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双语推荐：广西巴马

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目的克隆广西巴马小型猪PGC-1α基因编码区（CDS）序列,利用RT-PCR和QRT-PCR方法分析PGC-1αmRNA组织表达情况。方法本实验以广西巴马小型猪背最长肌cDNA为模版,PCR扩增PGC-1α基因CDS序列,将其连接至pEASY-T5载体,转染细菌、验证和序列测定;通过RT-PCR半定量和QRT-PCR实时荧光定量检测PGC-1α基因在小型猪多个组织中的表达情况。结果克隆获得广西巴马小型猪PGC-1α基因CDS序列,全长2391 bp,编码796个氨基酸,与参考序列的同源性为99.9%,两处碱基发生同义突变,分别是C-A1105和GA1524;PGC-1α基因在广西巴马小型猪心脏和肾脏中的表达丰度最高,其次是肝脏、皮下脂肪和背最长肌,而在胰腺中未检测到其表达。结论成功克隆了广西巴马小型猪PGC-1α基因编码区序列并进行了多种组织表达分析,为后续研究PGC-1α在小型猪2型糖尿病发生过程中作用途径打下基础。
Objective To clone the coding sequence of Guangxi Bama mini-pig PGC-1αgene, and to analyze the expression of PGC-1αgene in various tissues of mini-pigs using RT-PCR and QRT-PCR techniques.Methods The PGC-1αgene coding sequence ( CDS) was amplified by PCR from the cDNA of longissimus muscle of Guangxi Bama mini-pig. The PCR products were inserted into pEASY-T5 vector, transfected E.coli, identified and sequenced.The PGC-1αgene expression in different tissues of the Bama mini-pigs was detected by RT-PCR and QRT-PCR assays.Results The PGC-1αgene CDS of Guangxi Bama mini-pig was cloned.It was 2391 bp in length.It had 99.9%homology with the reference sequence, and had two synonymous mutations that were C-A1105 and G-A1524.The expression level of PGC-1αgene was higher in the heart and kidney, followed by liver, subcutaneous fat and longissimus muscle, but the expression was not de-tected in pancreas of Guangxi Bama mini-pig.Conclusions We have successfully cloned the PGC-1αg

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采用ISSR分子标记对陆川猪及其肉制品进行鉴伪识别，利用ISSR技术，对纯种陆川猪、陆川猪杂交品种（二元杂猪和三元杂猪）及广西本地的巴马香猪和环江香猪的DNA指纹图谱进行分析。结果表明：从34条引物中筛选出3条带型清晰、重复性好的引物WM12、WM27、WM10，其中引物WM12可用于鉴别陆川猪、二元杂猪和三元杂猪的生鲜样品及腊肉制品，引物WM27可用于鉴别陆川猪和巴马香猪的生鲜样品及腊肉制品，引物WM10用可于鉴别陆川猪和环江香猪的生鲜样品及腊肉制品。ISSR技术可快速准确的鉴定陆川猪、陆川猪杂交品种、巴马香猪、环江香猪及其肉制品。
In this study, species identification of Luchuan pig meat and meat products was carried out by DNA fingerprint profiling of meats from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs using ISSR (inter-simple sequence repeat) molecular markers. The results showed that 3 clear and repeatable primer pairs WM12, WM27 and WM10 were selected from 34 primers. WM12 was useful for discriminating fresh and cured meat from purebred Luchuan and hybrid Luchuan pigs, while Luchuan pigs could be distinguished from Bama miniature pigs by using WM27, and from Huangjiang miniature pigs by using WM10. In conclusion, the ISSR method enables rapid and accurate identification of fresh meat and meat products from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs.

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广西河池市巴马县定金寺瓦氏夫人庙是瓦氏夫人崇拜的外在表征，庙宇本身的变化历程及土司夫人的身份认同，都表现出瓦氏夫人崇拜与岑大将军崇拜互为“独立”与“依附”的循环共存。瓦氏夫人崇拜的“扩散”，也是基于信众的现实需要。
The Mrs.Vachelli Temple of Bama Dingjin Temple is the external representation of the worship of Mrs.Vachelli.Its change and the identification of chieftain''s wife,have shown the worship of both Mrs. Vachelli and General Cen,which is also based on the believers''practical needs.

