在同一生境下用种子苗、根茎苗繁殖光果甘草(Glycyrrhiza glabra L.)、胀果甘草(Glycyrrhiza inflata Bat.)、乌拉尔甘草(Glycyrrhiza uralensis Fisch.),并对其地上部分(株高)和地下部分(根数、根粗、根茎数和根茎粗)进行比较研究。结果表明:(1)3种甘草地上部分、地下部分生长特征大部分种子苗优于根茎苗。(2)3种甘草生长特性表现不一致。在株高方面,种子苗和根茎苗均为胀果甘草表现最优;在根数方面,种子苗和根茎苗均为乌拉尔甘草最优;在根茎数和根粗方面,种子苗为乌拉尔甘草最优,根茎苗为胀果甘草最优;在根茎粗方面,种子苗为乌拉尔甘草最优,根茎苗为胀果甘草最优。(3)3种甘草种子苗、根茎苗根粗和根茎粗均与株高呈相关关系。

Under the same habitat Glycyrrhiza glabra l., Glycyrrhiza inlfata Bat., and Glycyrrhiza uralensis Fisch. were propagated by seed seedlings and rootstock seedling. Their aerial part (plant height) and underground part (root number, root diameter, rootstock number and rootstock diameter) were compared. The results showed that:(1) the growth features of aerial part and underground part of most seed seedling of three kinds of licorice were better than that of root seedlings. (2) the growth characteristic of three kinds of licorice were different. In terms of plant height, seed seedling and rootstock seedlings of Glycyrrhiza inlfata were optimal;In terms of root number, seed seedling and rootstock seedlings of Glycyrrhiza uralensis were optimal;In terms of rootstock number and root diameter, seed seedling of Glycyrrhiza uralensis were optimal, rootstock seedlings of Glycyrrhiza inlfata were optimal;In terms of rootstock diameter, seed seedling of Glycyrrhiza uralensis were optimal, rootstoc

目的了解甘草种衣剂对一年生甘草中根长、根干重和甘草酸含量的影响,为甘草包衣技术的推广提供理论依据和技术支撑。方法采用刻度尺直接测量根长,烘干后测量根干重的方法,甘草酸含量测定采用高效液相色谱法,对甘草根部粉末的70%乙醇提取物进行分析。以十八烷基硅烷键合硅胶为填充剂,乙腈为流动相A,0.05%磷酸溶液为流动相B,进行梯度洗脱,检测波长为237nm。结果甘草种衣剂可以增加一年生甘草根长、根干重和甘草酸含量,包衣组甘草中的甘草酸含量比未包衣组的增加了0.01%。结论甘草种衣剂可以促进增加甘草根长和根干重,进而促进甘草根部的生长,HPLC法方法准确,灵敏度高,重复性好,成本低,可用于甘草中甘草酸含量测定,甘草种衣剂可以提高甘草中甘草酸含量,进而提高了甘草的品质。

Objective To understand Effects of seed coating agent on the growth and content of glycyrrhizic acid of Glycyrrhiza uralensis, providing theoretical basis and technical support for the promotion of seed coating technology. Method Analysis the 70% ethanol extract of flour of G. uralensis root by measuring the length of G. uralensis root with dividing ruler, measuring the weight of G. uralensis after drying, and measuring the content of glycyrrhizic acid using HPLC. Elution by using octadecyl bonded silica as filler, acetonitrile as mobile phase A, 0.05% phosphoric acid solution as mobile phase B, the detection wavelength was 237nm.Result Glycyrrhizae seed dressing can incresase the length and weight of Glycyrrhizae root and content of Glycyrrhizic acid, the coating group, the content of glycyrrhizic acid in the coated group increased by 0.01% than that of the uncoated group. Conclusion Seed coating agent can help increasing length and weight of root, and promoting the growth o

