观察电针足三里穴对失血性休克大鼠肠胶质细胞形态的影响,探讨电针足三里穴治疗胃肠疾病的机制。方法:将18只SD雄性大鼠随机分为假手术组、模型组和电针组,每组6只。按总血容量的45%放血制备失血性休克模型。电针组于制模完成后10 min电针足三里穴(持续刺激1.5 h,2~100 Hz,2 mA);模型组同样方法电针非经非穴;假手术组只分离股动、静脉不放血。失血后6 h后处死大鼠,取肠道组织制作肠肌间神经丛全层铺片,采用免疫荧光法进行肠胶质纤维酸性蛋白(GFAP)染色,荧光显微镜下观察肠胶质细胞形态,同时对肠道GFAP蛋白含量进行WesternBlot测定。结果:失血后6 h,肠胶质细胞出现形态学异常,细胞变形并且荧光增强,突触模糊、中断;电针足三里穴能减轻肠胶质细胞形态学异常,增加荧光强度。与假手术组相比,模型组GFAP表达量增加,但电针足三里穴后GFAP的表达量增加更为显著(P0.05)。结论:大鼠失血性休克后电针足三里穴能激活并保护肠胶质细胞。
Objective:To investigate the effects of electro-acupuncture of Zusanli point (足三里穴)on enteric glial cells of the rats after hemorrhagic shock,and inquire into the mechanism of electro-acupuncture of Zusanli points in treating gastrointestinal disease. Methods:Eighteen male SD rats were randomly divided into three groups (n=6):sham operation group,model group,and electro-acupuncture group. Hemorrhagic shock was replicated by withdrawing 45% of the calculated total blood volume. Rats in electro-acupuncture group received electro-acu-puncture at Zusanli points (ST36)10 minutes after blood loss (electro-acupunctured for 1.5 hours,2-100 Hz, 2 mA). Electro-acupuncture at non-channel acupoints was performed in rats of model group. Rats in sham opera-tion group did not suffer from blood loss. Six hours after hemorrhage,animals were sacrificed,and distal small in-testine was harvested to prepare whole mount slide of myenteric plexuses. Morphological changes in enteric glial cells