登录

双语推荐:成员1

基于水稻增产基因OsTAW1,从大豆全基因组中鉴定出23个GmTAW1同源基因家族成员,分析了大豆GmTAW1基因家族成员的保守结构域,并利用MEGA 4.1软件构建了系统发生树。研究了大豆GmTAW1基因成员的表达特征,为大豆产量性状的分子技术改良提供了候选基因资源。
Twenty three GmTAW1 genes were identified through whole soybean genome using an yield gene Os TAW1 as a query sequence. Conserved domains were analyzed by sequence alignment,and phylogenetic tree was con-structed by MEGA 4. 1 software. Expression patterns of GmTAW1 family genes were investigated in different organs in soybean. The results could supply the gene resources to improve soybean yield trait by molecular technology.

[ 可能符合您检索需要的词汇 ]

目的:研究破坏性膜拜团体成员焦虑类型、健康水平之间的关系,为膜拜成员心理转归提供理论依据以及干预应用指导方法。方法:采用状态-特质焦虑量表和自评健康评定量表,对62名膜拜成员和69名正常人员进行比较分析;立足精神动力学对结果进行佐证、理论分析。结果:1膜拜成员特质焦虑得分(48.77±7.33)分,对照组人员得分(40.87±8.98)分,二者差异显著(t=5.54,P0.001);2膜拜成员的生理、心理、社会健康水平及健康总分与非膜拜人员呈显著性差异(t=-3.38,P0.01;t=-4.24,P0.001;t=-3.36,P0.01;t=-4.68,P0.001);3膜拜成员特质焦虑与心理健康维度、健康总分均呈显著负相关(r=-0.44,-0.38;P0.01)。结论:膜拜成员的焦虑水平高于正常人员,健康水平低于正常人员,膜拜成员的转归需要心理援助对策。
Objective:To explore the relationship between trait anxiety and health levels among cult members group,and provide theoretical basis for the psychological outcome and then explore intervention methods.Methods:62 cult members were selected as testing objects,with 69 normal social members as control group.They were both tested by the state-trait anxiety scale( STAI) and self-reported health rat-ing scale( SRHMS) ,then carried on the comparative research.Based on the Angle of psychodynamics to analyze and give result proof.Results:①There was significant difference between experiment persons and normal persons in trait anxiety(t=5.54,P<0.001);② There were significant differences between ex-periment persons and normal persons in physical,mental,social health and self-test health score( t=-3.38,-4.24,-3.36,-4.68;P<0.001);③Trait anxiety of cult members was negatively correlated with mental health level and self-test health score(r=-0.44,-0.38;P<0.01).Conclusion:Cult members''anxi

[ 可能符合您检索需要的词汇 ]

目的探讨人类白细胞相关抗原复合体的DRB1*07/09/11等位基因对广西肝癌高发区肝癌家族聚集性的影响。方法以配对方法选取广西肝癌高发区的肝癌高发家族成员、肝癌单发家族成员及无癌家族成员153例作为研究对象,应用聚合酶链反应/序列特异性引物(PCR-SSP)方法检测HLA-DRB1*07/09/11等位基因,统计分析各等位基因与原发性肝癌家庭聚集性的相关性。结果(1) HLA-DRB1*07/09等位基因在肝癌高发家族成员组、肝癌单发家族成员组和无癌家族成员组的基因频率分别为0.70%和2.00%、2.60%和19.00%、23.50%和16.30%(P>0.05);HLA-DRB1*11为2.60%、13.70%和11.10%(P 0.05)。结论(1) HLA-DRB1*11等位基因可能是广西肝癌高发区原发性肝癌发生的拮抗基因,其缺失可能是导致广西肝癌高发区肝癌家族聚集性原因之一。(2)HLA-DRB1*07、09、11等位基因与广西肝癌高发区乙肝病毒感染的易感性似无明显关联。
Objective To study the effect of HLA-DRB1*07/09/11 alleles on familial aggregation of hepatocellular carcinoma(HCC) in high hepatocellular carcinoma incidence area of Guangxi. Methods 153 family members from those families which have had two or more than two patients with hepatocellular carcinoma(FHHC group), 153 from those families which have had only one patient with hepatocellular carcinoma(FOHC group) and 153 from those families which have had none carcinoma patients(FNC group) were selected and matched as study object in the high hepatocellular carcinoma incidence areas of Guangxi. Polymerase chain reaction using sequence specific primer (PCR-SSP) was employed to tested HLA-DRB1*07, HLA-DRB1*09, HLA-DRB1*11 alleles;the correlation bewteen HLA-DRB1*07/09/11 alleles and familial aggregation of HCC in the high hepatocellular carcinoma incidence areas of Guangxi was analysed by statistical methods. Results (1) The gene frequency of allele HLA-DRB1*07 in the FHHC group,FOHC group and F