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在城乡统筹发展、养生旅游热潮兴起的背景下，旅游产业成为新农村建设和农村经济结构转型的重要切入点。本文对旅游型村庄的概念、分类和发展模式进行了界定，并以巴马坡月村为例，针对广西旅游型村庄现状存在的突出问题，提出具体的发展策略和优化整治措施。
Together with status, this paper puts forward development strategies and optimization measures for planning of tourism-type villages in Guangxi.

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目的：探讨广西巴马长寿人群血清胰岛素样生长因子Ⅰ（IGF -Ⅰ）水平及其影响因素。方法2008年5月采用随机抽样的方法在广西巴马长寿区抽取160名长寿老人（90~110岁）作为长寿组，另抽取一般地区健康青壮年人（20~50岁）102名作为青壮年对照组。测量其身高、体质量、腰围、血压、血糖、血脂等，应用酶联免疫吸附法检测血清IGF -Ⅰ水平，利用聚合酶链反应-限制性片段长度多态性（ PCR - RFLP）法分析长寿老人胰岛素样生长因子Ⅰ受体（IGF -ⅠR）基因3174位点多态性，并对长寿老人血清IGF -Ⅰ水平的影响因素进行多元逐步回归分析。结果长寿组血清IGF -Ⅰ水平低于青壮年对照组〔（114,0±31,5）μg/ L 与（140,7±37,3）μg/ L，F =6,321，P<0,05〕。长寿组中，GG、GA 和 AA 基因型携带者血清IGF -Ⅰ水平分别为（115,3±30,4）μg/ L、（112,1±34,5）μg/ L 及（113,2±27,3）μg/ L，差异无统计学意义（F =1,030，P =0,386）。多元线性回归分析显示，血清IGF -Ⅰ与体质指数和高密度脂蛋白胆固醇（HDL - C）呈正相关，与性别、年龄、民族、臀围、腰围、血压、血糖、总胆固醇、三酰甘油和低密度脂蛋白胆固醇均无直线相关关系。结论广西巴马长寿人群血清IGF -Ⅰ水平明显低
Objective To investigate the serum lerels of insulin - like growth factor - Ⅰ(IGF - Ⅰ)and tis influence factors in the long - lived elderly in Bama county of Guangxi, Methods 160 healthy long - lived elderly aged from 90 to 110 years were randomly selected as long - lived elderly group from Bama county longevity area in Guangxi in May 2008, 102 healthy young adults aged from 20 to 50 years were selected as control group from general district in Guangxi, Height,weight,waist circ-umference,blood pressure,fasting blood glucose and serum lipid were measured, ELISA method was applied to determined serum IGF - Ⅰ levels,and insulin - like growth factor Ⅰ receptor(IGF - ⅠR)gene 3174 polymorphism were genotyped with PCR -RFLP, The possible influencing factors of the levels of IGF - Ⅰ the long - lived elderly were analyzed by multiple linear regression models, Results The levels of serum IGF - Ⅰ in long - lived elderly group were significantly lower than those in control group〔

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建设生态文明是我国经济社会可持续发展的必然要求,对于我国现实和未来的发展意义重大.经济发展水平较低、生态环境脆弱的西部少数民族地区,生态文明建设的程度与效应更加关乎当地经济的永续发展,关系到国家边疆的稳定团结.本文以广西巴马瑶族自治县生态文明建设实践为个案研究,在介绍该县生态文明建设现状的基础上,对其生态文明建设存在的问题及原因进行分析,最后探讨了推进建设生态文明的建议与措施.
It's necessary to construct ecological civilization for sustainable econom ic and social developm ent inChina,anditisofgreatsignificancefornationalrealityandfuturedevelopment.W ithlowerlevelsofeconomic developm ent and fragile ecological environm ent, the extent and effect of ecological civilization construction in W estern m inority areas w illinfluence m ore in sustainable developm entofthe localeconom y and the stability unity of national frontiers. B am a Y ao autonom ous county in G uangxi w as taken as a case, this paper described the pre-sent situation ecological civilization construction in B am a, and then analyzed its problem s and reasons. Finally, proposals thathow to prom ote the construction ofecologicalcivilization w ere putforw ard.

