目的 观察生长抑素受体-2(SSTR2)基因对肝癌细胞株MHCC-97H侵袭转移及上皮-间充质转化(EMT)特性的影响.方法 构建慢病毒3FLAG-puromycin-LV及SSTR2-LV,转染肝癌细胞株MHCC-97H,噻唑蓝(MTT)、免疫荧光检测转染效率.采用Transwell侵袭实验和迁移实验分别检测未转染细胞(空白对照组)、转染3FLAG-puromycin-LV细胞(阴性对照组)和转染SSTR2-LV细胞(实验组)侵袭转移能力的强弱.镜下观察转染前后细胞形态的变化,免疫荧光检测转染前后细胞上皮表型蛋白和间质表型蛋白的定位和表达,实时定量聚合酶链反应(Real-time PCR)检测转染后细胞SSTR2的表达,Westem blot检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)蛋白的表达.结果 SSTR2-LV可以有效转染肝癌细胞株MHCC-97H,稳定表达SSTtR2的mRNA和蛋白.转染SSTR2-LV的肝癌细胞株MHCC-97H较空白对照组和阴性对照组侵袭迁移能力明显受到抑制(P＜0.05).镜下观察肝癌细胞株MHCC-97H转染SSTR2-LV后,由成纤维细胞样、排列分散,转变为细胞排列紧密如铺路石.免疫荧光染色结果可见肝癌细胞株MHCC-97H转染后细胞膜表达的E-cadherin荧光由胞质转移至细胞周边浓聚,而Vimentin的荧光强度较前下降.采用Western blot进行蛋白定量分析,转染后MHCC-97H细胞上皮表型标志性蛋白E-cadherin、β-连环蛋白(β-catenin)表达量增高,间质表型标志性蛋白N-cadherin、Vimentin表达量减少,差异具有统计学意义(P＜0.05).结论 转染再表达SSTR2MHCC-97H细胞可以增加上皮表型蛋白表达,减少间质表型蛋白,从而逆转肝癌细胞EMT,导致肿瘤细胞侵袭能力减弱.

Objective To investigate the effect of the somatostatin receptor subtype 2 (SSTR2) on the migration and invasion of human Hepatocellular carcinoma cells MHCC-97H and the change of epithelial-mesenchymal transition (EMT).Methods Constructed 3FLAG-puromycin-LV and SSTR2-LV and transfected hunman Hepatocellular carcinoma cells (MHCC-97H) respectively.The expression of SSTR2 was detected by real-time quantitative polymerase chain reaction (Real-time PCR) and Western blot.The migration and invasion of Non-transfected (blank control group),3FLAG-puromycin-LV transfected (negative control group) and SSTR2-LV (experimental group) transfected MHCC-97H cells was detected by Transwell invasion assay and Transwell migration assay.The changes in morphology of cells were observed by optical microscopy.The localization and expression of the epithelial markers and the mesenchymal markers were assayed by immunofluorescence and Western blotting.Results The stable mRNA and protein expression of SSTR2 was

目的 探讨p38丝裂原活化蛋白激酶(p38 MAPK)和c-Jun氨基端激酶(JNK)信号通道在转化生长因子β1 (TGF-β1)诱导的人肺泡上皮细胞上皮-间质转化(epithelial to mesenchymal transition,EMT)中的意义,从而进一步揭示肺纤维化的发病机制.方法 使用TGF-β1诱导A549细胞EMT,分别在蛋白水平(使用p38 MAPK抑制剂SB203580和JNK抑制剂SP600125)和RNA水平(RNA干扰)抑制p38 MAPK和JNK信号通道,检测抑制后A549细胞的p38 MAPK和JNK的蛋白和mRNA,以及间质细胞表型蛋白包括结蛋白、波形蛋白、α-平滑肌肌动蛋白和上皮细胞表型蛋白包括E-钙黏素、紧密连接蛋白-1、水通道蛋白-5的表达.结果 TGF-β1可以诱导A549细胞EMT,表现为间质细胞表型蛋白表达的增加和上皮细胞表型蛋白表达的减少,这一过程p38 MAPK和JNK通道表达也增加,无论蛋白水平抑制或基因沉默p38 MAPK和JNK均可减轻A549细胞的EMT.结论 p38 MAPK和JNK信号通道在TGF-β1诱导的A549细胞EMT过程中起重要作用.

Objective To investigate the roles of p38 MAPK and JNK in transforming growth factor-β1 (TGF-β1)-induced human alveolar epithelial to mesenchymal transition (EMT),and to demonstrate the possible molecular mechanism of idiopathic pulmonary fibrosis.Methods A549 cells were treated with TGF-β1 (3 μg/L) for 48 hours to induce EMT.The expressions of mesenchymal phenotypic markers including desmin,α-smooth muscle actin (α-SMA) and vimentin,and expressions of epithelial phenotypic markers including E cadherin,zonula occludens-1 (ZO-1) and aquaporin-5 (AQP-5) were detected by Western blot.The role of p38 MAPK and JNK in TGF-β1-mediated EMT was investigated using gene silencing and inhibitor SB203580 and SP600125.Results The data showed that TGF-β1 induced A549 cells with alveolar epithelial type Ⅱ cell phenotype to undergo EMT.The process of EMT was accompanied by increased expression of the mesenchymal cell markers α-SMA,desmin and vimentin,and downregulation of the epithe

