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双语推荐：甘油磷酸盐

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目的：揭示全自动生化分析仪使用过程中各检测项目间相互交叉污染的问题。方法以无机磷、镁和甘油三脂为对象将内含磷酸盐、镁离子和甘油类组分的检测项目和这3个项目做几种组合，观察这些试剂对病人标本检测这3个项目的影响。结果说明这3个项目的检测设置对检测结果有明显影响，差异高达数倍。讨论应注意分析项目的设置顺序，对已论证设定好的检测顺序不能随意改动。
Objective To reveal the cross contaminations of each detect items in using of automatic biochemical analyzer. Methods Three reagents including inorganic phosphate, magnesium ion and triglyceride were involved in this program. To observe the influence for patients’ samples due to different arrangements of three reagents. Results There were significantly difference to detect results according to different arrangements. Conclusions Pay attention to arrangements of analytic items, don’t change the demonstrated sequence of established items.

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采用重组大肠杆菌 Lin 43利用甘油产丙酮醇。为了改善菌株对丙酮醛的耐受性，采取敲除 glo B基因而不是gloA 基因以阻断丙酮醛的脱毒途径。实验结果表明，菌株 Lin43ΔgloB 对丙酮醛的耐受性要明显优于 Lin43ΔgloA。在含20 g?L-1的甘油磷酸盐缓冲液中，通过26 h 静息细胞转化，菌株 Lin43?gloB pCA24N-yqhE 丙酮醇产量达到2.30 g?L-1，并能耐受3.5 mmol?L-1的丙酮醛。同时 Lin43?gloB pCA24N-yqhE 实现重复发酵，第二轮发酵的产量可达1.53 g?L-1，丙酮醇的得率与第一轮相同。
Gene gloB instead of gloA in the strain of Escherichia coli Lin43 was knocked out to disrupt the detoxification pathway of methylglyoxal. The results show that the tolerability of strain Lin43ΔgloB is much better than that of the strain Lin43 ΔgloA. After resting the cells of strain Lin43 ΔgloB pCA24N-yqhE in the phosphate buffer with 20 g?L-1of glycerol, it produces 2.30 g?L-1 of acetol in 26 h and can resist 3.5 mmol?L-1 methylglyoxal. Moreover, 1.53 g?L-1 acetol can be produced with strain Lin43 ΔgloB pCA24N-yqhE in the second round production, which has similar yield as that of the first round.

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为筛选一种较好的酶标抗体稀释液,试验以磷酸盐缓冲液(PBST)为基础溶液,通过添加甘油、酪蛋白、海藻糖及防腐剂Proclin 300等试剂配制了4种不同组合的酶标抗体稀释液,分别在4℃和37℃条件下考察其对酶标抗体稳定性的影响。结果表明,处理5(0.01 mol/L PBST+3%酶稳定剂M+3%海藻糖+0.05%Proclin 300)的保护效果最好,在4℃保存6个月,酶标抗体的活性仅降低18%。
Inthisstudy,fourdifferentcombinatorialenzyme-labelledantibodydiluentspreparedwiththePBST buffersolutionasabasisto whichglycerol,casein,trehaloseor/andProclin300reagentwasaddedwereusedtoinvestigatetheeffectofthefourdiluentsonthestability of enzym e-labelled antibody at 4 ℃ or 37 ℃, respectively, in order to screen a better enzym e diluent for the enzym e-labelled antibody stability.Theresultsshow thatthetreatment5(0.01M PBST +3% enzymestabilizer+3% trehalose+0.05% Proclin300)wasoftheoptimal protective effectand could be keptat4℃for6 m on so thatthe activity ofthe enzym e-labelled antibody therein reduced by only 18%.

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为减少高硅钢铁芯叠片间的涡流损耗，以磷酸二氢铝、苯丙乳液、甘油及水为主要原料，制备出一种适合高硅钢用无铬环保半有机绝缘涂层．利用光电子谱仪、扫描电镜及能谱仪对高硅钢脱碳退火板的氧化薄膜、绝缘涂层的微观结构形貌及部分缺陷进行分析，并研究了该涂层的涂覆量对其附着性、硬度及绝缘性能的影响．研究结果表明：普通无取向硅钢磷酸盐环保半有机绝缘涂层同样适用于高硅钢；绝缘涂层的均匀性取决于高硅钢片表面的平整度、涂辊表面质量以及对涂覆速度的平稳控制；高硅钢的表面粗糙度及氧化膜厚度对绝缘涂层的附着性有着重要影响；本试验制备的半有机涂层每面涂覆量控制在0．8～1．2 g／m2，具有良好的附着性及绝缘性能，层间电阻在5Ω·cm2／片以上．
To reduce eddy current loss between the core lamination of high silicon steel, a kind of chromium?free environment friendly inorganic?organic insulation coating was prepared by Al(H2PO4)3, St-BA emulsion, glycerol and H2 O. The oxide film of decarburization annealed high silicon sheet, the microstructure and defects of the coating were analyzed by X?ray photoelectron spectroscopy(XPS), scanning electron microscope (SEM) and energy dispersive spectroscopy ( EDS ) . The effects of coating weight on the adhesiveness, hardness and insulating properties were studied. The results show that the phosphate inorganic?organic insulation coating for non?oriented silicon steel can also be suitable for high silicon steel. The uniformity of the insulating coating depends on the surface flatness of high silicon steel, the surface quality of coating roll and the controlling coating speed. The surface roughness and thickness of oxide film of high silicon steel have influences on the adhesivene

