检测盐酸小檗碱和苦参碱联合氨苄西林对表皮葡萄球菌的抗菌作用.利用二倍稀释法、微量棋盘稀释法、时间-杀菌曲线评估法对8株临床分离的表皮葡萄球菌进行药物敏感性测定.结果表明,盐酸小檗碱、苦参碱、氨苄西林单药对表皮葡萄球菌的最小抑菌质量浓度分别为0.8~3.1μg/mL、12.5mg/mL和0.5~16.0μg/mL.盐酸小檗碱联合氨苄西林作用于8株分离株后,盐酸小檗碱和氨苄西林的最小抑菌质量浓度分别下降了88%~94%和75%,抑菌浓度指数为0.187~0.498;苦参碱联合氨苄西林作用于8株分离株后,苦参碱和氨苄西林的最小抑菌质量浓度分别下降了80%和75%~94%,抑菌质量浓度指数为0.188~0.450.2种生物碱联合氨苄西林作用于表皮葡萄球菌的时间-杀菌曲线显示,菌落减少量大于等于2lg N.盐酸小檗碱、苦参碱与氨苄西林联合作用表皮葡萄球菌均具有抗菌协同作用.

This article is about testing the antibiotic action of berberine hydrochloride and matrine ’s combining with ampicilin on staphylococcus epidermidis.8 clinical isolated staphylococcus epidermidis were tested for drug susceptibility with two-fold dilution method,micro-chessboard assay method and time-sterilization curve evaluation method.The result shows that the minimum bacteriostasis concentration of berberine hydrochloride, sophocarpidine and ampicillin to staphylococcus epidermidis were 0.8-3.1μg/mL,12.5 mg/mL and 0.5-16.0 μg/mL respectively. After the action of berberine hydrochloride’s combining with ampicillin on 8 isolated staphylococcus, the minimum bacteriostasis concentration of berberine hydrochloride and ampicillin were down,88%-94% and 75% respectively,and fractional inhibitory concentration index was 0.187-0.498;The minimum bacteriostasis concentration of sophocarpidine and ampicillin declined,80% and 75%-94% respectively after the action of sophocarpidine c

目的了解儿童呼吸道流感嗜血杆菌的临床分布特征和耐药特点,为指导临床合理用药提供科学依据。方法收集儿童痰液标本进行培养并分离出流感嗜血杆菌,用K-B纸片扩散法进行抗菌药物敏感试验,并对其进行β内酰胺酶测定;数据用WHONET5.6统计软件统计分析。结果从12 374份痰液标本中共分离出1 256株流感嗜血杆菌,分离率为10.2%;1 256株流感嗜血杆菌对氨苄西林、甲氧苄啶-磺胺甲口恶唑和氨苄西林-舒巴坦的耐药率分别为37.8%、65.5%和16.5%,对其余测试的抗菌药物耐药率均10.0%;β内酰胺酶阳性率为33.5%。结论流感嗜血杆菌对氨苄西林和甲氧苄啶-磺胺甲口恶唑的耐药性较高;该菌对于头孢克洛、氨苄西林-舒巴坦、头孢他啶、头孢克肟、阿奇霉素、环丙沙星、美罗培南、利福平的敏感率均在80.0%以上。产β内酰胺酶是流感嗜血杆菌对氨苄西林的主要耐药机制。

Objective To investigate the antibiotic resistance of Haemophilus influenzae isolates collected from the children with respiratory tract infection for rational use of antibiotics in clinical practice .Methods The H .influenzae strains were isolated from children and subjected to antimicrobial susceptibility testing by Kirby-Bauer method .Nitrocefin disc test was used to detect the production of beta-lactamases .WHONET 5 .6 software was used to analyze the susceptibility data .Results A totalof1256strainsof H.influenzaewereisolated.About37.8% ,65.5% and16.5% ofthe1256strainsof H.influenzae were resistant to ampicillin ,trimethoprim-sulfamethoxazole ,and ampicillin-sulbactam ,respectively .Less than 10 .0% of these strains were resistant to any other antibiotics tested .Beta-lactamase was produced in 33 .5% of the 1 256 strains of H . influenzae .Conclusions The H . influenzae strains in this study are mainly resistant ampicillin and trimethoprim-sulfamethoxazole .About 80 .0%

目的：制备含 pCMV-IL-2-IRES-NK4质粒减毒沙门菌 TPIN 并检测其稳定性。方法将 pCMV-IL-2-IRES-NK4质粒转进减毒沙门菌 Ty21a 感受态,制备成重组的减毒沙门菌菌株 TPIN；将 TPIN 在含有氨苄西林(+)和氨苄西林(-)的 LB 培养板传代培养至第10代、20代、30代和40代时用灭菌的牙签分别挑取含有氨苄西林(+)和氨苄西林(-)LB 培养基上的单克隆菌落,质粒提取、PCR 扩增酶切鉴定；TPIN 转染 HepG2细胞后采用 RT-PCR 检测IL-2和 NK4基因表达,ELISA 法检测细胞培养上清 IL-2和 NK4蛋白。结果构建菌株经提取质粒、酶切、PCR 扩增获得目的基因 IL-2、NK4特异条带；在氨苄西林(+)和氨苄西林(-)LB 培养板传代培养40代的 TPIN 菌株均可扩增并双酶切出目的基因 IL-2及 NK4；TPIN 体外转染 HepG2细胞后,IL-2及 NK4表达水平均显著升高。结论重组携带IL-2/ NK4双基因的减毒沙门菌菌株 TPIN 可稳定遗传,不受无选择性压力的影响而丢失质粒,且在体外 IL-2、NK4基因及蛋白可以稳定高效表达。