目的：检测HOST2 lncRNA在上皮性卵巢组织的表达水平，并分析其临床意义。方法：利用实时荧光定量PCR方法检测HOST2 lncRNA在上皮性卵巢癌、上皮性交界性瘤和上皮性正常卵巢组织的表达水平，分析HOST2 lncRNA在不同上皮性卵巢组织表达差异的临床意义。结果：HOST2 lncRNA在上皮性卵巢癌组织和上皮性交界性瘤表达量明显高于上皮性正常卵巢组织的表达，差异均有统计学意义（P0.05）。HOST2 lncRNA在不同临床分期的上皮性卵巢癌中表达量无明显差异（P>0.05）。结论：在上皮性卵巢癌和交界性卵巢瘤中HOST2 lncRNA均有表达，可作为上皮性卵巢癌早期诊断的一个分子诊断标志物。

Objective:To detect the expression of HOST2 lncRNA in epithelial ovarian carcinoma, and analyze its clinical significance.Method:The HOST2 expression of epithelial ovarian cancer , epithelial borderline tumor and epithelial ovarian normal tissues were detected by the PCR method. Clinical significance differences in the expression of HOST2 lncRNA in different ovarian tissue was analyzed.Result:Expression of epithelial ovarian cancer tissue and epithelial borderline tumor was obviously higher than expression of epithelial normal ovarian tissues,there was significant statistical difference (P 0.05).HOST2 lncRNA’s expression of different clinical stages of epithelial ovarian tumor was no significant difference(P>0.05).Conclusion:HOST2 lncRNA expression in ovarian cancer and epithelial borderline tumor, maybe a marker for early diagnosis of ovarian cancer.

探讨不同直径角膜上皮环锯对准分子激光上皮下角膜磨镶术(LASEK)的影响。方法:对264例528眼近视患者施行LASEK手术,随机分为观察组和对照组,分别使用7mm,8mm直径上皮环锯制作角膜上皮瓣。术后1,3,5d比较两组术后角膜刺激症状,术后5d比较两组角膜上皮愈合情况,术后1mo比较两组裸眼视力,术后3mo比较角膜上皮下雾状混浊(haze)程度。结果:术后1,3,5d观察两组角膜刺激症状、5d观察角膜上皮愈合情况,均明显好于对照组,比较有统计学意义(P0.05)。术后1mo两组裸眼视力、术后3mo两组haze比较差异无统计学意义(P0.05)。结论:使用较小直径上皮环锯的LASEK术后角膜刺激症状轻、上皮愈合和视力恢复较快,总体临床疗效优于使用较大直径上皮环锯。

AIM:To explore the effect of different diameters corneal epithelial trephine on laser epithelium keratomileusis METHEDS: 264 cases ( 528 eyes ) with myopia were divided into the observed group and the control group randomly, then underwent LASEK surgery using 7mm and 8mm diameters corneal epithelial trephine respectively to make corneal epithelial flap. The comparisons between groups were conducted in terms of corneal irritation, the time of the epithelium healing at 1, 3, 5d, visual recovery at 1mo, and the degree of haze at 3mo. RESULTS: Corneal irritation at postoperative 1, 3, 5d and the epithelium healing at postoperative 5d in the observed group was much better than that in the control group, there was significant difference (P 0.05). CONCLUSION: LASEK with the smaller diameter corneal epithelial trephine is a safe and efficient method to correct myopia than with the bigger diameter with the advantages of having milder irritating symptom, a shorter time of the epithelium healing

目的 探讨羊膜移植治疗反复性角膜上皮糜烂的临床疗效.方法 回顾性分析保存生物羊膜移植治疗的20例(21眼)反复性角膜上皮糜烂住院患者,术后随访观察3～16个月,对术后角膜上皮完全修复时间、视力提高情况进行分析评价.结果 21眼中19眼单纯羊膜移植术,术后角膜上皮完全修复时间最短3d,最长28 d;1眼于术后第8天羊膜融解,角膜上皮未愈合,经再次手术并联合佩戴角膜接触镜治疗后角膜上皮完全修复;1眼首次手术及联合佩戴角膜接触镜治疗,术后7d角膜上皮完全修复.术后视力提高(t=2.613,P=0.02).结论 羊膜移植是治疗反复性角膜上皮糜烂的有效方法.

Objective To study the clinical efficacy of amniotic membrane transplantation for recurrent corneal epithelial erosion.Methods We retrospectively analyzed 21 eyes of 20 cases with recurrent corneal epithelial erosion treated by amniotic membrane transplantation.The follow-up time was 3-16months.The complete repair time of corneal epithelium and the improvement of visual acuity were evaluated.Results Of the 21 eyes,19 eyes underwent amniotic membrane transplantation and the shortest repair time of corneal epithelium was 3 days,the longest was 28 days.The amniotic membrane of 1 eye was dissolved at 8 d after operation,and the comeal epithelium did not repair until reoperation of transplantation combined with wearing corneal contact lens.The comeal epithelium of the other 1 eye completely repaired at 7 d after amniotic membrane transplantation combined with wearing corneal contact lens.The visual acuity was improved after surgery (t =2.613,P =0.02).Conclusion Amniotic membrane transplanta