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双语推荐：高糖

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探讨L-阿拉伯糖对正常及高糖高脂饲喂昆明小鼠血糖代谢的影响。根据空腹血糖和体重筛选56只昆明小鼠随机分为7组，分别以普通饲料和高糖高脂饲料饲喂，同时用L-阿拉伯糖进行干预，观察实验第42天和第60天小鼠的空腹血糖和糖耐量水平。实验第42天和第60天，高、中剂量实验组的糖耐量水平与模型对照组比较均有统计学意义（P＜0.05或P＜0.01）。无论糖耐量检测时是否按给药剂量口服L-阿拉伯糖，一定剂量时，L-阿拉伯糖都能明显增强小鼠糖耐量水平，但在空腹血糖方面作用不明显。
We investigated the effect of the L-arabinose on Maintaining the Glucose Metabolism in mice with Normal Feeding and High-Sucrose and High-Fat Feeding. The 56 Kunming mice were divided into seven group by the limosis sera-glucose and body weight.These mice were fed with regular fodder or high glucose and fat fodder respectively and interfered in by L-arabinose at the same time. The levels of the limosis sera-glucose and glucose tolerance at 42 d and 60 d were screened. At 42 d and 60 d , the glucose tolerance in high-and mid-dose group have significance higher then the control group(P<0.05 or P<0.01). Whether the oral administration manner of L-arabinose was normal or not when we detected the glucose tolerance , a certain dose of L-arabinose was able to enhance the level of glucose tolerance.

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在栽培条件一致的情况下，对14个甘蔗品种在东方红农场进行了品比试验，测定了农艺性状、产量、糖分、抗逆性等指标。结果表明：粤糖93-159、ROC16、桂糖21号呈特早熟高糖高产；粤糖00-236呈早熟特高糖、产量较高；ROC22呈早中熟高糖高产；ROC25呈中早熟中糖高产。以上优良品种比较适宜东方红农场种植。
Comparative test of 14 varieties was conducted on Dongfanghong Farm under the same cultivation conditions, and their agronomic characters, cane yield, sucrose content and adversity tolerance were tested. As a result, Yuetang93-159, ROC16 and Guitang21 showed special early-maturity with high cane yield and high sucrose content, Yuetang00-236 showed early-maturity with high cane yield and specially high sucrose content. ROC22 and ROC25 also showed good performance in maturity, cane yield and sucrose content. These 5 varieties seemed suitable to be cultivated in Dongfanghong Farm.

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为进一步持续提高江西黎川基地烟叶质量,通过对其烟叶质量的鉴定与分析,初步明确了黎川基地烟叶质量主要特点,结果显示,下部烟叶表现为高糖、高钾、高糖碱比和低烟碱,中部烟叶表现为高糖、高钾、高糖碱比和中烟碱,上部烟叶表现为中糖、高烟碱、高钾的特性。香型风格以浓透清香为主,烟叶质量比较稳定,同年度不同地点样品或不同年度的样品差异不大,以烟碱为主及其派生的质量指标稍偏高。
for further continued improve Jiangxi lichuan base tobacco quality, through tobacco quality of identification and analysis, preliminary clear has lichuan base tobacco quality main features for: performance for the high rate of sugar and nicotine, high potassium, high sugarand low-nicotine in lower tobacco leaves, cutter tobacco leaves to the high rate of sugar and nicotine, high potassium, high sugar and medium-nicotine, upper leaves appear as properties of medium sugar, high nicotine and high potassium. Aromatic style dominated by dense fragrance;lichuan tobacco leaf quality is relatively stable, with the annual samples at different locations or different samples of the year difference is small, slightly dominated by nicotine and its derived quality indicators.

