简要介绍了GRP\GRC材料的性能与分类,结合GRP\GRC材料在建筑上的应用范围,分析了其在不同国家中建筑领域的应用情况,并总结归纳了建筑中应用GRP\GRC材料应注意的问题,为其推广应用提供参考依据。

The thesis briefly introduces the performance and classification of GRP\GRC material. Combining with the application scope of GRP\GRC material in building,it analyzes its application conditions in construction field of different countries,and summarizes matters needing attention in GRP\GRC material application in building,which has provided some guidance for its wide application.

EPS 装饰线条与传统的混凝土装饰线条、GRC 装饰线条、石膏装饰线条相比，主要有生产制作简单、现场安装方便容易、环保低碳并能比较贴合反应建筑师设计风格和理念等优点，是符合绿色环保趋势的材料和配件，在建筑工程中极具推广应用价值。

Compared with traditional concrete moldings and GRC moldings,plaster moldings,EPS decorative lines have more advantages,such as convenient installation,environmental protection,low carbon,suitable for the architects’design style and philosophy.It conforms to the trend of environment friendly material and accessories,worth popularization and ap-plication in construction projects.

粒计算(GrC)作为处理不精确、不确定、不完备信息的重要工具，其基本思想是粒化、粒的运算和粒运算结果的融合。部分覆盖是粒计算理论框架中的一种重要模型，在电脑安全、搜索引擎和客户评估等领域具有潜在应用价值。全覆盖是部分覆盖的一种特例，已有的研究是从粗糙集理论的角度开展的，这是一种一般拓扑观念下的全覆盖。本文在 pre-topology理论的框架下，从粒化、知识逼近和算子性质三个方面，首先介绍了邻域系统的相关定义，并在邻域系统基础之上提出了粒、全覆盖、全覆盖近似空间的概念；然后在全覆盖近似空间中利用已定义的粒重新诠释了一般拓扑中的内点和闭包算子，对全覆盖近似空间中的任意对象进行知识逼近，并用算例来说明；最后探究了全覆盖粒计算模型中这对逼近算子满足的基本性质，并证明了所提性质，为以后设计基于全覆盖粒计算模型的特征选择算法提供了理论基础。

Granular computing(GrC)is a powerful tool to handle imprecise,uncertain and incomplete information. Granulation of problems,computing of granules and integration of the results to granulated problems constitute its basic ideas.Partial covering is a global model in GrC,which has the potential application values in the research fields such as computer security,search engine and customer evaluation.As a special case of the partial covering(the global GrC model),the full covering had been studied from the perspective of the rough sets,which is depicted under the framework of the general topology.This article developed the granulation,knowledge approximation and the properties of approximation operators of the full covering from the perspective of GrC under the framework of pre-topology theory.Firstly,the concepts of the neighborhood system,the granule and the full covering GrC model were presented,and the full covering approximation space was also defined,and then the neighborhoo

逻辑优化是数字逻辑电路分析与设计的关键，对于降低系统复杂性，减少系统功耗和提高系统安全性有重要作用。随着数字逻辑电路规模的不断扩大，传统的理论将面临新的挑战。从知识工程角度看，逻辑优化的本质是知识约简的过程。粒计算（granular computing，GrC）是处理大规模、复杂问题的人工智能新方法。在简述现有逻辑优化算法和粒计算理论发展现状的基础上，研究了粒计算理论中的等价关系、相容关系、覆盖等知识模型以及用粒矩阵刻画的知识发现算法，指出了将其应用于大规模数字逻辑电路逻辑优化的研究方向与研究思路。

Logic optimization is the key for the analysis and design of digital logic circuits, which will decrease sys-tem complexity and system cost, and improve system security. With the dramatically increasing complexity of the circuits, the traditional logic optimization theory and methods are facing new challenges. From view of knowledge engineering, the nature of the logic optimization is the knowledge reduction. Granular computing (GrC) is an effec-tive way to deal with large-scale and complicated problems. Based on the review of current logic optimization algo-rithm and the development of GrC theory, this paper studies the knowledge model of granular computing theory, such as equivalence relation, tolerance relation, covering et al, and the knowledge discovery algorithm described by granular matrices, and points out the idea of using the knowledge model and knowledge discovery algorithm to the logical optimization of large scale digital logic circuit.

目的 应用抑制性消减杂交技术(SSH)筛选人肾癌差异表达基因,并探讨其对肾癌生物学功能和耐药的影响.方法 从人肾透明细胞癌细胞株RLC-310和正常细胞株HK-2中提取mRNA,应用SSH技术获得肾癌细胞差异表达基因文库,筛选差异表达最明显的基因.以逆转录聚合酶链反应(RT-PCR)和Western blot法验证差异表达基因的表达.构建干扰载体沉默人肾癌RLC-310和GRC-1细胞中差异表达基因SIPL1的表达,分别采用细胞计数法、四甲基偶氮唑蓝(MTr)法和裸鼠移植瘤实验检测RLC-310和GRC-1细胞的增殖能力,流式细胞仪检测细胞周期,MTT法检测对阿霉素的耐药性.结果 构建了人肾癌细胞差异表达基因文库,筛选了12种差异表达基因,其中SIPL1基因的表达差异最为明显.RT-PCR和Western blot法验证SIPL1基因在人肾癌细胞系及肾透明细胞癌组织中高表达.SIPL1 shRNA重组质粒稳定转染至RLC-310和GRC-1细胞后,可明显下调SIPL1蛋白的表达.与转染阴性对照组细胞比较,转染SIPL1 shRNA组细胞的增殖能力均明显下降(P＜0.05),肿瘤形成能力明显减弱[转染SIPL1 shRNA组和阴性对照组RLC-310细胞的裸鼠移植瘤重量分别为(0.22±0.07)g和(0.85±0.06)g,转染SIPL1 shRNA组和阴性对照组GRC-1细胞的裸鼠移植瘤重量分别为(0.32±0.07)g和(1.21±0.11)g,均P＜0.05],Go/G1期细胞的比例增加[转染SIPL1shRNA组和阴性对照组RLC-310细胞中Go/G1期细胞的比例分别为(71.13±4.58)％和(53.27±3.34)％,转染SIPL1 shRNA组和阴性对照组GRC-1细胞中Go/G1期细胞的比例分别为(73.83±3.97)％和(59.33 ±3.03)％,均P＜0.05],对阿霉素的敏感性提高(P＜0.05).沉默SIPL1基因表达可以使AKT通路失活,同时细胞周期抑制蛋白p27Kip1和p21ap1的表达增加.结论 应用SSH技术成功筛选出肾癌差异表达基因SIPL1.SIPL1基因是一个肾癌促进基因,可能成为肾癌诊断和治疗的新靶点.

Objective To screen the differentially expressed genes in human renal clear-cell carcinoma (RCC) cells using suppression subtractive hybridization (SSH),and to explore their biological function and underlying mechanism in RCC cells.Methods Total RNAs were extracted from human renal clear-cell carcinoma cell line RLC-310 and human normal renal cell line HK-2 cells,and SSH technology was used to construct a RCC cell library of differential expression genes and to screen the most differentially expressed genes.RNA interference vector was constructed to silence the expression of the differentially expressed gene SIPL1 in human renal cell lines RLC-310 and GRC-1.Proliferation index was estimated by cell counting,MTT and tumor xenograft assay.Cell cycle analysis was performed using fluorescence activated cell sorting.Drug resistance potential to adriamycin was assessed by MTT.Results A subtractive cDNA library of highly expressed genes in the RCC cells was constructed and 12 differentially e