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【目的】玉米赤霉烯酮(zearalenone,ZEA)是由谷物及其产品滋生禾谷镰刀菌产生的一种具有雌激素活性的真菌毒素。试验研究不同水平玉米赤霉烯酮污染日粮对断奶仔猪血清代谢产物、肝肾组织病理学和肝脏超微结构的影响。【方法】将20头健康三元(斯格×长×大)杂交断奶母猪(10.36±1.21)kg按照体重随机分为4个处理,仔猪采用试验笼单独饲养。对照组饲喂基础日粮,试验1、2和3组分别在基础日粮的基础上添加ZEA 1.1、2.0和3.2 mg·kg-1。预饲期7 d,正式期18 d。试验结束后,对仔猪禁食12 h后进行前腔静脉空腹采血,分离血清,待测血清代谢产物含量。采血后,仔猪电击致死放血屠宰,切取肝脏和肾脏组织块迅速固定于10%的福尔马林溶液,待做组织切片检测。切取0.5 mm3大小的肝脏组织块,迅速用2.5%戊二醛磷酸盐缓冲液固定,待做电镜切片进行超微结构观察。【结果】与对照组相比,3.2 mg·kg-1 ZEA处理组仔猪血清胆红素显著高于对照仔猪(P0.05),而血清球蛋白和甘油三酯则显著低于对照组(P0.05)。随日粮ZEA水平的增加,血清球蛋白和甘油三酯呈一次线性降低(P0.05)。与对照组相比,1.1、2.0和3.2 mg·kg-1ZEA处理组肝细胞肿胀,颗粒变性。与对照组相比,2.0和3.2 mg·kg-1 ZEA处理组肾小管上皮细
[Objective]Zearalenone (ZEA) is one of estrogenic mycotoxins produced mainly by Fusarium fungi growing on grains and their derived products. The aims of the present study were to investigate the effect of ZEA on serum metabolite, histopathology of liver and kidney and liver ultrastructure in weaned piglets.[Method]A total of twenty healthy weaned gilts (Landrace × Yorkshire × Duroc) with an average body weight of (10.36 ± 1.21) kg (mean ± SD) were used in the study. Gilts were randomly allocated into 4 treatments according to body weight and fed individually in a metabolic cage for 18 days after 7-d adaptation. Piglets were fed a basal diet only (control) or basal diet supplemented with ZEA at a dietary concentration of 1.1, 2.0or 3.2 mg·kg-1. Piglets were fasted for 12 h at the end of the experimental period. After the collection of blood samples, piglets were immediately killed by electrocution and livers and kidneys were isolated. Samples of livers and kidneys from ea

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目的 探讨高镁对高磷诱导血管钙化的影响及其可能的机制.方法 体外原代培养大鼠胸主动脉血管平滑肌细胞(VSMCs),用β甘油磷酸盐(β-GP)诱导钙化.VSMCs细胞被分为4组:对照组、高磷组(10 mmol/L β-GP)、镁干预组(10 mmol/L β-GP+3 mmol/LMgSO4)、镁通道抑制剂(2-APB)干预组(10 mmol/L β-GP+3 mmol/L MgSO4+10-4 mol/L 2-APB).采用茜素红染色及邻甲酚酞络合酮比色法检测细胞钙化情况;酶联免疫吸附法(ELISA)检测细胞碱性磷酸酶(ALP)活性;RT-PCR法和Western印迹法检测细胞核心结合因子α-1(Cbfα-1)的表达.24只雄性SD大鼠被随机分为3组:对照组(甲基纤维素灌胃+高磷饮食)、血管钙化组(硫酸腺嘌呤灌胃+高磷饮食)、高镁干预组(硫酸腺嘌呤灌胃+高磷高镁饮食).造模成功后测定大鼠主动脉脉搏波速率(PWV);采用yon Kossa染色及邻甲酚酞络合酮比色法检测胸主动脉钙化情况;免疫组织化学方法检测胸主动脉Cbfα-1表达.结果 细胞培养14 d后,与高磷组相比,镁干预组VSMCs钙盐沉积明显减少,ALP活性降低(P＜0.05),Cbfα-1表达下调;2-APB可抑制高镁对VSMCs的保护性作用.Cbfα-1的动态观察结果显示,镁干预组第3天Cbfα-1的表达下调(P＜0.05),其抑制效应随时间延长呈增强趋势.体内实验中,大鼠慢性肾衰竭血管钙化模型制备成功.和体外实验一致,与血管钙化组相比,高镁干预组大鼠血浆镁离子水平明显升高,胸主动脉PWV明显降低(P＜0.05),血管钙盐沉积程度亦明显减轻.免疫组织化学结果显示高镁可明显降低高磷诱导的Cbfα-1表达(P＜0.05).结论 高镁可抑制血管钙化,其机制可能与其抑制VSMCs骨源性分化相关.
Objective To explore the effect and mechanism of magnesium on calcification induced by hyperphosphate.Methods Vascular smooth muscle cells (VSMCs) were primarily cultured in vitro and induced calcification by β-glycerophosphate (β-GP).VSMCs were randomly divided into control group,high phosphorus group (10 mmol/L β-GP),magnesium intervèntion group(10 mmol/L β-GP + 3 mmol/L MgSO4) and 2-aminoethoxy-diphenylborate (2-APB,an inhibitor of magnesium transporter) intervention group(10 mmol/L β-GP+3 mmol/L MgSO4+ 10-4 mol/L 2-APB).Calcium deposition and alkaline phosphatase (ALP) activity were measured by alizarin red staining,quantification of calcium and euzyme linked immunosorbent assay.RT-PCR and Western blotting were used to observe the expression of core binding factor α-1 (Cbfα-1) mRNA and protein,respectively.In vivo,male Sprague-Dawley rats (n=24) were randomly divided into control group (methylcellulose+high phosphorous diet),vascular calcification group (adenine s