[ 可能符合您检索需要的词汇 ]

本文以业务流程再造(BPR)为理论基础,对天津市 H 区辖属的1个社区卫生服务中心的家庭医生团队原分工协作管理流程进行分析发现,样本团队成员彼此责任不明确,专业化分工不够,同时团队成员间的协作性不强,服务效率不高。应积极转变思路,推行新的家庭医生团队流程化管理模式,同时调整团队内部分工,对需合作的服务项目进行细化分工,强化团队成员的协作网络管理,加强团队之间、团队成员之间的沟通协作。
Based on the theory of business process reengineering(BPR),the article analyzed the labor division and collaboration management process of family doctor team in one community health center of H community,Tianjin. The result showed that the each other responsibility of the sample team members was vague,the specialization division of labor was low, collaboration between team members was loose and the service was inefficient. Therefore,we should actively change our thinking and look for new processed management model of family doctor team. At the same time we should adjust the team internal division of labor,make a detailed division of labor in the service items in need of cooperation,strengthen the team membersˊ cooperation network and the communication and cooperation between the teams and between team members.

[ 可能符合您检索需要的词汇 ]

为了进一步研究GH/PRL家族信号通路在鱼类早期胚胎发育中的作用,研究以斑马鱼为模型,通过Real-time PCR技术和原位杂交技术刻画并比较了GH/PRL家族成员及其受体家族成员在胚胎发育早期的表达模式。结果发现,在配体家族成员中,生长激素(Growth hormone,GH)和生长催乳素(Somatolactin,smtl)存在母源表达,在受体家族成员中,ghra、ghrb存在母源表达。利用荧光素酶分析spi2.1启动子活性的结果初步证明,在斑马鱼早期胚胎发育中,各配体家族成员与GHRa之间可以发生广泛的互作。这一系列结果对于我们认识GH/PRL家族信号通路在斑马鱼早期发育中的作用具有重要的指导意义。
Growth hormone (GH) is a member of the GH/Prolactin (PRL) protein super family. It is secreted by anterior pituitary and critically involved in the regulation of cell proliferation, differentiation and migration. Using RT-PCR (re-verse-transcription PCR), our previous study revealed that both gh and its receptor gene ghra (growth hormone receptor a) were maternally expressed in zebrafish. This suggested that the GH/GHR signaling pathway might play an important role at the early stage of zebrafish development. To further study the function of GH/PRL signaling pathway in zebrafish embryogenesis, here we characterized and compared the expression patterns of gh/prl and their receptor family members in early embryonic development using real-time PCR and in situ hybridization. We found that the ligand family mem-bers, gh and somatolactin (smtl), and the receptor gene family members, ghra and ghrb, were maternally expressed in zebrafish. The analysis of spi2.1 promoter activity indicated the
目的 通过对1个Carney综合征家系进行临床调查和分子生物学研究,根据此家系发病特点总结其遗传规律及临床特征,明确该家系PRKAR1A基因突变位点.方法 对该家系进行详细病史的问诊及体格检查,并进行相关生化检查.采集患者及其家系成员12例DNA样本,对PRKAR1A基因9个外显子及邻近的内含子进行测序.结果 包括先证者在内,该家系中共7名成员存在PRKAR1A基因内含子c.440+4delG突变.这7名成员均有口唇、颊黏膜、指端皮肤色素沉着这一特殊体征.结论 该家系中存在PRKAR1A基因内含子c.440+4delG杂合突变,该位点突变可能与该家系成员皮肤色素沉着这一表型相关.
Objective To identify PRKAR1A mutations in a pedigree with Carney''s complex through clinical investigation and molecular biology research,and to summarize the genetic law,characteristics,and clinical features of this family disease.Methods The family members received a detailed medical examination and related biochemical tests.The hereditary history and clinical features were recorded.DNAs of 12 family members were extracted from blood and 9 exons and adjacent introns of PRKAR1A were sequenced.Results PRKAR1A mutation intron4 c.440+4 delG was identified in 7 family members,including the proband''s patient,who presented special signs of pigmentation on the lips,buccal mucosa,and fingertips.Conclusions The deletion mutation (c.440+4del G) in intron 4 of the PRKAR1A gene was found in this family,which is possibly associated with the phenotype skin pigmentation.
[提要]对一个包括先证者在内共28位成员的甲状腺激素抵抗综合征家系进行临床调查,并提取先证者及其家系14位成员(其他13位成员拒绝抽血)外周血白细胞基因组DNA,对甲状腺激素受体(thyroid hormone receptor,TR)β基因的第110外显子进行PCR扩增,PCR产物进行DNA测序检测突变位点.测序结果显示,该家系中有3名成员TRβ第10外显子1 303位核苷酸发生C转换为T的错义突变,使该位点编码的氨基酸由组氨酸变为酪氨酸(H435Y),此突变为杂合子突变,可能导致了甲状腺激素抵抗综合征的发生.
[Summary] The genomic DNA was extracted from peripheral blood leukocyte of the patient with thyroid hormone resistance syndrome and 14 members of his family.The exons 1-10 of thyroid hormone receptor β (TRβ) gene were amplified by PCR.The products of PCR were sequenced directly to detect the gene mutation.The results showed that 3 members of this family were confirmed to have the C→T transition mutation at nucleotide 1 303 site within exon 10 of TRβ gene,and the missense mutation results in the substitution of histidine to tyrosine (H435Y).The heterozygous mutation may lead to the occurrence of thyroid hormone resistance syndrome.

