利用ORP在线监控设备,研究了控制ORP值实现连续流部分亚硝化反应器稳定运行的可行性,并使出水水质满足厌氧氨氧化需求.结果表明,在亚硝氮与氨氮比值、温度和 pH 值恒定的条件下,反应器内 ORP 值波动主要由于 DO 浓度波动引起.在稳定的亚硝化系统中,当ORP值大于250mV左右时,反应器出水亚硝氮与氨氮比值大于2.1；当ORP值控制在150mV左右时,反应器出水亚硝氮与氨氮比值稳定在1.2~1.3之间.ORP值控制在120mV时,反应器出水亚硝氮与氨氮浓度比值为0.9~1.06.将ORP值控制在150mV时,随着进水氨氮浓度由300mg/L提高至813mg/L,反应器出水亚硝氮与氨氮比值基本维持在1.1~1.3之间.但随之增加的游离氨浓度易导致亚硝化菌活性抑制.因此,在低氧环境下ORP作为连续流部分亚硝化反应器亚硝化程度的控制指标,其灵敏度和精度明显优于DO监测设备.

Stable operation of partial nitritation reactor by using ORP online monitoring equipment and the effluent satisfied the anammox process were studied in continuous flow reactor. The results showed that fluctuation of ORP value was mainly caused by the fluctuation of DO concentration, when the ratio of nitrite and ammonia, pH and temperature were constant in the reactor. In partial nitritation system, the ratio of nitrite and ammonia was greater than 2.1when the ORP value was greater than 250mV, which didn’t satisfy the ANAMMOX process demanded. The ratio of nitrite and ammonia was oscillating between 1.2 and 1.3when ORP value was controlled at 150mV. The ratio of nitrite and ammonia was oscillating between 0.9 and 1.06when ORP value was controlled at 120mV. The results also showed that the ratio of effluent nitrite and ammonia was oscillating between 1.1 and 1.3 with the influent ammonia concentration increased from 300mg/L to 813mg/L when ORP value was controlled at 150mV. H

在常温条件下,采用Pasveer氧化沟,通过对氧化还原电位(ORP)、溶解氧(DO)、pH与进出水水质的相关性分析,发现碳氮比(C/N)、pH、ORP趋于稳定时对实验具有指示作用。实验结果表明:ORP稳定在60mV附近,好氧区溶解氧稳定在0.2~0.4之间,两者具有良好的相关性;氧化沟去除碳氮(COD/TN)值为1.77,证明污水中短程SND起主要作用。

Under the normal temperature ,correlation analysis between ORP ,DO ,PH and the influent /effluent water quality was conducted in Pasveer oxidation ditch .The experimental results show that the C/N ,pH , ORP in the process have an indicative function to the entire experiment .The outcome indicates that ORP , when keeps stable at around 60mV ,has great correlation with the level of DO in the aerobic zone ,which keeps stable between 0 .2 and 0 .4 .What’s more ,the ratio of carbon to Nitrogen ,i .e .,COD/TN is equal to 1 .77 , which proves that short-cut SND plays a main role in the wastewater treatment .

目的 探讨参附注射液对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)后脑皮质神经元内质网应激相关因子氧调节蛋白150(oxygen regulate protein 150,ORP150)、X盒结合蛋白1(X-box binding protein1,XBP1)和C/EBP同源蛋白(C/EBP homologous protein,CHOP)mRNA表达的影响.方法 新生7d的Sprague-Dawley大鼠随机分为假手术组、生理盐水对照组与参附治疗组(参附注射液,10 ml/kg,腹腔注射,1次/d,连续3d).按照造模后不同观察时间点又分为3h、6h、12 h、24 h、3d、7d6个亚组,每个亚组8只.建立新生大鼠HIBD模型.采用逆转录聚合酶链反应检测大鼠大脑皮质ORP150、XBP1和CHOP mRNA表达.结果 假手术组ORP150、XBP1和CHOP基因表达很弱.生理盐水对照组和参附治疗组ORP150、XBP1和CHOP mRNA表达在模型制作后3h即较假手术组显著性上调(P均＜0.05);XBP1和CHOP mRNA表达分别在6h和12 h时达高峰,之后均有所下降,7d时接近假手术组水平.其中,参附治疗组XBP1和CHOP mRNA表达在模型制作后12 h、24 h和3d时显著性低于生理盐水对照组(P均＜0.05);生理盐水对照组XBP1 mRNA表达与CHOP mRNA表达呈显著正相关(r=0.649,P＜0.05).结论 新生大鼠HIBD可诱发内质网应激反应,ORP150、XBP

