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双语推荐：TTC染色

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目的比较应用不同2,3,5-氯化三苯基四氮唑（TTC）染色方法对大鼠心肌缺血再灌注损伤后梗死面积的检测效果。方法将20只SD大鼠（雄性,8周龄,体重250~300 g）按随机数字表法分为两组,每组10只。A组：传统TTC染色法组;B组：改进后的TTC染色法组,分别进行大鼠心肌染色,随后计算心肌梗死面积及测定血清cTnI浓度水平。结果 A组和B组均能较好地标记梗死心肌;A组和B组心肌梗死面积百分比无统计学差异（48.69%±5.37%vs.47.41%±3.28%,P〉0.05）;A组和B组血清cTnI浓度水平无统计学差异（4.51±0.88 ng/mL vs.4.70±0.71 ng/mL,P〉0.05）;但B组心肌切片染色色泽对比度及心肌非梗死区与梗死区区分度均高于A组。结论改进后的心肌TTC染色法采用在体染色,不仅操作简便,节省了实验时间和经费,而且提高了染色效果,能更准确地反映心肌缺血再灌注损伤的程度。因此改进后的心肌TTC染色法是一种经济、简便、快捷、高效的染色方法。
Objective To evaluate an improved modification of TTC staining method for measuring myocardial in-farct size after ischemia-reperfusion in rats.Methods Twenty healthy SPF male 8-week-old SD rats were randomly divided into two groups:Group A with conventional TTC staining, and group B with the modified TTC staining method for measuring myocardial ischemia-reperfusion injury.The infarct size was caculated and the serum cTnI levels were determined.Results The infarcted myocardium was well detected in both groups A and B.There were no significant differences in the myocardial infarct sizes measured in the groups A and B (48.69 ±5.37 %vs.47.41 ±3.28%, P>0.05).There were no significant differences in the serum cTnI levels assayed in the groups A and B (4.51 ±0.88 ng/mL vs.4.70 ±0.71 ng/mL, P>0.05). But compared with the group A, the color contrast of stained myocardial slice and the distinguishing infarction area and non-infarction area were much clearer in the group B.Conclusi

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以灰木莲( Manglietia glauca)的新鲜花粉为材料，研究不同浓度蔗糖、硼酸和氯化钙组合的培养基对灰木莲花粉离体萌发的影响，并用TTC染色法、 I-IK染色法和无机酸法对灰木莲的花粉生活力进行了测定。结果表明：在pH值6．0时，以150 g/L蔗糖+50 mg/L硼酸+100 mg/L氯化钙+7 g/L琼脂的培养基配方最适合灰木莲花粉的培养，离体萌发率达到62．2%，花粉管生长良好；无机酸法不适宜灰木莲花粉生活力的检测；TTC染色法和I-IK染色法的测定结果与离体萌发法的结果较接近，分别为60．6%和64．1%，但I-IK染色法在观察判断上较为困难。因此， TTC染色法是快速检测灰木莲花粉生活力的最佳方法。
The fresh pollen of Manglietia glauca was used as material to study the effect of mediums with different concentrations of sucrose, boric acid and calcium chloride on in vitro pollen germination . And the pollen viabilities of M. glauca were tested by using 2 , 3 , 5-triphenyltetrazolium chloride staining ( TTC) , iodine-potassium iodide staining ( I-IK) and mineral acid test. The results showed that when the pH was 6. 0, the optimum medium consisting of 150 g/L sucrose, 50 mg/L boric acid, 100 mg/L calci-um chloride and 7 g/L agar was suitable for M. glauca pollen cultured. The in vitro germination rate was 62. 2% and the pollen tube grew well. The mineral acid test was unsuitable for detecting the pollen via-bility. The results tested by TTC and I-IK methods were similar to that of in vitro germination method with pollen viabilities 60. 6% and 64. 1% respectively. However, it was difficult to distinguish the stained pollen grains using the I-IK method. The TTC method was th