[ 可能符合您检索需要的词汇 ]

回顾性分析1例经典型21-羟化酶缺乏症(21-OHD)患者的临床资料,采用PCR-DNA直接测序技术对患者及其家系成员与50例无血缘关系个体进行CYP21 A2基因遗传学分析.序列分析发现先证者存在复合杂合突变(剪切点突变IVS2-13A→G和错义突变Arg483Pro).家系成员中,3个父系成员携带剪切点突变IVS2-13A→G,3个母系成员携带错义突变位点Arg483Pro.提示在本家系中,复合杂和突变可能致病,单个杂合突变携带者无临床表型.患儿生长激素宜尽早应用.
Retrospective analysis of clinical data from a patient with classical 21-hydroxylase deficiency.The patient and her family members and 50 unrelated individuals were enrolled for genotyping in the CYP21A2 gene by PCR-DNA direct sequencing.A compound heterozygous mutation of IVS2-13 A→G and Arg483Pro was found in the proband.Among the family members,3 patrilineal family members were IVS2-13 A → G heterozygotes,the other 3 matrilineal family members were Arg483Pro heterozygotes.The result suggests that the carrier with compound heterozygotes mutation of CYP21A2l gene may lead to clinical symptom,while single heterozygotes mutation carriers showed no clinical phenotype.Children with 21-OHD should be treated with rh-GH as early as possible.

[ 可能符合您检索需要的词汇 ]

