目的研究麻仁润肠丸泻下作用的效果及其机制,并和大黄、大黄素做比较,为麻仁润肠丸的临床应用提供理论依据。方法 96只SD大鼠随机分为正常对照组、麻仁润肠丸组、大黄组和大黄素组,用于检测大鼠一般状况、肠道传输功能;另96只大鼠随机分为正常对照组、麻仁润肠丸组、大黄组和大黄素组,检测肠壁组织、大肠运动频率与幅度、肠壁肠肌丛内神经的变化情况。数据均以平均数±标准误表示,每个实验至少重复三次。组间单变量统计分析采用one-way ANOVA法;组间两个单独变量统计分析采用Tukey post hoc检测以及two-way ANOVA法,以P0.05为差异有统计学意义。结果麻仁润肠丸长期给药对大鼠的泻下效果可稳定保持、对肠道运动功能的影响较稳定、对肠壁损伤较小、对神经系统的影响亦较小。大黄和大黄素对大鼠的一般状态影响较大,对大肠黏膜、肠道运动功能和神经系统的影响亦较大。主要表现为皮毛的清洁度较差,活动量减少,虚弱无力,精神不佳;泻下效果和对肠道运动功能影响的变化较大,晚期效果弱于麻仁润肠丸;晚期肠壁组织的损伤较为严重;早期胆碱能神经的数目增多,晚期减少;氮能神经的数目早期减少,晚期增多。结论麻仁润肠丸泻下作用温和、长期应用治疗效果优于大黄、大黄素,而其副作用却低于大黄、大黄素,长期应用不会出现严重的毒副反应,适合便秘的老年患者长期应用。
Objective The aim of the present study was to investigate the purgative effect of Maren Runchang Wan and to identify the mechanism involved compared with rhubarb ,emodin.Providing theoretical basis for the clinical application of Maren Runchang Wan.Methods 96 SD rats were randomly divided into control group,Maren Runchang Wan group,rhubarb group and emodin group .Recorded the general condition , intestinal transmission function .Another 96 rats were also randomly divided into control group , Maren Runchang Wan group,rhubarb group and emodin group .Then detected the changes of intestinal wall tissues , frequency,amplitude and myenteric plexus nerve of intestines .Results In Maren Runchang Wan group,the changes of purgative effect , intestinal movement function , intestinal wall tissue and myenteric plexus nerve were slight after long-term drug.In rhubarb group and emodin group ,the changes of general condition were apparent .the main performances changes were that the purity of the fur