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为了探索菱花粉生活力的最佳测定方法,以孝感红菱花粉为试材,利用无机盐培养法、MTT(四甲基噻唑蓝)染色法和TTC(氯化三苯基四氮唑)染色法对花粉生活力进行测定。研究结果表明,培养基中添加低浓度的蔗糖和硼酸对菱花粉的萌发有促进作用,菱花粉萌发的最适培养基为10%蔗糖+0.005%硼酸,萌发率达65.9%;用MTT染色法和TTC染色法测定菱花粉生活力,染色率仅为36.9%和27.1%,效果不理想。
In order to explore suitable methods for pollen viability determination of water chestnut, the pollen viability of cultivar Xiaoganhongling was determined by using the methods of inorganic salt culture, MTT staining and TTC staining. The results showed that the medium supplemented with low concentrations of sucrose and boric acid could promote the germination of pollen, and the optimum medium for pollen germination was 10% sucrose+0.005% boric acid, with the germination rate of 65.9%. The MTT staining and TTC staining methods were not ideal for pollen viability determination of water chestnut, because their dyeing rates were merely 36.9%and 27.1%.

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采用蔗糖萌发、醋酸洋红染色、TTC染色、I2-KI染色、红墨水染色法，分别测定了6个椰子（CocosnucifemL．）品种的花粉活力，并比较了各方法的优、缺点。试验结果表明：前4种方法的测定效果较好；在6个品种中，杂交种文椰78F。的花粉活力最高，海南高种的花粉活力较高。
The pollen viability of six coconut ( Cocos nucifera L.) varieties was determined by using five methods ( sucrose-based germination, aceto-carmine dyeing, TTC dyeing, I2 -KI dyeing, and red ink dyeing) respectively, and the advantages and disadvantages of these five methods were compared .The test results showed that the former four methods were better than the last one.Among six varieties, hybrid Wenye 78F1 had the maximum pollen viability , and the pollen viability of Hainan Tall was higher than that of other four varieties .

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为藜豆的杂交育种及传粉生物学研究等提供基础资料与科学依据,筛选适宜的花粉活力快速测定方法,以白花黑籽藜豆花粉为材料,用光学显微镜观察花粉形态及萌发花粉管的数目,并用TTC法、IKI法和醋酸洋红法对其花粉活力进行了测定。结果表明:成熟的藜豆花粉近似圆球形,有3个萌发孔,但只有1个萌发孔长出花粉管;TTC法着色效果差,I-IK着色时间长(约20min),而醋酸洋红染色法着色快(约2min),染色清晰;醋酸洋红法适合藜豆花粉活力的快速测定。
In order to provide basic data and scientific evidence for the study of hybrid breeding and pollination biology,and also to select the suitable method for rapid measurement of life viability,the study was carried out to observe the pollen morphology and number of germination pollen tube of S.cochinchinesis by optical microscope,to observe the pollen morphology using TTC staining method,I-KI staining method and aceto-carmine staining method and to measure the life viability of the velvet bean pollen.The results indicated that the mature pollen was of approximately round ball form and had three germination apertures,but only one of which would grow up with pollen tube.The I-KI staining method and the TTC staining were the inappropriate methods for bad staining method and long staining time(about 20 min)respectively.The method of aceto-carmine staining was the best way to determine the pollen viability of S.cochinchinesis for staining rapidly and ideal staining effect.