Objective To prepare an attenuated Salmonella typhimurium DNA vaccine containing pCMV-IL-2-IRES-NK4 plasmid (TPIN) and to detect its stability. Methods The pCMV-IL-2-IRES-NK4 plasmid was transformed into an attenuated Salmonella typhimurium Ty21a competence, and then a recombinant attenuated Salmonella typhimuri-um strain TPIN was prepared; the recombinant strain was cultivated in 40 generations on LB nutritional medium plate with or without Ampicillin, and monoclonal bacterial colony was selected at subculture 10th , 20th , 30th and 40th generation from Ampicillin ( + ) and no Ampicillin ( - ) plates respectively, and then the plasmids were extracted and identified by PCR amplification and enzyme digestion; TPIN was transfected into HepG2 cells, then expressions of the IL-2 / NK4 genes were determined by reverse transcription polymerase chain reaction (RT-PCR), and expressions of IL-2 / NK4 albumen cells were detected by enzyme linked immunosorbent assay (ELISA). Results Goal genes IL

目的建立注射用哌拉西林钠舒巴坦钠有关物质测定方法。方法采用C18柱;流动相为:水、乙腈、0.2mol/L磷酸二氢钾溶液,线性梯度洗脱;检测波长为220nm。结果线性范围为舒巴坦1.5~36.3μg/mL,1-乙基哌嗪-2,3-二酮1.7~40.6μg/mL,氨苄西林1.4~34.5μg/mL,哌拉西林1.6~38.2μg/mL;重复性为1-乙基哌嗪-2,3-二酮RSD 4.8%,氨苄西林RSD 14.1%,杂质ⅡRSD 4.1%;1-乙基哌嗪-2,3-二酮的回收率均值为(98.9±7.8)%,氨苄西林回收率均值为(103.8±9.7)%;哌拉西林的定量限为4ng,检测限为1ng。样品溶液不稳定,需要配制后立即进样,耐用性试验显示需要选定专用色谱柱。结论本方法简便,专属性、重复性良好,可用于注射用哌拉西林钠舒巴坦钠有关物质的测定。

Objective To establish a method for the determination of related substances of piperacillin sodium and sulbactam sodium for injection.MethodsThe C18 column was used; The moving phase included water, acetonitrile and 0.2mol/L monopotassium solution, and the linear gradient elution was used; The detection wavelength was 220nm. ResultsThe linear range was 1.5-36.3μg/mL for sulbactam, 1.7-40.6μg/mL for 1-ethyl-piperazine-2, 3-dione, 1.4-34.5μg/mL for ampicillin, 1.6-38.2μg/mL for piperacillin respectively; The repeatability was RSD 4.8% for 1-ethyl-piperazine-2, 3-dione, RSD 14.1% for ampicillin and RSD 4.1% for impurity II respectively; The recovery rate was (98.9±7.8)% for 1-ethyl-piperazine-2, 3-dione and (103.8±9.7)% for ampicillin respectively; The quantitative limit of piperacillin was 4ng and the detection limit was 1ng. The sample solution was unstable and needed immediate sample introduction after preparation. Durability tests showed the need for dedicated chromato

目的探讨引起肺部感染的病原菌及其耐药状况。方法对187例肺部感染患者痰液标本进行病原菌鉴定及药敏试验检测,分析结果。结果 187份标本共检出病原菌164株,培养阳性率为87.70%,其中革兰氏阴性菌占71.95%,革兰氏阳性菌占18.29%,真菌占9.76%,革兰阴性菌中以铜绿假单胞菌、肺炎克雷伯菌、大肠埃希氏菌、肠杆菌属、鲍曼不动杆菌为主,革兰氏阳性菌中以金黄色葡萄球菌、肺炎链球菌、溶血性葡萄球菌为主,真菌主要为白色假丝酵母菌,占7.93%;检出的革兰氏阴性菌对亚胺培南、美罗培南、阿米卡星耐药率低,铜绿假单胞菌对氨苄西林、氨苄西林/舒巴坦完全耐药,肺炎克雷伯菌对氨苄西林完全耐药,肠杆菌属对头孢呋辛酯完全耐药,鲍曼不动杆菌对氨曲南、头孢呋辛酯完全耐药;检出的革兰氏阳性菌对万古霉素、夫西地酸、莫西沙星耐药率低,金黄色葡萄球菌对红霉素、克林霉素完全耐药,肺炎链球菌对红霉素完全耐药,溶血性葡萄球菌对青霉素、氨苄西林、氨苄西林/舒巴坦、亚胺培南、红霉素、克林霉素、环丙沙星、庆大霉素、磷霉素均完全耐药。结论引起肺部感染患者的病原菌主要以铜绿假单胞菌、肺炎克雷伯菌、大肠埃希氏菌、肠杆菌属、鲍曼不动杆菌为主的革兰氏阴性菌为主,检出病原菌均对临床常

Objective To investigate the cause of lung infection pathogens and drug resistance. Methods The sputum specimens of 187 cases of lung infection were collected for pathogen identification and susceptibility test. Results 164 strains of pathogens were detected, and the culture positive rate was 87. 70%, which included Gram-negative bacteria (71. 95%), Gram-positive bacteria (18. 29%), fungi (9. 76%). Gram-negative bacteria mainly included pseudomonas aeruginosa aeromonas, klebsiella pneumoniae, escherichia coli, enterobacter and acinetobacter baumannii. Gram-positive bacteria mainly included staphylococcus aureus, streptococcus pneumoniae and hemolytic staphylococci. Fungi mainly included candida albicans. Gram-negative bacteria showed a low resistance to imipenem, meropenem and amikacin. Pseudomonas aeruginosa showed a complete resistance to ampicillin and ampicillin/sulbac-tam. Klebsiella pneumoniae was completely resistant to ampicillin, and enterobacter complete resistant to cefuroxi