酱鸭加工过程(盐腌、酱制、收汁、灭菌)中质构特性(硬度、弹性)和肌肉蛋白(肌浆蛋白、肌原纤维蛋白)降解的变化规律。结果表明:在盐腌过程中,鸭肉硬度呈现先升高后降低趋势,盐腌6h硬度最高,12h硬度最低;盐腌过程中的弹性总体呈下降趋势,腌制9h后弹性最低。酱制、收汁、灭菌过程鸭肉硬度明显高于盐腌过程,在酱制阶段,硬度变化不显著,经过灭菌后的酱鸭硬度有所下降;该过程中的鸭肉弹性低于盐腌过程,变化不显著。SDSPAGE电泳分析显示:在盐腌阶段,鸭肉肌原纤维蛋白中的α-肌动蛋白素条带比较稳定,其余的肌原纤维蛋白和肌浆蛋白均发生着不同程度的降解;而经过酱制、收汁、灭菌后,肌原纤维蛋白条带变少,肌浆蛋白中原先的蛋白条带消失,出现了一些新的蛋白条带。从盐腌开始到酱制结束,非蛋白氮含量下降,灭菌后含量增加;鸭肉重量在加工过程中下降,特别是经过0.5h的酱制后,鸭肉重量显著降低。
The changes of texture (hardness and elasticity)and degradation of muscle proteins (sarcoplas-mic and myofibrillar proteins)during processes (salting,saucing,juice absorbing and sterilization)of sauced duck were studied.During the salting process,the hardness of duck meat increased firstly then de-creased with the highest hardness achieved at 6h and the lowest one at 12 h.Generally,the elasticity de-creased during the salting process,down to the lowest level at 9 h.The hardness at saucing process,j uice absorbing and sterilization were higher than that at salting process.The hardness did not change obviously during the saucing process,but it decreased after sterilizing.The elasticity at saucing,j uice absorbing and sterilization were lower than that at salting process with no significant change SDS-PAGE results revealed thatα-actinin was stable while other myofibrillar proteins and sarcoplasmic proteins were degraded during the salting process.After saucing,j uice absorbing