乳腺癌转移的分子机制目前尚不完全清楚。近年研究发现锌指蛋白在肿瘤形成、生长以及转移中起着重要作用，现对锌指蛋白在乳腺癌转移过程中的作用进行综述。

The molecular mechanisms of breast cancer metastasis still remain unclear.Recent studies have found that zinc finger protein plays critical roles in determining tumor formation,occurrence,development and metastasis.This review summarized the functions of zinc finger protein in breast cancer metastasis.

锌指蛋白在原核生物与真核生物基因转录的调控中发挥着重要作用.根据火龙果抗逆cDNA芯片中的一个EST设计一对引物,采用RT-PCR克隆火龙果类锌指蛋白基因片段,生物信息学分析表明,该片段长334bp,编码1中含有109个氨基酸残基的肽链,命名为HuZF.该基因序列与其它植物锌指蛋白基因核苷酸序列的同源性均在70%以上,推测的氨基酸序列与其它植物锌指蛋白的氨基酸序列的同源性达60%以上.RT-PCR分析表明,高盐和干旱胁迫下,火龙果茎中HuZF的表达增强,低温胁迫时,HuZF的表达先减弱后增强,初步推测该基因的表达与火龙果低温、高盐、干旱等逆境胁迫响应相关.

Zinc finger proteins play important roles in regulating gene expression in both prokaryotes and eukaryotes .In the presnt study ,a pair of primers was designed based on EST from cDNA microarray of pitaya (Hylocereus undulatus Britt) ,and a zinc finger protein gene fragment was cloned by reverse tran-scription-polymerase chain reaction (RT-PCR) .Bioinformatics analysis showed that the zinc finger protein gene fragment contained 334 bp nucleotide acids ,encoding a peptide chain of 109 amino acids ,and was designated as H uZF .Homology comparison with the sequences of zinc finger protein genes from other plants showed that they shared over 70% nucleotide sequence and over 60% amino acid sequence .RT-PCR analysis demonstrated that the expression of H uZF was up-regulated as exposed to high salt and drought stress ,and first down-regulated and then up-regulated under cold stress .It is tentatively speculated that the expression of H uZF is associated with the response of the plant

锌指蛋白是一类重要的转录因子,在植物应对外界生物和非生物胁迫的过程中起着极为重要的调节作用。基于二倍体雷蒙德氏棉基因组数据库,本研究从抗枯萎病、耐黄萎病的转基因抗虫棉品种鲁棉研32号中克隆到棉花锌指蛋白类基因Gh CHP。序列分析表明,Gh CHP基因的开放阅读框长度为720 bp,编码239个氨基酸,预测的蛋白质等电点为9.12。通过与其它植物锌指蛋白的序列进行聚类和比对分析,发现Gh CHP与拟南芥的AT2g16050和小麦的Ta CHP极为相近,其中两个保守的C1结构域呈典型的锌指结构。实时荧光定量PCR检测表明,Gh CHP在经过不同胁迫处理的棉花根和叶中的表达模式存在差异。PEG、Na Cl、H2O2、低温和黄萎病菌侵染处理均对Gh CHP的表达产生较大影响,表明Gh CHP可能在棉花生物和非生物胁迫响应中起重要的调控作用,是棉花品种抗逆性分子改良的重要候选基因。

Zinc finger proteins are important regulatory factors involving in plant responses to biotic and abiotic stresses. Based on genomic sequences of diploid cotton Gossypium raimondii, we cloned a new cotton zinc finger gene GhCHP from Lumianyan32, a transgenic Bt upland cotton variety characterized by Fusarium wilt resistance and Verticillium wilt tolerance. Sequence analysis revealed that the open reading frame is 720 bp by length, encoding a protein of 239 amino acids with an estimated isoelectric point of 9.12. Compared to other zinc finger protein sequences, GhCHP shows highest similarity to AT2g16050 in A rabidopsis thaliana and TaCHP in Triticum aestivum, with two highly conserved C1 domain. Further analysis by real time quantitative PCR demonstrated that the expression patterns of GhCHP in cotton leaves and roots were different under stress conditions such as PEG, NaCl, H2O2, low temperature and the defoliating Verticillium strain VD8, respectively, suggesting that GhCHP