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目的探讨DNA甲基化与巴马长寿的关系。方法在广西巴马县长寿家族中选取60例90~110岁老人组成长寿组,在非长寿家族中选取48~85岁的健康者60例组成对照组。抽取外周血2 ml,构建DNA混合池,利用Roche-Nimble Gen甲基化芯片技术对研究人群的全基因组进行扫描,使用SPSS17.0软件及在线分子功能注释系统(MAS)进行数据分析,P0.01视为甲基化程度较高的序列。结果共筛查出6 441个超甲基化位点,其中长寿组392个,对照组6 049个。高甲基化位点共涉及1 618个基因,长寿组有133个,对照组有1 485个。两组人群的高甲基化基因均涉及24个生物学功能,均以细胞过程、生理过程、生物调控为主。长寿组高甲基化基因涉及42个信号通路,对照组涉及156个信号通路。长寿组和对照组中均有与年龄相关性疾病有关联的高甲基化基因,长寿组中涉及较多疾病的基因有CCKBR、COL18A1、EGR3、H19、IL11、TIMP1、S100A1等;对照组中涉及较多疾病的基因有ACSL1、ADAMTS13、ADCY8、AHRR、ARVCF、ATP2A1、ATP5I、BCS1L、GCK、ITGA2B等。结论本研究利用芯片技术筛查获得了与长寿呈正相关和负相关的高甲基化位点、基因及其生物学功能分类、信号通路,为DNA甲基化和长寿的深入研究提供了基础数据。
Objective To seek for hypermethylation sites and genes ,and explore the correlationship between DNA methylation and longevity in Guangxi Bama .Methods Sixty aged people with long-live family history from 90 to 11o years old were recruited into longevity group ,and another sixty individuals without long-live family history aged from 48 to 85 were recruited into control group 2. ml peripheral blood was extracted to construct the DNA gene pools ,then the whole genome was scanned by the Roche NimbleGen methylation array technology , and the final data was analyzed by SPSS 17.0 software and the online molecular functional annotation system ,it was significant difference when P<0.01.Results 6441hypermethyaltionsiteswerescreenedout,392sitesforthelongevitygroupand6049sitesforthecontrolgroup.The hypermethylation sites involved in 1 618 genes,133 hypermethylation genes for the longevity group and 1 485 ones for the control group .The hypermethylation genes from both groups involved in

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背景：用自体骨皮瓣移植是目前修复复合组织缺损的最好方法，但其修复能力有限、创伤大、造成新的组织缺损和一定功能障碍。异体骨具有骨诱导能力，可能用来预制骨皮瓣。目的：观察异体骨异位埋植诱导预制骨皮瓣过程中异体骨的病理变化。方法：实验动物为广西巴马微型猪，将深低温冷冻异体骨埋植于旋髂浅动脉供养的皮瓣组织内，植入后在体观察局部反应情况，于植入后4，8，12，16周随机取出异体骨，行大体观察及病理切片镜下观察。结果与结论：所有异体骨植入处无明显炎症反应。病理检查可见异体骨在皮瓣组织内埋植后4周异体骨表层出现血管化，未见明显类骨母细胞；植入后8周出现异体骨不同程度的血管化、骨吸收和分布不均的类骨母细胞和类破骨细胞，有新骨形成；植入后12周新骨形成进一步增加，但骨吸收亦加重，植入骨形态改变；植入后16周异体骨碎裂吸收，新骨形成无明显增加。结果提示异体骨异位埋植预制骨皮瓣过程中，异体骨随时间不断发生病理变化，需适时移植。
BACKGROUND:Autologous bone-skin flap transplantation is the best method for the repair of composite tissue defect, but the repair ability is limited, with big trauma, new tissue defect and a certain dysfunction. Al ogeneic bone has the osteoinductive capacity, which can be used to prepare the bone-skin flap. OBJECTIVE:To research the pathological change of the al ogenic bone during the prefabrication of bone-skin flap with al ogeneic bone implant. METHODS:The experimental animals were Bama miniature pigs. Deep-frozen al ogenic bone was implanted in iliac artery-supported tissue flap compartment of miniature pigs. After operation, the local reactions were observed, the al ogenic bone were studied by general observation and histological analysis at 4, 8, 12 and 16 weeks after implantation respectively. RESULTS AND CONCLUSION:Obvious inflammation reaction was not observed in the surgical zone. The al ogeneic bone was vascularized at 4 weeks after implanted into the flap tissue without obv