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研究不同浓度的薏苡仁油对体外高糖条件下培养的人视网膜血管内皮细胞(HRCECs)增殖及血管内皮生长因子(VEGF)表达的影响。方法:将新鲜人眼球提取的HRCECs进行体外培养,最终用于实验的为生长良好的第3~4代细胞,实验分为空白对照组、低糖对照组、高糖对照组,高糖+不同浓度(50μL/mL,100μL/mL,200μL/mL)薏苡仁油组。不同浓度的薏苡仁油对体外培养的HRCECs增殖的抑制作用是通过用噻唑蓝比色法(MTT)检测。各分组HRCECs中VEGF的表达由免疫细胞化学法检测。结果:MTT比色法结果显示:不同浓度的薏苡仁油作用于体外培养的HRCECs 48h,其细胞增殖抑制与高糖对照组相比有显著性差异(P0.05)。48h内呈浓度依赖性。而低糖对照组与高糖对照组差异无统计学差异(P0.05)。免疫细胞化学检测法表明:用50,100,200μL/mL的薏苡仁油作用于高糖下HRCECs 48h,高糖+不同浓度薏苡仁油组与高糖对照组相比VEGF表达下降明显(P0.05),将高糖+不同浓度薏苡仁油组之间进行两两比较亦具有统计学意义(P0.05)。且呈浓度依赖性。高糖对照组与低糖对照组相比,VEGF表达明显(P0.05)。结论:薏苡仁油可抑制高糖环境下HRCECs的增殖和VEGF的表达。
AlM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells ( HRCECs ) proliferation and vascular endothelial growth factor ( VEGF) expression in high glucose environment. METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd ~ 4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose + ( 50ü L/mL, 100ü L/mL, 200ü L/mL ) different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression. RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group (P 0. 05). lmmunocytochemical assay showed that:50ü

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目的：探讨高浓度胰岛素对不同糖浓度下体外培养的视网膜色素上皮（ RPE）细胞活性氧（ ROS）生成的影响。方法：体外培养人RPE（ARPE-19），随机分成空白对照组（葡萄糖5．6 mmol· L-1）、高胰岛素组（葡萄糖5．6 mmol· L-1＋胰岛素104 U· L-1）、高糖组（葡萄糖30 mmol· L-1）、高胰岛素＋高糖组（葡萄糖30 mmol· L-1＋胰岛素104 U· L-1）4组，正常糖（5．6 mmol· L-1）或高糖（30 mmol· L -1）浓度下培养72 h，再按分组给予104 U· L-1胰岛素干预24 h后，以2′，7′-二氯荧光素二乙酸酯（ DCFH-DA）法检测RPE细胞中ROS的表达量。结果：高胰岛素组、高糖组和高胰岛素＋高糖组ROS的产生量均明显多于空白对照组（t＝24．0、4．203、19．562，P＜0．05），高糖联合高胰岛素组RPE细胞ROS表达最高。结论：高浓度胰岛素促进RPE细胞ROS生成增加，可能是临床上胰岛素强化治疗导致糖尿病视网膜病变恶化的原因之一。
Objective:To investigate the effect of high dose insulin on the reactive oxygen species (ROS)expression in cultured human retinal pigment epithelial (RPE) cells under normal and high glucose condition .Methods:Human RPE cells were cultured in vitro and divided into four groups:control group(gluocose 5.6 mmol· L-1 ), high dose insulin group (gluocose 5.6 mmol· L-1+insulin 104 U· L-1 ), high glucose group(glucose 30 mmol· L-1 ), high dose insulin and high glucose group ( gluocose 30 mmol · L-1 +insulin 104 U · L-1 ).Then ROS changes were detected with flow cytometry . Results:Compared to the control,the ROS expression increased in treated groups (t=24.0,4.203,19.562 respectively,P<0.05).Conclusion:The higher expression of ROS in cultured human RPE cells by high dose insulin combined with high glucose may be one of the reasons of the deterioration of the diabetic retinopathy caused by intensive insulin therapy .

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目的观察高浓度葡萄糖对雪旺细胞增殖及凋亡的影响并初步探讨其可能机制。方法将体外培养的RSC96雪旺细胞分为正常对照组(25 mmol/L)和高糖组(100 mmol/L),分别应用M TT及流式细胞技术观察高糖对RSC96细胞增殖及细胞凋亡的影响。应用Western blotting观察高糖对Lipin-1、神经生长因子(NGF)蛋白表达的影响,并应用免疫荧光技术进一步验证在高糖刺激下Lipin-1蛋白表达变化。结果与正常对照组比较,高糖组对RSC96细胞增殖具有显著抑制作用(P0.05)。与正常对照组晚期凋亡率(1.56±0.72)%相比,高糖组细胞晚期凋亡率(12.40±0.65)%明显增加。高糖显著降低Lipin-1和NGF蛋白表达(P0.05)。结论高糖显著抑制雪旺细胞增殖并促进细胞凋亡;同时显著降低了Lipin-1和NGF的蛋白表达。提示高糖对神经细胞的损伤作用可能存在多种机制。
Objective To observe the effects of high glucose on the proliferation and apoptosis of rat RSC96 Schwann cells, and to explore the possible mechanisms.Methods RSC96 Schwann cells cultured in vitro were divided into con-trol group (25 mmol/L) and high glucose group (100 mmol/L), which were treated with high glucose as indicated. Cell proliferation was determined by methylthiazolyl tetrazolium ( MTT) staining.Cell apoptosis was assessed by flow cytometry.The protein expressions of Lipin-1 and nerve growth factor( NGF) were detected by Western blotting, and the expression of Lipin-1 was further evaluated by immuno-fluorescence.Results High glucose significantly inhibited RSC96 cell proliferation and increased cell apoptosis, which was accompanied by reduced protein expressions of Lipin-1 and NGF (P<0.05).Conclusion High glucose has an inhibitory effect on rat Schwann cell proliferation and induces cell apoptosis.Such effects are closely associated with the downregulation of Lipin-1 and NG