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目的 研究大鼠局灶性脑缺血再灌注MRI弥散加权成像(DWI)的变化,探讨其与TIC染色变化的关系. 方法 44只雄性SD大鼠按随机数字表法分为假手术组和脑缺血2h、脑缺血6h再灌注不同时间组(0 h、0.5h、2h、6h、24 h),每组各4只.脑缺血2h组、脑缺血6h组采用线栓法制作成大脑中动脉栓塞模型.各组大鼠于不同再灌注时间点行MRI DWI扫描,结束后立即处死,取相应部位脑组织行TTC染色,观察分析其与DWI成像的关系. 结果 脑缺血2h组、脑缺血6h组再灌注0h均可见部分右侧大脑半球DWI呈高信号,表观扩散系数(ADC)值降低,TTC染色可见部分右侧大脑半球失染呈白色.缺血2h组再灌注24 h后DWI异常信号区相对面积明显变小,ADC值增大,TTC失染相对面积变小,较再灌注0h差异有统计学意义(P＜0.05),而脑缺血6h组则差异无统计学意义(P＞0.05).脑缺血6h组再灌注不同时间点较脑缺血2h组再灌注相应时间点DWI异常信号区相对面积大,ADC值低,TTC失染相对面积大,差异有统计学意义(P＜0.05).脑缺血2h组、脑缺血6h组再灌注相对应时间点DWI异常信号区面积与TTC染色失染区面积比较差异无统计学意义(P＞0.05). 结论 脑缺血再灌注后可用MRI DWI成像评价脑缺血面积.
Objective To investigate the changes of MR diffusion imaging (DWI) appearance in rats after focal cerebral ischemia reperfusion,and explore its relation with the changes of triphenyl tetrazolium chloride (TTC) staining.Methods Forty-four male Sprague Dawley rats were randomly divided into a sham-operated group (n=4) and two experimental groups (n=20,ischemia for 2 and 6 h,respectively); rats in the experimental groups were performed occlusion of the right middle cerebral artery (MCAO) with thread,and reperfusion for 0,0.5,2,6 and 24 h (n=4).DWI was performed at each time point; and then,the rats were sacrificed and TTC staining was performed.Results Before reperfusion,DWI demonstrated hyperintensity in the right hemisphere in the experimental groups,the ADC value was decreased,and white area was noted by TTC staining.The experimental group of ischemia for 6 h had larger area of DWI abnormal signal and decreased apparent diffusion cofficient (ADC) value as compared with those in the exp

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为研究荷花花粉的生活力,选择了20个荷花品种分别用TTC染色法、亚甲基蓝染色法和离体萌发法测定了花粉的染色率和萌发率,并研究了不同温度贮藏条件对花粉生活力的影响.结果表明,亚甲基蓝染色法不能作为荷花花粉生活力的测定方法,离体萌发法结果更直观和准确;20个荷花品种间的离体萌发率差异明显,最高为13.1%,最低为0;不同品种在三种贮藏温度下萌发率变化趋势相同,4℃条件下贮藏较有利于荷花花粉生活力的保持.
To investigate the pollen viability of lotus,the pollen dyeing rate and germination rate of 20 lotus cultivars were determined by three testing methods including TTC coloration,methylene blue staining and culture in vitro. The effect of different storage temperature on the pollen vitality was also researched. The results indicated that the methylene blue staining is not suitable to be used for testing the lotus pollen viability and the results of culture in vitro are more accurate and visual than the others. There were significant differences in germination rate among the 20 lotus cultivars,the highest germination rate was 13.1% and the lowest was 0. The changing trends of germination rate were same under the three storage temperature among the different cultivars and the most proper temperature was 4℃for lotus pollen storage.

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以新鲜桑葚浆果作为酵母菌的分离源，从桑葚自然发酵过程中分离酵母菌，进而对所选菌株进行两级筛选。经过初步分离共得到了50株酵母，进一步TTC染色分离，共得到可产酒精的酵母28株，其中发酵前期14株，发酵中期8株，发酵后期6株。
In this study, yeasts were isolated from mulberry fruits by means of natural fermenta-tion. The appropriate mulberry wine yeasts were then screened in two steps. 50 yeast strains were isolated from preliminary screening, and 28 yeast strains, which can produce alcohol, were isolated by means of TTC. In these yeasts, 14 strains came from prophase fermentation, 8 strains from metaphase fermentation and 6 strains from anaphase fermentation.