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目的研究经彩色多普勒超声定位体外超声聚焦刀在去肾交感神经术中的安全性和有效性。方法 8个月大健康雄性广西巴马小香猪18只,随机分为三组,消融组1、消融组2及对照组,每组6只。消融组1行超声聚焦刀双侧肾动脉交感神经消融术,每一点单次消融强度为280 W×2 s;消融组2每点消融强度为320 W×2 s;对照组于麻醉后仅以CDFI行肾动脉超声检查。所有动物于术前、术后即刻、术后7 d和28 d测血压、采集血样,并评估血液指标(包括去甲肾上腺素、肾功能等),术后28 d处死并观察组织形态与病理变化。结果与手术前比较,消融组2手术后第7 d收缩压下降[(132.67±6.86)mm Hg vs.(122.33±6.15)mm Hg],差异具有统计学意义(P0.05),第28 d收缩压下降[(132.67±6.86)mm Hg vs.(117.83±6.49)mm Hg],差异具有显著统计学意义(P0.01)。与对照组比较,消融组2在术后第7、28 d收缩压下降,差异具有统计学意义(P均0.05)。与手术前比较,消融组1、消融组2术后7d、术后28 d测得血浆NE浓度降低,差异具有显著统计学意义(P均0.01)。与对照组和消融组1比较,消融组2术后7 d与术后28 d NE浓度降低,差异具有显著统计学意义(P均0.01)。三组术前与术后、三组之间血肌酐、尿素氮、尿酸比较,差异无统计学意义(P均0.
Objective To study the efficacy and safety of extracorporeal high-intensity focused ultrasound (HIFU) through color Doppler ultrasonic locating in renal sympathetic denervation.Methods Male Bama minipigs (aged 8 months old,n=18) were randomly divided into ablation group 1 (HIFU=280 W×2 s), ablation group 2 (320 W×2 s) and control group (only CDFI, eachn=6). The blood pressure was detected, blood samples were collected and blood indexes [including norepinephrine (NE) and renal function] were reviewed in all minipigs before operation, immediately after operation, and 7 d and 28 d after operation. After operation for 28 d the minipigs were euthanatized and the changes of histopathology were observed.Results After operation for 7 d, systolic blood pressure (SBP) decreased [(132.67±6.86) mmHgvs. (122.33±6.15) mmHg,P 0.05) in 3 groups before and after operation and among 3 groups. The observation of light microscope showed that vascular wall membrane structure was completed wit

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目的 探讨盐酸戊乙奎醚对体外循环(CPB)后肝脏损害的保护作用及机制.方法 选取32 ～ 40 kg健康的广西巴马小型猪,24头猪随机分为3组(n=8):假手术组(Sham组)、模型组(Mod组)和盐酸戊乙奎醚组(PHC组).Sham组只插管不行CPB,Mod组行CPB静脉注射生理盐水,PHC组CPB前10 min静脉注射盐酸戊乙奎醚0.1 mg/kg.3组分别于CPB前(T0)、CPB后1h(T1)、CPB结束时(T2)和术毕(T3)抽静脉血检测血清中天冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)活性和直接胆红素(DB)、总胆红素(TB)含量.3组于CPB前和CPB结束时,分别取约50μg的肝组织制成10％的组织匀浆,检测肝组织匀浆的三磷酸腺苷(ATP)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性并在电镜下观察肝组织的超微病理改变.结果 与Sham组比较,Mod组和PHC组CPB结束时各时点AST、ALT活性和DB、TB和MDA含量升高,ATP含量和SOD活性下降(P＜0.05);与Mod组比较,PHC组CPB结束时的AST、ALTDB、TB和MD下降,ATP、SOD升高(P＜0.05),电镜下的肝组织病理损伤减轻.结论 盐酸戊乙奎醚对CPB后肝脏损害有一定的保护作用,其机制可能与抗氧化清除氧自由基有关.
Objective To investigate the effects of penehyclidine hydrochloride on liver injury induced by cardiopulmonary bypass (CPB) in Guangxi bamaminiature pig.Methods Twenty-seven Bama miniature pigs weighted 32-40 kg were chosen,three pigs were used for sampling blood,another twentyfour pigs were used for cardiopulmonary bypass (CPB).Twenty-four pigs were randomly divided into three groups (n=8 each):group Sham,group Mod,and group PHC.Propofol,fentanyl and vecuronium were continued by intravenous injection and inhaled isoflurane interruptly for anesthesic maintain.CPB was performed on mild hypothermic beating heart for 120 min.Pnehyclidine hydrochloride 0.1 mg/kg was injected intravenously before 10 min CPB.Blood samples were collected for aspartate aminotransferase (AST),alanine transaminase (ALT),direct bilirubin (DB) and total bilirubin (TB) before CPB (T0),after CPB 1 h (T1),CPB cease (T2) and operation cease (T3).The content of ATP,MDA and SOD in liver tissue at befor and aft

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