc. Western blot analysis was used to detect the expression of these isoforms. Transcriptional activities of ERβ1, ERβ2 and ERβ5 were assayed by cotransfected these vectors and the luciferase reporter vector containing the estrogen-responsive element(ERE) into 293T cells. Results: Myc-ERβ1, Myc-ERβ2 and Myc-ERβ5 were successfully constructed as active forms. Estrogen could enhance activity of ERβ1 but not that of ERβ2 and ERβ5. Conclusion: This study could be a basis to further detect functions of different isoforms of ERβ in breast cancer.

目的探讨β绒毛膜促性腺激素（β-HCG）及其游离β亚单位（fβ-HCG）在不良妊娠中的诊断价值。方法运用化学发光分析技术分别检测早期不良妊娠患者（先兆流产组、稽留流产组、异位妊娠组）及正常妊娠孕妇（对照组）的血清β-HCG、fβ-HCG、黄体酮水平,计算各组48h后β-HCG的倍增率,对结果进行分析。结果与先兆流产组相比较,稽留流产组和异位妊娠组的β-HCG、fβ-HCG、黄体酮水平及β-HCG倍增率差异均有统计学意义（P〈0.05）。稽留流产组与异位妊娠组之间各指标差异则无统计学意义（P〉0.05）。结论β-HCG和fβ-HCG水平变化可反映不良妊娠。

Objective To explore the diagnostic value of β‐human chorionic gonadotropin (β‐HCG) and its free β‐subunit (fβ‐HCG) of adverse pregnancy .Methods Chemiluminescence technique was used for the detection of serum levels ofβ‐HCG ,fβ‐HCG and progesterone in women with early adverse pregnancy (threatened abortion group ,missed abortion group and ectopic pregnancy group) and normal pregnant women (control group) ,respectively .The 48 h doubling rate ofβ‐HCG of each group was calculated . And the results were analyzed .Results Comparing with threatened abortion group ,there were significant differences in the levels of β‐HCG ,fβ‐HCG ,progesterone and doubling rate both of missed abortion group and ectopic pregnancy group (P 0 .05 ) . Conclusion T he changes of β‐HCG and fβ‐HCG can reflect adverse pregnancy .

目的分析成人地中海贫血常见基因型患者葡萄糖6-磷酸脱氢酶(G6PD)酶活性改变情况,探讨G6PD酶活性改变在地中海贫血诊断的意义。方法用速率法检测G6PD酶活性,用GAP-PCR法检测α地中海贫血基因,反向点杂交(RDB)法检测β地中海贫血基因。结果不同基因型地中海贫血其G6PD酶活性增高不同。与正常人群平均值相比,其中α地中海贫中静止型增高1.1倍,轻型增高1.3倍,中间型(血红蛋白H病)增高1.8倍。β地中海贫血中IVS-II-654/βA、βCD17/βA、βCD71-72/βA、βCD41-42/βA四种突变类型其G6PD酶升高增高1.5倍,β-28/βA、β26/βA(血红蛋白E病)基因突变类型其G6PD酶活性增高1.2倍。结论成人地中海贫血患者的G6PD酶活性明显增高,G6PD酶活性增高可作为地中海贫血辅助诊断的参考指标。

Objective Through analyzing variety of adult G6PD enzyme activity among common thalassemia gene result, to find a relationship between thalassemia and G6PD enzyme activity. Methods G6PD enzyme activity result was tested with kinetic rate method, and the α thalassemia and β thalassemia samples were confirmed by GAP-PCR or reverse dot blotting method. Results The enzyme activity range of G6PD varied among different thalassemia genes. Compare to the normal group test result, the stationary, minor and intermediateαthalassemia group had a 1.1, 1.3 and 1.8 fold increase respectively, and the gene type ofβIVS-II-654/βA, βCD17/βA, βCD71-72/βA, βCD41-42/βA amongβ thalassemia had a 1.5 fold increase, the gene type of β-28/βA, β26/βA among β thalassemia had an increase of 1.2 fold. Conclusion G6PD enzyme activity test result among adult has a significant increase than normal group. A variety of increase G6PD enzyme activity in adult could be a reference value toαorβthalassemia.

探讨一种新的糖基转移酶β3GnT8/β3GalT7在多种肿瘤细胞中的mRNA表达情况。方法:运用半定量RT-PCR方法研究人β3GnT8/β3GalT7基因在11种恶性肿瘤细胞中的表达谱。结果:β3GnT8/β3GalT7基因在多种肿瘤细胞株中都有表达,在A549细胞中的表达最高,在SGC7901细胞中中度表达,在白血病细胞中的表达较低。结论:β3GnT8/β3GalT7基因在11种肿瘤细胞中的表达有显著差异。

Objective: To study the expression of β3GnT8/β3GalT7 in tumor cells. Methods: With RT-PCR, we detected the mRNA expression profile of β3GnT8/β3GalT7 gene in several tumor cell lines. Results: β3GnT8/β3GalT7 expressed widely in these detected tumor cell lines with different expression intension. Conclusion: It sug-gested that β3GnT8/β3GalT7 plays an important role in tumor cells.

In this paper, we construct a new Roper-Suffridge extension operator Φr n,β1,,βn(f)(z) = F(z) = ((rf(z1/r)/z1)β1z1,(rf(z1/r)/z1)β2z2,...,(rf(z1/r)/z1)βnzn)'',where f is a normalized locally biholomorphic function on the unit disc D, r = sup{|z1| : z =(z1, ···, zn) ∈Ω}, β1∈ [0, 1], 0 ≤βk≤β1, k = 2, ···, n, then we prove it can preserve the property of spirallikeness of type β, almost starlikeness of order α and starlikeness of orderα on bounded complete Reinhardt domain Ω, respectively.

In this paper, we construct a new Roper-Suffridge extension operatorΦrn,β1,···,βn(f)(z)=F(z)=rf(z1r )z1 β1z1,rf(z1r )z1 β2z2,··· ,rf(z1r )z1 βnzn′, where f is a normalized locally biholomorphic function on the unit disc D, r=sup{|z1|:z=(z1, · · · , zn) ∈ ?}, β1 ∈ [0, 1], 0 ≤ βk ≤ β1, k = 2, · · · , n, then we prove it can preserve the property of spirallikeness of type β, almost starlikeness of order α and starlikeness of orderαon bounded complete Reinhardt domain ?, respectively.