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目的：通过改良线栓法建立稳定的Wistar大鼠大脑中动脉脑缺血/再灌注模型。方法：60只雄性Wistar大鼠，参考Longa法并适当改进建立大鼠大脑中动脉脑缺血/再灌注模型30只，对照组30只。实验组结扎右侧颈总动脉近心端，夹闭颈总动脉远心端，颈总动脉剪一小口，从颈总动脉经颈内动脉插入线栓，缺血1 h后，在麻醉状态下完全拔出线栓，并结扎颈内动脉远心端。对照组结扎右侧颈内动脉近心端及远心端，但不插入线栓。术后取脑组织行TTC染色，并进行神经功能评定。结果：经改良线栓法制作脑缺血再灌注损伤模型，成功率达82.75%，平均操作时间约（20.4±5.3）min，模型大鼠右侧眼裂变小，行走时向右侧转圈，模型大鼠神经功能评分均在3~4分，经TTC染色梗死侧大脑半球颜色苍白，脑梗死区范围一致，梗死体积约（34.62±5.32）%；对照组无任何临床症状，无神经功能缺失表现，经TTC染色无梗死灶。结论：通过改良，成功建立了Wistar大鼠大脑中动脉脑缺血/再灌注模型。
Objective:To establish a simple, reliable model of middle cerebral artery occlusion-reperfusion model in wistar rats.Method:On the basis of the method of Longa, 60 male wistar rats were randomly divided into two groups, 30 rats in each group. In the experimental group, the proximal part of the right common carotid artery (CCA) was ligated, the distal part of the CCA was clipped, then a small cut was made in CCA, thread was put into the internal carotid artery (ICA) through the cut of CCA to block the blood flow in the right of middle cerebral artery. One hour later, the thread was draw out under the condition of anesthetization, then the distal part of right CCA was ligated. In the control group, the proximal and distal part of the right common carotid artery (CCA) was ligated without putting thread into ICA. After operation, brain tissue was taken out to undergo TTC staining, and neurological deficit sign scores were performed. Result:The successful rate of modified MCAO/R m

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目的观察脑梗死大鼠经人脐带间充质干细胞(umbilical cord-derived mesenchymal stem cells,UC-MSCs)治疗后,行为学的恢复情况,2,3,5—氯化三苯基四氮唑(triphenyl tetrazolium chloride,TTC)染色以及胆碱能的变化。方法制备脑梗死大鼠大脑中动脉闭塞(Middle cerebral artery occlusion,MCAO)模型,将UC-MSCs注入脑梗死大鼠体内,对大鼠运动功能进行评分,比较TTC染色显示的脑梗死区域所占比例,测定脑组织和血浆中乙酰胆碱酯酶(True choline esterase,TChE)活性和乙酰胆碱(Acetylcholine,Ach)浓度。结果与模型组相比,UC-MSCs组大鼠行为学恢复明显(P0.05),UC-MSCs组大鼠脑组织和血浆乙酰胆碱酯酶(TChE)和乙酰胆碱(Ach)活性显著增强(P0.05)。结论 UC-MSCs经尾静脉移植可促进脑梗死后神经功能恢复,增强大鼠胆碱能系统活性。
Objective To observe the effects of intravenous administration of human umbilical cord-derived mesen-chymal stem cells(UC-MSCs)after middle cerebral artery occlusion(MCAO)in adult rats.Methods Adult male SD rats were administrated by MCAO.Functional outcome measurements using Beam walking Test(BWT)were performed at 24 hour of MCAO group and 10 days of UC-MSCs treatment group respectively.TTC staining ,TChE and Ach activity array were also used.Results There was significant neurological function improvement in rats treated with MSC com-paring with that of control groups(P <0.05),and also Ach and TChE activity (P <0.05).Conclusion Intravenous ad-ministration of UC-MSCs promotes the neurological function in rat MCAO model.UC-MSCs play an advantageous function on cholinergic neurotransmitters in the case of lesion.

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