目的:探讨连续传代培养对人脐带间充质干细胞(hUC-MSCs)表达NOD样受体(NLR)家族所有23个成员mRNA的影响,为改进hUC-MSCs传代培养质量与增加hUC-MSCs在实验和临床应用中的数量及安全性寻找切入途径。方法:取剖宫产无菌新生儿脐带,经胶原酶II消化结合贴壁选择分离纯化hUC-MSCs,并连续传代培养;采用流式细胞术、诱导分化和RT-qPCR对连续培养传代前后的hUC-MSCs(第3和28代)进行鉴定并对23个NLR家族成员mRNA的表达进行定量分析。结果:连续传代培养前后hUC-MSCs的细胞表型均为CD29+/CD44+/CD105+/CD31-/CD34-/CD40-/CD45-/CD106-/HLA-DR-,能被诱导为成骨和脂肪细胞,符合国际细胞治疗协会建议的MSCs基本特征。全部NLR家族成员的mRNA在培养第3代hUC-MSCs上均有表达,且NOD1、NLRC4、NL-RC5、NLRP1、NLRP3、NLRP10、NAIP、NLRX1和APAF1高表达,其余成员低表达。培养传代至第28代除NLRP10的mRNA表达上升、NLRC5和NLRX1 mRNA表达基本不变外,其余成员的mRNA表达均有所下降,其中NLRP1 mR-NA表达差异有统计学意义(P<0.05)。结论:体外连续传代培养对hUC-MSCs表达NLR家族成员的影响是多向性的。这些影响与MSCs的增殖、分化及其免疫调节功能的联系有待进一步实验探讨。
AIM:To investigate the influence of continuous subculturing of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the mRNA expression of all 23 family members of NOD-like receptors (NLRs), and to search for the way of improving the subculture quality of hUC-MSCs and increasing the quantity and safety in the experimental and clinical application .METHODS:Neonatal umbilical cord was collected to isolate and purify the hUC-MSCs with the colla-genase II digestion and adherence screening methods .These cells were continuously subcultured .The hUC-MSCs at pas-sage 3 and passage 28 were identified by flow cytometry and induced differentiation .The mRNA expression of NLRs in the passage 3 and passage 28 hUC-MSCs was detected by RT-qPCR.RESULTS: The cell phenotypes of both passage 3 and passage 28 hUC-MSCs were CD29 +/CD44 +/CD105 +/CD31 -/CD34 -/CD40 -/CD45 -/CD106 -/HLA-DR-, and both of the cells were induced into osteoblasts and adipocytes , which were conformed to the criteria of Int

[ 可能符合您检索需要的词汇 ]

以新一代全球/区域多尺度通用同化与数值预报系统?热带气旋路径数值预报系统(global/regional assimilation and prediction system-tropical cyclone model, GRAPES-TCM)为试验模式,采用组合不同的物理参数化方案(MP)方法和随机全倾向扰动(STTP)方法,生成反映模式不确定性的集合成员,在此基础上设计包含6个成员的3种集合方案,方案1和方案3的成员分别用MP方法和STTP方法生成,方案2的成员同时采用MP和STTP方法生成,用3种集合方案对1109台风“梅花”进行了36次72h的集合预报试验。结果显示:对于路径预报,3种集合方案中预报效果最好的是方案3,其次为方案2,最差的是方案1;对于强度预报,方案1和方案2的预报效果差异不大,都远好于方案3。方案2和方案3的路径预报与强度预报都好于控制试验的预报,方案1的路径预报好于大部分成员的预报,强度预报好于所有成员的预报。3种方案的路径离散度都偏小,方案3偏小最多,其次为方案2;方案3的强度离散度也过于偏小,是3种方案中最小的,方案1和方案2的强度离散度在积分前期明显偏小,积分后期则有偏大的趋势,其中方案2的强度离散度大于方案1。与国内外8个业务数值模式的预报结果比较,对于路径预报,方案1优于5个业务模式的预报,方案2和方案3
The GRAPES-TCM (global/regional assimilation and prediction system-tropical cyclone model) is used to make ensemble prediction experiments for typhoon Muifa (1109) in 2011. Three kinds of ensemble schemes are designed for the experiments. Every scheme has six ensemble members, which reflect the uncertainty of the model. The method of multiple physics (MP) is used to form the members of scheme 1. The method of stochastic total tendency perturbation (STTP) is used to form the members of scheme 3. Both the MP method and the STTP method are used to form the members of scheme 2. Thirty-six experiments are made and the integration time is 72 h. The experiment results are as follows. In the three ensemble schemes, the track prediction of scheme 3 is the best, that of scheme 2 is the second, and that of scheme 1 is the worst. The intensity prediction of scheme 1 is close to that of scheme 2. They are both much better than that of scheme 3. The track and intensity predictions of schem

[ 可能符合您检索需要的词汇 ]