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目的研究冬凌草甲素（oridonin）对高糖高脂诱导的人脐静脉内皮细胞（HUVECs）炎症反应的影响。方法体外培养HUVECs，将细胞分为空白对照组、高糖高脂组（给予33μmol／L葡萄糖＋500μmol／L棕榈酸作用48h）、高糖高脂＋2．5μmol／L oridonin预处理组（给予2．5μmol／Loridonin预处理后，给予高糖高脂处理，以下简称2．5μmol／Loridonin组）、高糖高脂＋5μmol／L oridonin预处理组（给予5μmol／L oridonin预处理后，给予高糖高脂处理，以下简称5μmol／L oridonin组）。酶联免疫吸附法（ELISA）检测上清液中肿瘤坏死因子α（TNF—α）、白介素6（IL-6）的浓度，以观察细胞的炎症情况。结果高糖高脂组HUVECs细胞上清中炎症因子TNF—α、IL-6浓度明显高于空白对照组（t=14．77、14．05，P均〈0．01）。与高糖高脂组相比，2．5μmol／L oridonin组、5μmol／L oridonin组HUVECs细胞上清中炎症因子TNF-α（t=-4．81、-9．07，P均〈0．01）及IL-6（t=-4．23、-10．06，P均〈0．01）的浓度明显下降。结论冬凌草甲素能抑制高糖高脂诱导的HU—VECs细胞炎症反应。
Objective To investigate the effects of oridonin to inflammation factors of human umbilical vein en-dothelial cells( HUVECs)induced by high glucose and high fat. Methods The model of HUVECs was established and divided into 4 groups:control group,high glucose and high fat group(33 μmol/L glucose and 500 μmol/L,48 h),2. 5μmol/L oridonin group and 5μmol/L oridonin group. The concentration of tumor necrosis factor-α( TNF-α)and interleukin-6(IL-6)in the supernatant were detected by enzyme-linked immunosorbent assay(ELISA)to observe the inflammation state. Results The concentrations of TNF-α as well as IL-6 in high glucose and high fat group were significantly higher than those in control group(t=14. 77,14. 05,P<0. 01). The concentrations of TNF-α(t=-4. 81,-9. 07,P<0. 01)as well as IL-6(t= -4. 23,-10. 06,P<0. 01)in 2. 5 μmol/L oridonin group and 5μmol/L oridonin group were significantly lower than those in high glucose and high fat group. Conclusion Oridonin can inhibit i

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目的：探讨TFP5对高糖诱发的细胞周期素依赖性激酶5（Cdk5）活性的抑制作用及其对胰岛β细胞的保护作用。方法体外培养小鼠胰岛细胞株Min6。将TFP5转染Min6细胞，分别用免疫印迹和免疫荧光观察其表达。实验分3组：低糖（5mmol/L）组，高糖（25mmol/L）＋空病毒载体（EV）组和高糖（25mmol/L）＋TFP5组，分别用同位素标记法测定3组细胞内Cdk5激酶活性；用酶联免疫吸附法测定3组细胞胰岛素分泌水平；用免疫印迹法测定胰岛β细胞凋亡。结果（1）表明TFP5来源和它的分子序列；（2）TFP5在Min6细胞内表达良好；（3）在高糖＋TFP5组Cdk5的活性明显低于高糖＋EV组（ P＜0.01），而且在高糖＋TFP5组胰岛素分泌明显高于高糖＋EV组（P＜0.01）；（4）在高糖组Min6细胞的Bax表达增加，Bcl-2表达减少，Bax/Bcl-2比值升高，细胞凋亡增加（P＜0.01，vs 低糖组），而加入TFP5后，Bax表达减少，Bcl-2表达增加，Bax/Bcl-2的比值减低（P＜0.01，vs 高糖组），细胞的凋亡减少。结论 TFP5可抑制高糖诱发的Cdk5激酶的过度活性、减少高糖刺激下胰岛细胞凋亡，从而恢复胰岛素分泌，具有潜在的以Cdk5为靶点治疗2型糖尿病的前景。
Objective To determine the inhibitory effect of TFP5 on the hyperactivity of cyclin dependent kinase 5 (Cdk5) induced by high glucose and its protection for the pancreatic beta cells from apoptosis. Methods After TFP5 was delivered into mouse insulinoma line 6 (Min 6), the expression of TFP5 was detected in the cells by Western blotting and immunofluorescence staining. Then, the cells were treated by low (5mmol/L), high glucose (25mmol/L)+empty vector (EV), and high glucose +TFP5, respectively. Cdk5 activities were measured by immune precipitation and isotope labeling. The level of insulin secretion were detected with ELISA;and the apoptosis of Min 6 cells were analyzed by Western blotting. Results (1) Our results indicated the derivation of TFP5 peptide from p35 and its sequence. (2) TFP5 was well expressed in the Min 6 cells. (3) Cdk5 activity was significantly lower in the high glucose+TFP5 cells than in the high glucose+EV cells (P<0.01); the insulin secretion level in the high glu