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目的 构建周期型马来丝虫半胱氨酸蛋白酶抑制剂/3-磷酸甘油醛脱氢酶(BmCPI/BmGAPDH)复合基因重组质粒,并转染人宫颈癌细胞(Hela)进行重组蛋白表达.将重组质粒和相应表达蛋白进行免疫学研究比较,为研制新型的抗丝虫基因工程疫苗提供理论及实验依据.方法 扩增周期型马来丝虫目的编码基因.将目的基因片段和载体分别双酶切后进行连接,构建复合基因重组质粒pcDNA3.1(+)-BmCPI/BmGAPDH,鉴定后转染Hela细胞,以G418筛选转染细胞,进而通过RT-PCR和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定G418筛选后的单克隆抗性细胞株.亲和层析纯化所表达的重组蛋白并通过免疫蛋白印迹法(Western blot)对纯化的重组蛋白进行生物学鉴定.60只BALB/c小鼠分5组,每组12只,第2、4、6周分别免疫1次.磷酸盐缓冲液(PBS)对照组为注射PBS 100 μl;空质粒/CpG对照组为注射pcDNA3.1 100 μg和CpG 30 μg;复合重组质粒/CpG组为注射pcDNA3.1-BmCPI/BmGAPDH 100 μg和CpG 30 μg;复合重组蛋白/CpG组为注射pcDNA3.1-BmCPI/BmGAPDH复合重组蛋白50 μg和CpG 30 μg;复合重组质粒/复合重组蛋白/CpG组前2次注射pcDNA3.1-BmCPI/BmGAPDH 100 μg和CpG 30 μg;后1次注射pcDNA3.1-BmCPI/BmGAPDH复合重组蛋白50 μg和CpG 30 μg.用RT-PCR方法检测肌肉组织内目的基因;用酶联免疫吸附试验(ELISA)、特异性增殖试验(MTT法)分别检测免疫小鼠血清抗体效价、T淋巴细胞刺激增殖指数和血清中细胞因子干扰素(INF)-γ、白细胞介素(IL)-4水平.结果 复合重组质粒pcDNA3.1 (+)-BmCPI/BmGAPDH免疫小鼠后,从小鼠肌肉组织扩增出目的基因.复合重组质粒/复合重组蛋白/CpG组小鼠免疫4、6周后血清抗体效价(1 695.12±1.43、3 199.63±1.34)均高于复合重组质粒/CpG组(712.69±1.08、1 510.08±1.43,P均＜0.05)和复合重组蛋白/CpG组(800.02±1.34、1 510.08±1.43,P均＜0.05);第6周的复合重组质粒
Objective To construct a plasmid DNA vector expressing cysteine protease inhibitor and glyceraldehydes-3-phosphate dehydrogenase of periodic Brugia malayi(BmCPI/BmGAPDH),and purify the recombinant protein after transfecting the vector into human cervical carcinoma cells(Hela) for expression.To make a comparison of immunity efficacy between the recombinant plasmid and the homologous protein and to a lay theoretic and experimental basis for developing novel anti-filarial genetic engineering vaccines.Methods The amplified genes BmCPI and BmGAPDH and a plasmid vector were double enzymes digested and ligated to construct a recombinant plasmid pcDNA3.1 (+)-BmCPI/BmGAPDH,and this plasmid was transfected to Hela cells after being identified.G418 was used for screening transfectants,and the monoclonal resistant cell strain was determined by RT-PCR and SDS-PAGE.The recombinant protein was purified by affnity chromatography and identified by Western blotting.Sixty BALB/c mice were divided into 5

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