Objective To investigate the effect of Shenfu injection on the expressions of endoplasmic reticulum stress-related factors oxygen-regulated protein 150 (ORP150),X-box binding protein 1 (XBP1),and C/EBP homologous protein (CHOP) mRNAs in cerebral cortical neurons after hypoxic-ischemic brain damage (HIBD) in neonatal rats.Methods The 7 day-newborn Sprague-Dawley rats were randomly divided into sham operation,normal saline and Shenfu treatment (Shenfu injection,10 ml/kg per day,peritoneal injection,for 3 days) groups.They were redivided into 3 h,6 h,12 h,24 h,3 d and 7 d subgroups at different time points after modeling (n =8 in each group).A HIBD model of the neonatal rats was induced.Reverse transcription-polymerase chain reaction was used to detect the expressions of ORP150,XBP1,and CHOP mRNAs in rat cerebral cortex.Results The expressions of ORP150,XBP1 and CHOP mRNAs of the sham operation group was very week.The expressions of ORP150,XBP1 and CHOP mRNAs in the normal salin

为了解上覆水环境以及生物作用对水库底泥释磷作用的影响,本研究通过选取石砭峪水库底泥作为贫营养水库底泥代表,在实验室模拟了不同物理化学条件及不同微生物条件下水库底泥静态释磷过程.实验期间调查了上覆水处溶解氧(DO)、氧化还原电位(ORP)、pH等条件的影响,定期监测了上覆水中的溶解性正磷酸盐(PO3-4)、总磷(TP)、亚铁离子(Fe2+),反应开始前与结束后测定了底泥中不同持留形态的磷组分,其中包括铁铝结合态磷(Fe/Al-P)、钙磷(Ca-P)、无机磷(IP)、有机磷(OP)、总磷(TP),同时测定了反应前后底泥中碱性磷酸酶活性(APA).实验证明,ORP0 mV的强还原性条件能够促进底泥中的磷大量释放,同时伴随着大量的Fe2+进入上覆水中.底泥中释放的磷是以Fe/Al-P和IP为主,并且进入水体中的磷大部分是PO3-4,占超过水体中TP的50%以上.底泥中微生物的活动能够促进OP的分解和转化,对底泥中其他形态的磷转化为PO3-4进入水体影响不大.同时微生物也可以吸收上覆水中除PO3-4之外的磷营养进入底泥中储存起来.

In order to understand the effects of overlying water environment and the microbial activity on the release of phosphorus ( P ) from sediments, static release processes of P in sediments of reservoir under different physical and chemical conditions and microbial activity conditions were simulated in the laboratory by selecting sediments in Shibianyu Reservior as representative of the poor nutrient reservoir. Dissolved oxygen ( DO) , oxidation-reduction potential ( ORP ) and pH value in overlying water were measured during the experiment. Soluble orthophosphate ( PO3-4 ) , total phosphorus ( TP ) and ferrous ions ( Fe2+) were regularly monitored by on-line monitoring. P of different forms including Fe-bound phosphorus ( Fe/Al-P ) , Ca-P , inorganic phosphorus ( IP ) , organ phosphorus (OP) and total phosphorus (TP) and alkaline phosphatase (APA) in sediments were measured before and after the reaction. The experiment showed that strong reducing conditions with ORP less than 0 mV promote

通过摇瓶实验,在Mg2+分别为48,4.8mg/L,其他元素组成与9K液体培养基一致的体系中,采用氧化亚铁硫杆菌A.ferrooxidans催化合成次生铁矿物.考察了Mg2+含量对生物合成次生铁矿物体系pH值、氧化还原电位(ORP)、Fe2+氧化率、总Fe沉淀率、次生铁矿物矿相及矿物晶体尺寸的影响.结果表明,经过48h培养,Mg2+浓度为48,4.8mg/L生物成矿体系pH值分别从原来的2.50降低至2.30,2.19,ORP分别从初始259mV增加至269mV,276mV.两体系Fe2+氧化率培养至第48h均达到100%,然而两体系总Fe沉淀率及矿物形态及却不尽相同.Mg2+浓度为48mg/L生物成矿体系,总Fe沉淀率为23.7%,次生矿物紧密粘附于三角瓶底部.而Mg2+浓度为4.8mg/L生物成矿体系,总Fe沉淀率达到32.2%,次生矿物却均匀分散于溶液中.两体系合成次生铁矿物均为黄铁矾与施氏矿物共存的混合物,Mg2+含量4.8mg/L体系合成黄铁矾单个晶体长度(~1.60μm)约为Mg2+含量48mg/L体系的1.2倍.

In this study, secondary iron minerals was synthesized by A. ferrooxidans in the solution with 48 or 4.8mg/L magnesium ions and other elements in consistent with 9K liquid medium. pH, oxidation reduction potential, Fe2+oxidation rate, total Fe precipitation rate, secondary iron minerals phase and mineral crystal size in two systems were investigated. pH in the treatment with 48or 4.8mg/L of magnesium ion decreased from 2.50at 0h to 2.30or 2.19at 48h, whereas ORP increased from 259mV to 269mV or 276mV, respectively. Ferrous ions could be completely oxidized by A. ferrooxidans within 48h, secondary iron minerals morphology and total Fe precipitation rate, however, were different in two systems. With 48mg/L of magnesium ion, the total Fe precipitation rate was 23.7%after 48h reaction, while it rose to 32.2% in the presence of 4.8mg/L of magnesium ion. The precipitates formed in the two treatments were a mixture of jarosite and schwertmannite. However, unlike the precipitates closely adher