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目的：评估高糖对肾组织干细胞（KSC）基本特征的影响。方法分离肾乳头处KSC，比较其在高糖（30 mmol/L）和正糖（5.6 mmo l/L）培养基的微环境下细胞形态、增殖能力、相关表型标记物的改变。结果细胞增殖检测结果提示从第4天开始高糖组细胞生长较正糖组缓慢；免疫荧光结果提示KCS经高糖处理后，纤维连接蛋白、Ⅰ型胶原、Ⅳ型胶原的合成增加；qRT-PCR检测结果显示KSC经高糖干预后，纤维连接蛋白、Ⅰ型胶原、Ⅳ型胶原mRNA 表达增高，间质标记物α-SMA、Vimentin基因表达进一步升高，促纤维化因子TGF-β、CTGF、PAI-1 mRNA表达均升高。结论 KSC在高糖的微环境中细胞增殖能力下降，促纤维化基因表达增高。
Objective To evaluate the effects of high glucose on the basic features of kidney stem cells. Methods Isolated the KSC from the renal papilla in 4 weeks-Sprague-Dawley rats, the cells were cultured in the media with high glucose (30 mmol/L) and with normal glucose (5.6 mmol/L) respectively. The changes of the cell morphology, proliferation, phenotypic markers were investigated. Results KSC grew slowly in the high glucose media. Immunofluorescence showed that, the synthesis of fibronectin, collagenⅠ and collagen Ⅳ increased after KSC cultured in high glucose media. And qRT-PCR test indicated that the expression of fibronectin, collagenⅠ and collagen Ⅳ mRNA also increased. The gene expression of mesenchymal marker α-SMA, Vimentin and fibrosis factors marker TGF-β, CTGF, PAI-1 were higher than that cultured in normal media. Conclusion In the high glucose medium, the KSC proliferation ability were decreased. The gene expression of fibrosis promote factors after KSC cultured

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探讨高糖、高胰岛素和不同浓度二甲双胍对乳腺癌MCF-7细胞增殖的影响。方法:体外培养人乳腺癌细胞株MCF-7,分别给予高糖和(或)高胰岛素及不同浓度的二甲双胍进行干预,MTT法检测MCF-7细胞的增殖情况。结果:高糖、高胰岛素、高糖高胰岛素作用不同时间均可促进MCF-7细胞增殖;不同浓度的二甲双胍干预后,MCF-7细胞的生长均受到一定程度的抑制,且随时间和浓度的增加而增加。结论:不同浓度二甲双胍可对高糖、高胰岛素作用下乳腺癌MCF-7细胞的增殖起到抑制作用,且呈时间和浓度依赖性。
Objective:To investigate the effects of high glucose, high insulin and different concentration metformin on proliferation of breast cancer cell line MCF-7.Methods:MTT method was used to detect the proliferation of MCF-7 cells after the MCF-7 cells were intervened by high glucose and/or high insulin and different concentration of metformin in vivo. Results:Highglucoseand/orhighinsulincouldpromoteproliferationofMCF-7cellsatdifferentactiontime.Afterintervened bydifferentconcentrationofmetformin,thegrowth ofMCF-7 was inhibited to acertain extentwhich increased with timeand concentration.Conclusions:Different concentration of metformin can inhibit proliferation of MCF-7 cells under the action of highglucoseandhighinsulin,whichshows timeand the concentration dependence.

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