目的建立同时测定甘草中异甘草素和甘草素含量的方法。方法采用高效液相色谱(HPLC)等度洗脱和检测波长时间序列采样的方法,流动相为甲醇-水=(45∶55),检测波长0~25min时为276nm,25~70min时为360mn,流速为0.8mL/min,同时测定甘草中异甘草素和甘草素的含量。结果异甘草素和甘草素进样量线性范围分别为0.028~0.28μg和0.064~0.64μg,平均加样回收率分别为97.92%和98.27%,RSD分别为1.29%和1.67%。结论该方法准确、灵敏度高、重现性好、操作简单,可为甘草药材的质量评价提供参考。

Objective To establish the method for simultaneously determining the isoliquiritigenin and liquiritigenin contents in gly-cyrrhiza uralensis Fisch. Methods The isocratic elution and the detection wavelength time sequence sampling by HPLC were adopt-ed. The mobile phase was methanol - water(45 : 55),the detection wavelength was 276 nm for 0 - 25 min and 360 mn for 25 - 70 min and the flow rate was 0. 8 mL. min - 1. Under these conditions,the isoliquiritigenin and liquiritigenin contents in glycyrrhiza uralensis Fisch were simultaneously determined. Results Isoliquiritigenin(0. 028 - 0. 28 μg)and liquiritigenin(0. 064 - 0. 64 μg)had good lin-ear relation. The average recovery rates of the two components were 97. 92% - 98. 27% with RSD of 1. 29% - 1. 67% . Conclusion The method is accurate and highly sensitive with good reproducibility and easy operation,and can provide the reference data for the e-valuation of glycyrrhiza uralensis Fisch medicinal material.

研究甘草在食品中应用，能够提高甘草的综合利用价值，制造出更多更好具有甘草特色的食品。甘草是药食两用资源，生理活性成分主要有三萜类化合物、黄酮类化合物及甘草多糖类化合物三大类成分。甘草提取物味甜，具有抑菌、抗病毒、抗氧化等诸多功能，在食品添加剂中作为甜味剂和抗氧化剂在饼干、肉制品等食品中使用，因具有生理活性功能在保健食品中应用广泛。目前甘草及提取物在粮油制品、肉制品、饮料、糖果、果冻、果脯、瓜子、酱油等食品中都有应用和研究。

Study on application of the glycyrrhiza and its extractive in the food industry can improve the comprehensive utilization of glycyrrhiza, which can promote to create more and better food with glycyrrhiza characteristics. Glycyrrhiza is medicine and food resources, physiological active ingredients are triterpenoids, flavonoids and polysaccharide. The glycyrrhiza and its extractive taste sweetly, that have antibacterial, antiviral, antioxidant and many other functions, the glycyrrhiza and its extractive are used in biscuit, meat and other foods as a sweetener and antioxidant, which also has been widely applied in health food because of physiological active functions. Now the application of glycyrrhiza and its extractive in grain and oil products, meat products, beverage, candy, jelly, dried fruit, seeds, soy sauce and so on are researched widely.

目的：探讨甘草渣总黄酮体外对羟自由基(·OH)的清除作用。方法：利用电子顺磁共振(EPR)技术检测不同浓度甘草渣总黄酮和甘草查耳酮甲对体外·OH的清除能力。结果：甘草渣总黄酮在0.25～25 mg/mL范围内清除·OH能力随浓度增加呈上升趋势，且相同浓度时其清除·OH能力高于甘草查耳酮甲。结论：甘草渣总黄酮有明显清除自由基作用，该方法、结果可作为有效活性成分的初步筛选。

Objective: To study the functions of total flavonoids in glycyrrhiza residues to scavenge hydroxyl radical(·OH). Methods: Scavenging effects of total flavonoids and licochalcone from glycyrrhiza residues in different concentrations on hydroxyl radical in vitro were detected by electron paramagnetic resonance (EPR). Results: As the concentrations of total flavonoids from glycyrrhiza residues increased, scavenging effects were raised when total flavonoids from glycyrrhiza residues were between 0.25 and 25mg/mL, and the capacity of eliminating hydroxyl radical was higher than licochalcone when they were at the same concentration. Conclusion: Total flavonoids in glycyrrhiza residues could scavenge hydroxyl radical obviously, and the method could be taken as the first step to screen active ingredients.