CCCH型锌指蛋白是一类生物体中广泛存在的转录因子，与植物的抗逆性密切相关。水稻CCCH锌指蛋白家族包括67个成员，分为8个亚家族。本研究采用实时定量PCR技术分析水稻CCCH亚家族Ⅰ基因的组织表达模式以及盐、干旱、低温、高温等多种非生物胁迫和脱落酸（ ABA）对CCCH基因表达水平的调节。结果表明，多数 CCCH 基因都不同程度地受到这些非生物胁迫及 ABA 的诱导或抑制，其中 C3H10、C3H24、C3H33、C3H37和C3H67的表达量在不同胁迫处理后表现出不同程度的升高，C3H35、C3H50和C3H52在盐、干旱、ABA、低温和高温处理后表达水平呈下降趋势，表明这些CCCH锌指蛋白可能参与水稻的非生物胁迫响应。

CCCH-type zinc finger proteins , a kind of widely distributed transcription factors , are close-ly related to plant tolerance to stress .CCCH-type zinc finger protein family in rice contains 67 members, which are devided into 8 subfamilies .The tissue-specific expression patterns of CCCH -zinc finger protein subfamily Ⅰgenes and the adjustment of ABA and abiotic stresses such as salt , drought , low and hight tem-perature to CCCH genes expression level were analyzed by real time PCR .The results showed that most of CCCH genes were induced or restrained by the abiotic stresses and ABA at varying degrees .The expression quantities of C3H10, C3H24, C3H33, C3H37 and C3H67 genes were up-regulated, while those of C3H35, C3H50 and C3H52 genes were declining .It indicated that CCCH subfamily Ⅰgenes might be involved in re-sponse to abiotic stress .

基因转录调节是植物对非生物胁迫适应机制的一个重要方面,转录调节因子在胁迫信号转导途径中调节下游基因的表达,在建立植物对胁迫适应性过程中起到重要作用.锌指蛋白是功能多样的转录调节因子蛋白家族,家族成员在植物响应非生物胁迫方面扮演着重要角色.本研究以秋茄C2H2型锌指蛋白编码基因KcZFP为目的基因,在烟草中过表达KcZFP,分析C2H2型锌指蛋白在植物耐盐性中的作用.研究结果显示:转基因株系中,KcZFP表达量显著提高.过表达KcZFP的烟草植株的耐盐性明显提高,在200 mmol/L NaCl处理的条件下,KcZFP过表达烟草中脯氨酸水平远高于野生型植株.对光合作用参数比较分析显示,在KcZFP过表达植株中净光合速率受盐胁迫的影响小于野生型植株,光合系统在一定程度上得到了保护.研究结果说明KcZFP作为转录调节因子参与了植物的渗透调节,对植物的耐盐性具有贡献.

Transcriptional regulation is one of the important factors in plant adaption to abiotic stress. In stress signaling pathway, transcription factors regulate downstream genes and play a critical role in the plant acclimation to adverse environments. Among transcription factors involved in abiotic stress response, zinc finger proteins represent a large protein family which displays diverse roles in various biological processes. In this study, the function of a KcZFP gene, encoding a Cys2/His2-type zinc-finger protein of Kandelia candel, was investigated in tobacco plants under salt treatment. The results showed that the transcript abundance of KcZFP was markedly elevated in transgenic plants. Salinity tolerance of KcZFP-transgenic plants was tested under 200 mmol/L NaCl. KcZFP-overexpressed tobacco plants exhibited an obviously higher salt tolerance than that of the wild type. The proline concentration in leaves of transgenic plants was markedly higher than that in wild-type under 200 mmo